tecan ls series Operating Manual page 107

Gain Calibration...
2004-11 Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
To start the Gain Calibration wizard, select Gain Calibration... in the Service
menu. A wizard guides the user through the gain calibration procedure.
The Gain Calibration option is implemented in Scanner Shell as a wizard. It
represents workflow modules, which define a sequential procedure and guide the
user step-by-step to perform a specific complex activity.
The Gain Calibration is needed to take into account differences in sensitivity
between PMTs used in Tecan LS SERIES SCANNER instrument. It is NOT only
necessary for dual channel instruments. The deviation between PMTs can be up
to 500 %.
The LS SERIES SCANNER is provided with calibrated PMTs. Data from the
PMTs becomes less accurate over the course of its life-span, so gain calibration
should be repeated regularly: After 100 days the user will be requested to start
gain calibration again.
Gain calibration no longer valid after 100 days.
Tools needed for gain calibration are delivered with each instrument:
•
Scattered light glass slide
•
Calibration filter slide with different OD filters
For further information refer to the chapter The Gain Calibration Wizard on page
6-77 in this manual.
During the Gain Calibration procedure:
1. Door opens and filter slides are moved out.
2. The user is asked to prepare the instrument for Gain Calibration .
3. Instrument parameters are set for Gain Calibration .
4. Gain Calibration PMT1 is done.
5. Gain Calibration PMT2 is done.
6. Gain Calibration data PMT1 / PMT2 is saved
as GainCalibration.cvs in the CalData folder.
7. Door is closed and filter slides are moved out.
The gain calibration must be done for all PMTs found in instrument.
6. LSServer, Scanner Shell and Download
Note
Note
6-77