Low Signal; Troubleshooting - Image Studio Problems - LI-COR C-DiGit User Manual

Blot scanner
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6
Maintenance and Troubleshooting
Figure 6-2. Unwanted background caused by plastic wrap.

Low Signal

The C-DiGit Blot Scanner should be set to High Sensitivity to generate the highest
signal. Chemiluminescent substrates vary in quality and effectiveness, and using a
substrate from a different source may improve signal. Varying the primary and
secondary antibody dilutions may also affect the signal and background of a blot.
Troubleshooting – Image Studio Problems
Q: What image resolution can I export?
A: Image Studio can export images at resolutions up to 600 dpi in TIF, JPG, and
PNG format.
Q: Why doesn't my image import into Image Studio?
A: Image Studio supports TIFF, JPG and PNG files. These file formats come in
numerous forms. If your TIFF, JPG or PNG file does not import, please send the
image to LI-COR so that future updates will be compatible with your image file
format.
Q: Why is there a cyan color (grayscale image) or a bright white color (color
image) on my image in Image Studio?
A: The signal is saturated in these areas.
C-DiGit User Manual
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