Imaging - LI-COR C-DiGit User Manual

Imaging

1. Can I wrap the blot in plastic wrap before imaging?
As long as the plastic is clear, clean, and wrinkle free (the plastic wrap may
cause unwanted background, especially if it is folded or handled roughly), the
blot can be wrapped in plastic before imaging.
2. Why are the bands on my blot so light?
Use the Look Up Table (LUT) in Image Studio to adjust how data are mapped to
the display pixels of your computer screen.
You can view the Image LUTs as either slider bars (the default view, below left)
or as histograms overlaid with a curve (below right). To hide this view, click
the double arrows in the top right corner.
Overlaying the LUT histogram is a curve with three adjustable points (below
left). Move the top Max Point to the left to map more of the higher intensity
data to the brighter display pixels to make the bands appear brighter. Move the
lower Min Point to the right to map the lower intensity data to the background
color, creating a visually cleaner background. The middle point smoothly
adjusts the mapping from linear to logarithmic. Changing to a more logarithmic
mapping reduces the contrast between the lower and higher intensity data, so
the appearance of less intense bands is improved while avoiding overly dark
bands.
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