tecan ls series Operating Manual page 70

6. LSServer, Scanner Shell and Download
AGC
6-40 Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
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Pinhole combo box
Select the appropriate pinhole for measurement from the Pinhole combo box.
Tecan LS Series Scanners have three different pinholes (Small, Medium and
Large) that can be changed using the software. The size of the pinhole
determines the grade of confocality Select between confocal and non-
confocal measurements:
Small = confocal mode (focal depth: 70 µm)
Medium = less confocal (focal depth: 300 µm)
Large = non confocal (focal depth: 800 µm)
The small pinhole gives high background rejection and better signal/background
ratio. If samples are bulky or have a large volume, larger pinholes and less
confocality give better reproducibility and higher signals.
•
AGC Mode checkbox
Only available in:
Single Scan Mode
Dual Sequential Scan Mode
Not available in Dual Parallel Scan Mode due to Cross-Talk problems.
•
Noise Level % = amount of pixels in the percentage, which will be
discarded; Range: 0,01% - 1%
•
Signal Level % = Mean value of all pixels in the selected signal level range
will be set to 50.000
Range: 0,01% - 1%
See AGC Scan Area option button on page 6-16, for more information.
2004-11