LI-COR D-DiGit User Manual

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Summary of Contents for LI-COR D-DiGit

  • Page 1 Gel Scanner User Guide...
  • Page 3 CE Marking: This product (model number 3500) is a CE-marked product. For conformity information, contact LI-COR Support at http://www.licor.com/biotechsupport. Outside of the U.S., contact your local sales office or distributor.
  • Page 4 Notes on Safety LI-COR products have been designed to be safe when operated in the manner described in this manual. The safety of this product cannot be guaranteed if the product is used in any other way than is specified in this manual. The D- ™...
  • Page 5 WARRANTY OF ANY KIND WITH REGARD TO THIS MATERIAL, INCLUDING, BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. LI-COR shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance, or use of this material.
  • Page 6: Table Of Contents

    About the D-DiGit Gel Scanner FCC Compliance Statement Safety Precautions Initial Setup Unpack the D-DiGit Gel Scanner Computer Requirements for D-DiGit Image Acquisition Software Install D-DiGit Image Acquisition Software Unlock Scanner Connect Power and USB Cables Turn on the D-DiGit Gel Scanner...
  • Page 7 Care and Cleaning 1. Turn off the scanner and unplug the power cable from the AC outlet. 2. Wipe the top and sides 3. Wipe around the scan surface and under the handle 4. Wipe the scan surface with deionized water Chapter 5: Appendix Troubleshooting Specifications...
  • Page 9: Chapter 1: Safety Information And Initial Setup

    Scanner Safety Information and Initial Setup About the D-DiGit Gel Scanner The D-DiGit Gel Scanner is designed to scan gels stained with safe alternatives to Ethidium Bromide. The D-DiGit Gel Scanner is intended for use with the following stains: ®...
  • Page 10: Safety Precautions

    Chapter 1: Safety Information and Initial Setup Caution: Changes or modifications not expressly approved by the manufacturer responsible for compliance could void the user's authority to operate the equipment. This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.
  • Page 11: Using The Scanner

    D-DiGit™ Gel Scanner Do not hit or drop the scanner. The scanner may be damaged if it receives a strong jolt, which may result in fire or electric shock if the scanner is used without being repaired. Do not place the scanner on any slippery, slanted, or unstable surface. The product may slide or fall, causing serious injury to people as well as serious damage to the product.
  • Page 12: Initial Setup

    5. USB Cable 6. Quick start guide Computer Requirements for D-DiGit Image Acquisition Software The following specifications are required to run D-DiGit Image Acquisition Software: Color display with 24-bit color output capability 512 MB RAM or above 300 MB HDD or above Pentium IV PC or higher with Hi-Speed USB (USB 2.0)
  • Page 13: Unlock Scanner

    Turn on the D-DiGit Gel Scanner Turn the Power switch on the back of the D-DiGit Gel Scanner to "On". Once powered on, the D-DiGit Gel Scanner will be ready to scan.
  • Page 14: D-Digit Image Acquisition Software Overview

    Chapter 1: Safety Information and Initial Setup Once the control panel is active, you can use the D-DiGit Gel Scanner to scan, excise, or observe gels. D-DiGit Image Acquisition Software Overview D-DiGit Image Acquisition Software allows you to capture images from the D-DiGit Gel Scanner.
  • Page 15: Chapter 2: System Overview

    D-DiGit™ Chapter 2: Scanner System Overview Scan Surface and Lid View with the lid raised and the scan surface exposed. Part Name Description 1 Holes (2) Used to attach the Viewing Shield when performing a gel observation or band excision...
  • Page 16: Rear Panel

    Chapter 2: System Overview Rear Panel Part Name Description 1 Hi-Speed USB Port Connects the Hi-Speed USB (USB 2.0) cable to the computer. 2 Power Connector Connects the scanner to the power adapter. 3 Power Switch Turns the scanner on or off. Page 8...
  • Page 17: Chapter 3: Operation

    Before scanning, ensure the scanner lid has been closed tightly to prevent light leakage and poor quality scans. Scan Gel The scanner must be unlocked before scanning. See Unlock Scanner on page 5 for instructions. 1. Start D-DiGit Image Acquisition Software. 2. Click Image Capture on the Main Control Panel to image the gel. Page 9...
  • Page 18: Excising Or Observing A Gel

    After a scan is completed, the scanned image will be saved to the folder defined under Settings For information about changing the folder location, see Modify D-DiGit Image Acquisition Software Settings on page 11. Excising or Observing a Gel Follow the steps below to observe a gel or excise a band from a gel.
  • Page 19: Modify D-Digit Image Acquisition Software Settings

    2 minutes before using the light again. 5. Once you are finished, click Light Off to turn off the light and exit the countdown timer. Modify D-DiGit Image Acquisition Software Settings Click Settings on the Main Control Panel to adjust the following parameters.
  • Page 20: Image Format

    Thumbnail View. Prepare Scanner for Shipment Lock the D-DiGit Gel Scanner before shipping it. 1. Ensure the scanner is powered off, and the power cord is unplugged. 2. With the rear panel of the scanner facing you, tilt the scanner and locate the locking switch underneath the scanner.
  • Page 21: Chapter 4: Maintenance

    Chapter 4: Scanner Maintenance The D-DiGit Gel Scanner is designed not to require routine maintenance or calibration. You should clean the scanner thoroughly after each use. If you experience a problem with your scanner, please contact LI-COR for assistance. Do not attempt to fix or perform maintenance on the D-DiGit Gel Scanner, or you may endanger yourself or cause damage to the scanner.
  • Page 22: Wipe Around The Scan Surface And Under The Handle

    Chapter 4: Maintenance 3. Wipe around the scan surface and under the handle a. Moisten another large Kimwipe with warm water. Handle b. Thoroughly wipe down the plastic around the scan surface and under the handle. Plastic around c. Wipe dry with a Kimwipe. scan area 4.
  • Page 23: Chapter 5: Appendix

    1. When trying to scan, an error message says that the scanner can't be found. Ensure the scanner is powered on. See Turn on the D-DiGit Gel Scanner on page 5. 2. When trying to scan, an error message says "Code: -3, Scanner Hardware Error."...
  • Page 24: Specifications

    Chapter 5: Appendix Specifications Type Desktop flatbed gel scanner Light Source Blue LED Scanning Modes 16-bit grayscale (Approx. 65,536 shades of gray) or 8-bit grayscale (Approx. 256 shades of gray) Resolution 150, 300, or 600 dpi Sensitivity 0.04 ng/band Scanning Area 7"...

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