Section 3. Scanning Membranes; Placing The Membrane On The C-Digit Blot Scanner Scanning Surface - LI-COR C-DiGit User Manual

Scanning Membranes
3
For Western blotting methods, nitrocellulose or PVDF membranes may be used.
LI-COR offers Odyssey nitrocellulose membranes (10 membranes; 0.22 μm, 7 cm x
8.5 cm) under part number 926-31090. There are some general tips for using
membranes:
IMPORTANT: Do not touch the membrane – handle only with clean forceps.
Lift the membrane only by the corners.
Keep the membrane wet if it is to be stripped and re-used. This can easily be
accomplished by wrapping the membrane in plastic wrap.
Use clean containers to avoid cross-contamination and reduce background.
Multiple membranes can be washed together, provided there is ample volume
so each membrane moves freely.
Generally speaking, chemiluminescent detection protocols for Western blotting do
not need to be modified for use with the C-DiGit Blot Scanner. LI-COR does,
however, have a variety of resources available for optimizing Western blots. Some
of the most common questions regarding imaging with the C-DiGit Blot Scanner
are listed below; more detailed support materials can be found at:
http://www.licor.com/bio/applications/quantitativeWesternBlots/supportMaterials.jsp
Placing the Membrane on the C-DiGit Blot Scanner Scanning
Surface
The imaging surface is designed to scan a single membrane up to 10 x 8.5 cm.
Place the membrane face down, with the top of the membrane facing the back of
the instrument.
C-DiGit User Manual 3-1