Table Of Contents - LI-COR C-DiGit User Manual

Table of Contents

Section 1. Introduction
About C-DiGit Blot Scanner .............................................................................. 1-1
What's in the Box ............................................................................................. 1-2
Placement in the Laboratory.............................................................................. 1-2
Ambient Laboratory Conditions ................................................................... 1-2
Space Requirements.................................................................................... 1-3
Safety Considerations........................................................................................ 1-3
Chemical Safety .......................................................................................... 1-3
Electrical considerations.............................................................................. 1-3
Scanning Surface and Rear Safety Labels ..................................................... 1-3
Fuse Replacement ....................................................................................... 1-4
External Panels and Controls ............................................................................. 1-4
Front Panel ................................................................................................. 1-4
Back Panel.................................................................................................. 1-5
Scanning Surface ........................................................................................ 1-5
Scanning Surface Safety .............................................................................. 1-6
Sample Placement ...................................................................................... 1-6
Section 2. Quick Start Tutorial
Section 3. Scanning Membranes
Placing the Membrane on the C-DiGit Blot Scanner Scanning Surface ............... 3-1
Using Blocking Buffer ....................................................................................... 3-2
Primary and Secondary Antibodies.................................................................... 3-3
Washing Buffer ................................................................................................. 3-4
Substrates ......................................................................................................... 3-4
Imaging ............................................................................................................ 3-5
Section 4. Image Studio for C-DiGit
Overview ......................................................................................................... 4-1
Learning the Software.................................................................................. 4-1
Optional Key Tips ....................................................................................... 4-1
The Image Studio Main Window ....................................................................... 4-2
Quick Launch Buttons ...................................................................................... 4-3
The Application Menu ...................................................................................... 4-3
Save ................................................................................................................. 4-4
Save as PDF...................................................................................................... 4-4
Print ................................................................................................................. 4-5
Export............................................................................................................... 4-6
Single Image View ...................................................................................... 4-6
iv