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system notification that new hardware is found. Depending upon the system,
this notification may take place immediately when the cable is plugged in or it
may be a few minutes later.
During the Windows new hardware installation, the system may give the user a
choice between discovering drivers on the internet or from the local
installation. If the computer is connected to the internet, pick the selection
that looks for drivers on the internet. If the computer is not on the internet you
may need to specify the location. If the drivers are not found automatically,
choose 'Install from a list or specific location', and navigate to the <Program
Files\Licor\Image Studio Digits\driver> folder.
After installing the driver, start Image Studio and attempt to connect.
Front Panel Warning Light Indicates an Error
When the C-DiGit Blot Scanner is unable to complete a task requested by Image
Studio Application Software, or a hardware failure is encountered, the red error
indicator on the C-DiGit front panel (Figure 1-3) flashes and an audible alarm
sounds. This error condition is automatically cleared after a few seconds and a
message is displayed in the Image Studio Application Software status bar at the
bottom of the main window (below) to explain the error. Click on the
button to open the Status Message History dialog to view all diagnostic records.
The error message will instruct the user on any further required action.
Resetting the Instrument
In the event that the C-DiGit Blot Scanner becomes unresponsive, reset the
instrument by switching the rear power switch off for a few seconds, then back on.
This procedure cuts power to the instrument and should only be used when
communication cannot be re-established by any other means. The C-DiGit Blot
Scanner should resume normal operation after start up but may take several
minutes.
Troubleshooting – Chemistry Problems
High background can be caused by a variety of sources, including washing
methods, secondary antibody concentration, and substrate choice. Background
can be reduced by altering these sources.
High Background Due to Substrate
Pools of excess substrate on the membrane can lead to areas of high background,
as can adding more substrate to a membrane that has dried. Apply the substrate
Maintenance and Troubleshooting
C-DiGit User Manual
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Troubleshooting
