tecan ls series Operating Manual page 109

2004-11 Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
All data needed for calculating crosstalk correction must be entered / selected in
the Crosstalk dialog box.
For information about how to get special data needed for crosstalk correction and
how to perform the correction, refer to the LSServer Beginners Guide manual .
In the Crosstalk dialog box, all data needed for crosstalk correction must be set.
The requested options must be entered in the corresponding edit boxes,
selections must be made in combo and list boxes.
The Crosstalk dialog box is divided into four areas:
Channel 1 / Channel 2 tab:
In the Channel 1 / Channel 2 tab, data must be entered, which has been
previously determined through special measurement (e.g. using Tecan LSA
Software for microarray analysis): Dye 1, Dye 2, K1, K2, Gain 1 and Gain 2.
Laser / Filter selection:
In the Laser / Filter combo box, laser and filter combinations for crosstalk must
be selected.
Current Date and Name edit box:
In the Date field the current date is displayed. In the Name edit box name or
initials must be entered
K1 / K2 data list box:
All data selected / entered in the Crosstalk dialog box is stored as a crosstalk
factor record (CrosstalkCor.dat). This can be added to K1 / K2 data list box.
Clicking on an item in the list box displays corresponding data. Crosstalk factor
records can be added, changed or deleted at any time.
Click OK to save all K1 / K2 list box data in a special file CrosstalkCor.dat and
close the dialog box.
The first part of Crosstalk dialog box is divided into individual tabs:
1. Channel 1 tab
Before calculating crosstalk correction a so-called K-factor must be
determined. This is done during a measurement without taking into account
crosstalk correction. Adjusted values in this measurement session must be
entered in the Channel 1 tab.
The Channel 1 tab contains the following elements:
Dye 1 In the Dye 1 edit box the description of used dye must be entered.
edit box
K1 In the K1 edit box the correction factor for contribution of dye with
edit box shorter emission wavelength into the detection channel of dye with
longer emission wavelength must be entered.
The K1 default value is set to 0. This means no crosstalk correction
will be done, no other parameters (Dye, gain) must be specified.
Gain 1 In the Gain 1 edit box PMT Gain channel 1 must be entered.
edit box
Gain 2 In the Gain 2 edit box PMT Gain channel 2 must be entered.
edit box
6. LSServer, Scanner Shell and Download
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