Subtract The Background - LI-COR C-DiGit User Manual

2. Click Auto Adjust and the shapes will move slightly to better enclose nearby
areas of fluorescence.

Subtract the Background

Subtract the background of the blot or gel from the shapes to obtain consistent
data. The software will not calculate Signal for the shapes if a background method
is not selected.
Click here to watch a video that demonstrates how to set the background
subtraction method.
1. Click the first button in the Background group and select Median from the drop-
down menu to subtract the median value of the pixels in the background
segment. This method is useful if the pixel intensities in the background
segment are not uniform.
Note: Select Average for the Background Method to subtract the average value
of the pixels in the background segment.
2. In the Background dialog set the Border Width to 3 and select Right/Left to
select the segments on the left and right sides of the shape.
3. Click Save.
Note: The black line under the curves in the Profiles window delineates the
background that will be subtracted.
Subtract a User-Defined Background
A region of the image can be chosen for the background.
Image Studio Software
C-DiGit User Manual
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