LI-COR C-DiGit User Manual page 62

Blot scanner
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The adjustable curve overlaying the histogram represents how the raw data maps to
the display data. Adjust any of the three dots on the line (Min, Max, or K Value) to
optimize the displayed image. To view the numbers, hover the mouse over one of
the three circles on the line. The Max and Min numbers are displayed. The middle
circle also shows the K value, which is used to smoothly change between linear
(K=0) and logarithmic (K=1) mapping of the data onto the color maps.
Moving the Max Point to the left causes more of the intensity values to be mapped
to the brightest display values, making the bands brighter.
Moving the Min Point to the right shades more of the lower intensity values to
lower display values, creating a visually cleaner background.
Increasing the K value reduces the contrast between the higher and lower intensity
values, so less intense bands appear brighter while very bright bands are avoided.
Adjusting the Parameters
You can adjust the display parameters as described below.
Use the color buttons to change the channel's display color.
Use the gray scale buttons to display the channel in gray scale. This is
useful for displaying the image for reports that will be published.
Use the zoom buttons to zoom in or out on the range of pixel values.
Use the expand button to show the full histogram in full zoomed out view.
Use the Choose Display button to select the best display from six
choices.
Use the Adjust Display button to open a dialog in which to set the min
and max points of the display mapping.
Note: The Choose Display and Adjust Display dialogs automatically open after
a scan. Click the arrow (>) in the Display group to open the Display Options
dialog and disable the checkboxes to disable this feature.
Image Studio Software
C-DiGit User Manual
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