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Profiles Tab
1. After creating at least one shape on an image, select a shape with the selection
arrow until it changes to a dashed line.
2. On the right side of the screen, click the Profiles tab to view two graphs of the
pixels within a selected shape. The top graph is a cross section of the pixel
intensities from top to bottom. The bottom graph is a cross section of the pixel
intensities from left to right.
3. Adjust the size of the selected shape by dragging the arrows on the corners of
the shape. Notice how the graph changes.
Note: The small black marks (circled in red above) indicate the boundaries of the
shape. Move the shape around the band so that the small black marks are close to
the edges of the peak to capture the entire signal for that band.
Auto Adjust
The software moves selected shapes to fully enclose areas of fluorescence near the
shapes.
1. Drag a rectangle around the shapes to select them. Dotted lines indicate the
shapes are selected.
Image Studio Software
C-DiGit User Manual
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Troubleshooting
