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LI-COR C-DiGit User Manual

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Summary of Contents for LI-COR C-DiGit

  • Page 1 ® Blot Scanner User Guide...
  • Page 2 CE Marking This product (model number 3600) is a CE-marked product. For conformity information, contact LI-COR Support at http://www.licor.com/bio/support. Outside of the U.S., contact your local sales office or distributor.
  • Page 3 The safety of this product can not be guaranteed if the product is used in any other way than is specified in this manual. The C-DiGit Blot Scanner is intended to be used by qualified personnel. Read this entire manual before using the C-DiGit Blot Scanner.
  • Page 4 This End- User License Agreement does not authorize you to use LI-COR’s or its licensors’ names or any of their respective trademarks.

  • Page 5: Table Of Contents

    Back Panel Scanning Surface Scanning Surface Safety Sample Placement Chapter 2: Quick Start Tutorial Chapter 3: Scanning Membranes Placing the Membrane on the C-DiGit Blot Scanner Scanning Surface Using Blocking Buffer Primary and Secondary Antibodies Washing Buffer Substrates Imaging Chapter 4: Computer Connections & Networking...
  • Page 6 Maintenance Troubleshooting –Hardware Problems System Requirements Cannot Connect to C-DiGit Blot Scanner USB Cabling Problems Front Panel Warning Light Indicates an Error Resetting the Instrument Troubleshooting –Chemistry Problems High Background Due to Substrate High Background from Wrapping the Blot in Plastic Wrap Low Signal Troubleshooting –Image Studio™...

  • Page 8: Chapter 1: Introduction

    The C-DiGit has one detection channel that detects visible wavelength emissions from chemiluminescent substrates. The C-DiGit Blot Scanner is a networkable device, and can be connected directly to a computer (Macintosh® or PC) via Ethernet or USB cables. Click here to watch an introductory video about the C-DiGit Blot Scanner.

  • Page 9: What's In The Box

    15-30 °C and a dew point no greater than 25 °C to prevent condensation on the instrument. Space Requirements The C-DiGit Blot Scanner requires an area 27.94 cm L (11.0") x 22.23 cm W (8.75") x 7.3 cm H (2.875", lid closed), or 31.12 cm, (12.25”, lid open).

  • Page 10: Safety Considerations

    WARNING: The C-DiGit Blot Scanner should not be used with any radioactive materials. Electrical Considerations The C-DiGit Blot Scanner includes a power supply that plugs into a wall outlet, with an output that plugs into the C-DiGit instrument. Do not locate the C-DiGit Blot Scanner where the power supply cord will be walked on or exposed to water or chemical spills.

  • Page 11: External Panels And Controls

    Figure 1-3. Front panel features and controls of the C-DiGit Blot Scanner. Power Indicator: While the C-DiGit Blot Scanner is performing its startup or shutdown procedure, the blue power indicator light on the instrument front panel blinks. When the blue indicator light is continuously on, the instrument is ready for operation.

  • Page 12: Back Panel

    Figure 1-4. The imaging surface is designed to scan a single membrane up to 10 cm x 8.5 cm. Scanning Surface Safety Be careful not to get your fingers caught between the C-DiGit instrument lid and the base of the scanning surface when the lid is closed. Do not put excessive pressure on the scanning surface.

  • Page 13: Sample Placement

    Sample Placement The C-DiGit Blot Scanner Imaging Surface has a clear window that delineates the edges of the C-DiGit Blot Scanner scanning surface. Membranes up to 10 cm x 8.5 cm placed within these markers will be fully imaged. Membranes can be placed anywhere within the imaging area to be scanned.

  • Page 14: Chapter 2: Quick Start Tutorial

    The Tutorial Guide is reproduced in this section, with some additional detail. 1) Unpack C-DiGit In addition to the C-DiGit Blot Scanner, you will find the items shown below (l-r): 1) Ethernet and 2) USB cables, 3) a power supply with interchangeable plugs, 4) a C-DiGit Target, and 5) Image Studio and Resource CDs.
  • Page 15 Chapter 2: Quick Start Tutorial Macintosh Users Note: Compatible with OS X 10.9 (Mavericks) or 10.10 (Yosemite). a. Place installation CD into drive. b. If installation does not start immediately, browse the Desktop and double- click on MacImageStudioDigits_5.x.x.dmg. c. Click Agree to accept the license agreement. d.
  • Page 16 12VDC 1A 5) Install the Power Supply Plug There are two power supplies available for use with the C-DiGit; you will have received either the Cincon (p/n 591-13058) or SL Power Electronics (p/n 591- 13032) power supply. Each of these power supplies is a universal 100-240VAC input, 12VDC power output supply with inter- changeable AC plugs for use in North America and Japan, Australia, Europe, and the United Kingdom.
  • Page 17 Chapter 2: Quick Start Tutorial Cincon Power Supply Align metal contacts on power supply and plug, and then slide down until plug locks into position N. America, Japan Australia Push button and United slide out to release Kingdom Europe SL Power Electronics Power Supply Turn clockwise to lock N.
  • Page 18 Plug the power supply into the wall outlet, and plug the power cord into the receptacle on the C-DiGit back panel (below). Connect the flat (Type A) connector on the USB cable to your com- puter, and the square (Type B) connector to the C-DiGit Blot Scanner back panel at the con- nector labeled ‘USB’.
  • Page 19 Chapter 2: Quick Start Tutorial 10) Create a New Work Area Folder The first time you start the software you will need to create the first Work Area. The Work Area is a folder on the hard drive or network where the images, analyzes and settings are stored. Click here to watch a video that demonstrates how to create a Work Area Folder.
  • Page 20 Work Area. 11) Connect to C-DiGit Blot Scanner If there is only one C-DiGit Blot Scanner detected by the computer, it will connect auto- matically; if there are multiple C-DiGit Blot Scanners detected, you can choose the desired C-DiGit Blot Scanner from a list of available instruments (below, listed by serial number).
  • Page 21 Chapter 2: Quick Start Tutorial Open the Target envelope and expose the side of the Target with the gray background to room light for 30 seconds. Place the Target face down (gray background) on the C-DiGit Blot Scanner scanning surface.
  • Page 22 Verify that your target image acquisition is similar to that shown below. If it matches, your C-DiGit is functioning correctly. NOTE: Store the Target in its envelope when not in use. You will generally not need to use the Target after this initial verification step; it may be used later, however, for diagnostic purposes if there is a problem with the instrument.
  • Page 23 Chapter 2: Quick Start Tutorial Click here to watch a video that demonstrates how to choose and adjust an acquired image. Click on the ribbon tabs (above) for additional features, including: Image - Adjust the image appearance, crop, rotate, view image array. Analysis - Draw shapes to quantify data.
  • Page 24 ® C-DiGit Blot Scanner Page 17...
  • Page 25 Page 18...

  • Page 26: Chapter 3: Scanning Membranes

    Generally speaking, chemiluminescent detection protocols for Western blotting do not need to be modified for use with the C-DiGit Blot Scanner. LI-COR does, however, have a variety of resources available for optimizing Western blots. Some of the most common questions regarding imaging with the C-DiGit Blot Scanner are listed below;...

  • Page 27: Using Blocking Buffer

    Chapter 3: Scanning Membranes Click here to watch a video that demonstrates how to place a membrane on the C-DiGit Blot Scanner. Using Blocking Buffer 1) Can I use Odyssey® blocking buffer to block my blot? Yes. Use only for the blocking step and be aware that the sodium azide from the Odyssey block- ing buffer may still be present on the membrane at the detection step and will bind to the HRP enzyme, resulting in reduced light production and less intense bands.

  • Page 28: Primary And Secondary Antibodies

    There are a wide variety of chemiluminescent substrates for HRP detection. In general, sub- strates with a faster reaction rate tend to be more sensitive. The C-DiGit Blot Scanner is designed to work well with most substrates. LI-COR carries a line of chemiluminescent sub- strates that have been optimized to work with the C-DiGit Blot Scanner;...

  • Page 29: Imaging

    Chapter 3: Scanning Membranes 2) How do I apply the substrate? Make sure the substrate is at room temperature before use. Substrate should be applied by fol- lowing the manufacturer’s suggested methods for the amount of substrate to use and the incub- ation time prior to imaging.
  • Page 30 ® C-DiGit Blot Scanner Overlaying the LUT histogram is a curve with three adjustable points (above right). Move the top Max Point to the left to map more of the higher intensity data to the brighter display pixels to make the bands appear brighter.
  • Page 31 Page 24...

  • Page 32: Chapter 4: Computer Connections & Networking

    Some networking configurations may require assistance from your IT professional. In general, there are two basic cabling configurations outlined below: 1) computer is connected to C-DiGit directly via the USB cable included, or; 2) computer is connected to C-DiGit directly via the Ethernet cable included. Page 25...

  • Page 33: Computer Connections And Networking

    Chapter 4: Computer Connections & Networking Computer Connections and Networking Direct Connection Via USB Cable Use the supplied USB cable (p/n 392-13201) to connect your computer and C-DiGit Blot Scanner, as shown below. Computer C-DiGit Blot Scanner USB Connection C-DiGit...

  • Page 34: Chapter 5: Maintenance & Troubleshooting

    Troubleshooting –Hardware Problems System Requirements The C-DiGit Blot Scanner and Image Studio will run on Windows 7, 8 and 8.1, or on Mac OS 10.9 or 10.10. Cannot Connect to C-DiGit Blot Scanner 1) Install the Image Studio Software before you try to connect the USB or Ethernet cable to the C-DiGit Blot Scanner.

  • Page 35: Usb Cabling Problems

    5) IPv6 must be enabled (Windows 7, 8, and 8.1). USB Cabling Problems If you are experiencing problems connecting to the C-DiGit Blot Scanner using the USB cable, note the following: The Image Studio installer for C-DiGit includes the required USB RNDIS driver.

  • Page 36: Resetting The Instrument

    High Background from Wrapping the Blot in Plastic Wrap Blots can be imaged directly on the C-DiGit Blot Scanner without wrapping in plastic wrap. In general, the membrane should not dry out in the amount of time it takes to complete a scan.

  • Page 37: Low Signal

    A: Image Studio supports TIFF, JPG and PNG files. These file formats come in numerous forms. If your TIFF, JPG or PNG file does not import, please send the image to LI-COR so that future updates will be compatible with your image file format.

  • Page 38: Obtaining Technical Support

    A: Yes. Image Studio 5.x for C-DiGit is currently supported on Windows 8.0, 8.1, and 8.1 update 2 (also known as 8.2). Q: When I try to connect to the C-DiGit Scanner, I see a list of multiple instruments. How do I know which one to choose? A: If there are multiple C-DiGit Blot Scanners connected, you will have to choose the desired C-DiGit Blot Scanner from a list of available instruments listed by serial number.
  • Page 39 Chapter 5: Maintenance & Troubleshooting 1) Click the Image Studio Application Button, point to Logs, and then click Export. The Zip Application Log Files dialog will open. 2) In the Zip Application Log Files dialog, keep All log files selected (unless you need to save a smaller file due to email restrictions), and choose where to save the file.

  • Page 40: Chapter 6: Specifications

    Product Dimensions: 27.94 cm L x 22.23 w x 7.3 H (11” L x 8.75” W x 2.875” H) Weight: 2.18 kg (4.8 lbs.) Product Includes: C-DiGit Blot Scanner, target image, external power supply, Ethernet cable, USB cable, and software and resource CDs.
  • Page 41 Page 34...

  • Page 42: Chapter 7: Training Video Links

    Blot Chapter 7: Scanner Training Video Links The following are links to training videos for the C-DiGit Blot Scanner; these links are also present in this guide at appropriate locations. Scene 1–Introduction Scene 2.1 –Requirements for Image Studio Scene 2.2 –How to Install Image Studio on Windows Scene 2.3 –How to Install Image Studio on Macintosh...
  • Page 43 Chapter 7: Training Video Links Scene 8–Adjusting the Image Appearance Scene 9–Annotating Images Scene 10–Exporting Data Scene 11–Printing Lab Reports Scene 12–Importing Images Scene 13–Placing a Blot on the C-DiGit Blot Scanner Page 36...

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