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C-DiGit
Blot Scanner
bottom of the main window (below) to explain the error. Click on the button to open the Status
Message History dialog to view all diagnostic records.
The error message will instruct the user on any further required action.
Resetting the Instrument
In the event that the C-DiGit Blot Scanner becomes unresponsive, reset the instrument by switching
the rear power switch off for a few seconds, then back on. This procedure cuts power to the
instrument and should only be used when communication cannot be re-established by any other
means. The C-DiGit Blot Scanner should resume normal operation after start up but may take
several minutes.
Troubleshooting –Chemistry Problems
High background can be caused by a variety of sources, including washing methods, secondary
antibody concentration, and substrate choice. Background can be reduced by altering these sources.
High Background Due to Substrate
Pools of excess substrate on the membrane can lead to areas of high background, as can adding
more substrate to a membrane that has dried. Apply the substrate carefully and wick off any pools of
substrate before imaging. Do not allow the membrane to dry.
A.Pooled Substrate
B. Substrate Addition to Dried Blot
Figure 5-1. High background due to excess substrate (A), and addition of substrate to a dried blot (B).
High Background from Wrapping the Blot in Plastic Wrap
Blots can be imaged directly on the C-DiGit Blot Scanner without wrapping in plastic wrap. In
general, the membrane should not dry out in the amount of time it takes to complete a scan.
However, to maintain moisture on the membrane for an extended amount of time, you can place a
clear, flat plastic covering on top of the chemiluminescent Western blot to keep the substrate in
contact with the HRP enzyme and to prevent the blot from drying out. It will also minimize
pooling of the substrate.
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Table of Contents
Troubleshooting
