Using Blocking Buffer - LI-COR C-DiGit User Manual

Using Blocking Buffer

1. Can I use Odyssey
Yes. Use only for the blocking step and be aware that the sodium azide from
the Odyssey blocking buffer may still be present on the membrane at the
detection step and will bind to the HRP enzyme, resulting in reduced light
production and less intense bands.
2. Can I use milk-based blockers?
Yes. Milk-based blockers can be used for chemiluminescent detection but
should be avoided when detecting phosphoproteins or glycoproteins. Milk-
based blockers may contain endogenous biotin and glycoproteins, resulting in
higher background on the membrane.
Click here to watch a video that demonstrates how to place a membrane
on the C-DiGit Blot Scanner.
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blocking buffer to block my blot?
Using Membranes
C-DiGit User Manual
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