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LI-COR C-DiGit User Manual

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Summary of Contents for LI-COR C-DiGit

  • Page 1 ™ Blot Scanner User Guide ®...
  • Page 2 The safety of this product can not be guaranteed if the product is used in any other way than is specified in this manual. The C-DiGit Blot Scanner is intended to be used by qualified personnel. Read this entire manual before using the C-DiGit Blot Scanner.
  • Page 3 This End-User License Agreement does not authorize you to use LI-COR’s or its licensors’ names or any of their respective trademarks.

  • Page 4: Table Of Contents

    Scanning Surface Safety ................1-6 Sample Placement ..................1-6 Section 2. Quick Start Tutorial Section 3. Scanning Membranes Placing the Membrane on the C-DiGit Blot Scanner Scanning Surface ....3-1 Using Blocking Buffer ..................3-2 Primary and Secondary Antibodies..............3-3 Washing Buffer ....................3-4 Substrates ......................
  • Page 5 Export Current Image .................. 4-7 Multiple Image View................... 4-9 Edit File Name .................... 4-11 Copy Image ....................4-12 Move Image....................4-14 Import ......................4-16 Image Studio....................4-17 Odyssey...................... 4-18 Pearl......................4-19 Other Image ....................4-20 Delete Images....................4-20 Work Area......................4-21 Switch ......................
  • Page 6 Section 6. Maintenance and Troubleshooting Maintenance ....................6-1 Troubleshooting – Hardware Problems.............. 6-1 System Requirements .................. 6-1 Cannot Connect to C-DiGit Blot Scanner............. 6-1 USB Cabling Problems ................6-2 Front Panel Warning Light Indicates an Error ..........6-3 Resetting the Instrument ................6-3 Troubleshooting –...
  • Page 7 Appendix A. Specifications ® Appendix B. Windows XP Installation Instructions Appendix C. Training Video Links...

  • Page 8: Section 1. Introduction

    Software (Section 4). The C-DiGit has one detection channel that detects visible wavelength emissions from chemiluminescent substrates. The C-DiGit Blot Scanner is a networkable device, and can be connected directly ® to a computer (Macintosh or PC) via Ethernet or USB cables, or can be connected through wired networks.

  • Page 9: What's In The Box

    If the acquired pattern matches the target image, the C-DiGit Blot Scanner is functioning as it should, and is ready to image your sample. Instructions for using the Target are given below in Section 2, Quick Start Tutorial.

  • Page 10: Space Requirements

    Introduction Space Requirements The C-DiGit Blot Scanner requires an area 27.94 cm L (11.0") x 22.23 cm W (8.75") x 7.3 cm H (2.875", lid closed), or 31.12 cm, (12.25”, lid open). Safety Considerations Chemical Safety LI-COR Biosciences recommends that all chemicals be handled carefully, and that safe laboratory procedures be followed at all times.

  • Page 11: Fuse Replacement

    Power indicator Red error condition indicator Green image acquisition indicator Figure 1-3. Front panel features and controls of the C-DiGit Blot Scanner. Image Acquisition Indicator: This green indicator light blinks during image acquisition and is continuously on at other times, as long as communication with the Image Studio software is maintained.

  • Page 12: Back Panel

    The C-DiGit back panel has the instrument ON/OFF switch and connectors for the USB and Ethernet cables and the power supply. 12VDC 1A Scanning Surface The scanning surface is accessed by opening the lid on the C-DiGit Blot Scanner (Figure 1-4). C-DiGit User Manual...

  • Page 13: Scanning Surface Safety

    The C-DiGit Blot Scanner Imaging Surface has a clear window that delineates the edges of the C-DiGit Blot Scanner scanning surface. Membranes up to 10 cm x 8.5 cm placed within these markers will be fully imaged. Membranes can be placed anywhere within the imaging area to be scanned.

  • Page 14: Section 2. Quick Start Tutorial

    The Quick Start Tutorial Guide included with the C-DiGit Blot Scanner is intended as a quick verification tool to ensure that the C-DiGit is working properly, before you proceed to scan your own membranes. The Tutorial Guide is reproduced in this section, with some additional detail.
  • Page 15 Win Image Studio Digits Installer_3.1.x.exe. 3. Click Agree to accept the license agreement. 4. Follow the instructions to install the Image Studio software. ® * Windows XP may require a more extensive installation procedure, found in Appendix B. C-DiGit User Manual...
  • Page 16 12VDC 1A 5. Install the Power Supply Plug There are two power supplies available for use with the C-DiGit; you will have received either the Cincon (p/n 591-13058) or SL Power Electronics (p/n 591- 13032) power supply. Each of these power supplies is a universal 100-240VAC input, 12VDC power output supply with interchangeable AC plugs for use in North America and Japan, Australia, Europe, and the United Kingdom.
  • Page 17 N. America, Japan Australia Push button and United slide out to release Kingdom Europe SL Power Electronics Power Supply Turn clockwise to lock N. America, Japan Push button and turn counterclockwise to release Australia United Kingdom Europe C-DiGit User Manual...
  • Page 18 USB Connection 7. Turn on C-DiGit Blot Scanner Power C-DiGit Blot Scanner on with the power switch on the back panel. The blue power light on the instrument front panel (below) illuminates and blinks when the instrument is first powered on, and stops blinking when the instrument has fully started up and is ready to use.
  • Page 19 Click the New Folder icon to create a new folder for the Work Area. Name the folder; this folder will now appear in the Available Work Areas window each time the software is opened. Select the folder and click Save to set it as the Work Area. C-DiGit User Manual...
  • Page 20 Quick Start Tutorial Click here to create a new folder Mac: Click . Navigate to where the folder will reside, click the New Create New Folder button, and name the folder. Click Choose C-DiGit User Manual...
  • Page 21 Work Area. 11. Connect to C-DiGit Blot Scanner If there is only one C-DiGit Blot Scanner detected by the computer, it will connect automatically; if there are multiple C-DiGit Blot Scanners detected, you can choose the desired C-DiGit Blot Scanner from a list of available instruments (below, listed by serial number).
  • Page 22 Status panel in the Image Studio window will display ‘Ready’. NOTE: C-DiGit Blot Scanner uses IPv6 and IPv4 Link-Local Addressing to automatically create an IP network without any user configuration or additional servers.
  • Page 23 Standard, click on the Sensitivity button and choose Standard from the pull- down menu (below). The image acquisition using Standard sensitivity takes approximately 6 minutes to complete. Click the Start button on the Acquire tab ribbon. C-DiGit User Manual 2-10...
  • Page 24 Verify that your target image acquisition is similar to that shown below. If it matches, your C-DiGit is functioning correctly. NOTE: Store the Target in its envelope when not in use. You will generally not need to use the Target after this initial verification step;...
  • Page 25 Table - Filter table columns, add headers, export data, etc. Click on the Shapes and/or Images tabs at the bottom of the main window to view: Shapes - Table of quantification data for selected image. Images - Table of acquired images, settings, and comments. C-DiGit User Manual 2-12...
  • Page 26 Quick Start Tutorial C-DiGit User Manual 2-13...

  • Page 27: Section 3. Scanning Membranes

    Generally speaking, chemiluminescent detection protocols for Western blotting do not need to be modified for use with the C-DiGit Blot Scanner. LI-COR does, however, have a variety of resources available for optimizing Western blots. Some of the most common questions regarding imaging with the C-DiGit Blot Scanner are listed below;...

  • Page 28: Using Blocking Buffer

    Using Membranes Click here to watch a video that demonstrates how to place a membrane on the C-DiGit Blot Scanner. Using Blocking Buffer ® 1. Can I use Odyssey blocking buffer to block my blot? Yes. Use only for the blocking step and be aware that the sodium azide from...

  • Page 29: Primary And Secondary Antibodies

    HRP enzyme to antibody varies as well, and may affect the detection of the target. If the secondary antibodies from one vendor are not working, trying antibodies from LI-COR or other vendors may be helpful. LI-COR offers the WesternSure™ Goat anti-Mouse HRP secondary antibody under part number 926-80010, and the WesternSure™...

  • Page 30: Washing Buffer

    There are a wide variety of chemiluminescent substrates for HRP detection. In general, substrates with a faster reaction rate tend to be more sensitive. The C-DiGit Blot Scanner is designed to work well with most substrates. LI-COR carries a line of chemiluminescent substrates that have been validated to work with the C-DiGit Blot Scanner;...

  • Page 31: Imaging

    Changing to a more logarithmic mapping reduces the contrast between the lower and higher intensity data, so the appearance of less intense bands is improved while avoiding overly dark bands. C-DiGit User Manual...
  • Page 32 Using Membranes To change the Image LUTs from histograms (Curves) to Brightness/Contrast sliders, and vice versa, click the arrow (>) on the Display group on the Image ribbon to open the Display Options dialog. C-DiGit User Manual...

  • Page 33: Section 4. Image Studio For C-Digit

    Image Studio for C-DiGit Overview Image Studio DiGits is a simple, easy to use Western blot imaging and analysis tool that features an intuitive, application-driven ribbon interface. Image Studio DiGits is compatible with both PC and Macintosh computers. This section provides a reference for the software features available on each ribbon interface.

  • Page 34: The Image Studio Main Window

    Application menu, Images and Shapes tables, and a button to launch the Help application, as shown below. Quick Launch Buttons Ribbon Tabs Application Help Menu Image Area Image Data Images/Shapes Tables Status Bar C-DiGit User Manual...

  • Page 35: Quick Launch Buttons

    (>) click the item to display a secondary menu in the rightmost panel. The secondary menu displays additional choices and their descriptions. Note: To access these commands with the keyboard, press the ALT key to display the Key Tips. C-DiGit User Manual...

  • Page 36: Save

    Click Save as PDF to open the Save as PDF dialog (below), where you can select the Table, Lab Book, or Images to save in PDF format. See ‘Print Images and Create Reports’ later in this section for more information. C-DiGit User Manual...

  • Page 37: Print

    Image Studio Software Print The Print menu has choices for configuring the printed output for images, tables, and/or Shapes Lab Books. See ‘Print Images and Create Reports’ later in this section. C-DiGit User Manual...

  • Page 38: Export

    Work Area. Open the Application menu. Click here to watch a video that demonstrates how to export data. Single Image View Click the Export menu and expand the Single Image View submenu. C-DiGit User Manual...

  • Page 39: Export Current Image

    Browse to navigate to a different folder. 5. Click Save when finished. Export Current Image Open the Application menu. Click the Export menu and expand the Single Image View submenu. Check Current Image. The Export Current Image dialog is displayed. C-DiGit User Manual...
  • Page 40 Click Reset Pixels or Reset Inches to return the Output to the Original Size. 2. Select the Publication Resolution and the Image Format. If the image is for viewing on a website or for attaching to an e-mail, choose 72 dpi and High Quality JPEG file or PNG file. C-DiGit User Manual...

  • Page 41: Multiple Image View

    Browse to navigate to a different folder. 5. Click Save when finished. Multiple Image View Click the Multi-Image mode button, and then open the Application menu. Choose Multiple Image View from the Export menu. The Export Multi Image View dialog (below) is displayed. C-DiGit User Manual...
  • Page 42 2. Select the Publication Resolution and the Image Format. If the image is for viewing on a website or for attaching to an e-mail, choose 72 dpi and High Quality JPEG file or PNG file. C-DiGit User Manual 4-10...

  • Page 43: Edit File Name

    5. Click OK when finished. Note: If you are exporting multiple images, be sure to include a unique value (e.g. Image ID). If you simply specify a filename, such as "My_Image~Date", as C-DiGit User Manual 4-11...

  • Page 44: Copy Image

    1. Select the images to Copy. 2. Open the Application menu. Hover over Export. 3. Expand the Copy Image menu and choose Folder or Zip File. If you choose Zip File the Zip File dialog (below) is displayed. C-DiGit User Manual 4-12...
  • Page 45 4. Accept the default folder and filename, create a new folder, or navigate to a folder to save the copied acquisitions. 5. Click Save when finished. The images and acquisitions are copied to a zip file in the location specified. If you choose Folder the Folder dialog (below) is displayed. C-DiGit User Manual 4-13...

  • Page 46: Move Image

    (This folder can be a different Work Area.) When you move the images, they are permanently removed from the Images Table in this Work Area. 1. Select the images that you want to move. 2. Open the Application menu. Hover over Export. C-DiGit User Manual 4-14...
  • Page 47 3. Choose Move Image from the Export menu. The message below is displayed. 4. If you want the selected acquisitions to be deleted from the Images table after the move, click Yes to continue. The Move Image dialog is displayed. C-DiGit User Manual 4-15...

  • Page 48: Import

    Work Area. Import The Import menu has choices for importing images and acquisitions (images plus ® analysis data) from the C-DiGit Blot Scanner application as well as from Odyssey ® or Pearl images. You can import acquisitions (images plus analysis data) created by Image Studio, ®...

  • Page 49: Image Studio

    This is useful if you have previously transferred data to a different location for archiving or you are transferring data from another Work Area. 1. Choose Image Studio from the Import submenu. The Import Acquisitions dialog (below) is displayed. C-DiGit User Manual 4-17...

  • Page 50: Odyssey

    System Software into the current default directory. The import creates a new image folder with appropriate image files. This does not import analysis data. 1. Choose Odyssey from the Import submenu. The Import Odyssey Image(s) dialog is displayed. C-DiGit User Manual 4-18...

  • Page 51: Pearl

    The Image ID field for the new image is assigned a new unique Image ID. Pearl Pearl imports one or more TIF files created in Pearl Software. 1. Choose Pearl from the Import submenu. The Import Acquisition(s) dialog (below) is displayed. C-DiGit User Manual 4-19...

  • Page 52: Other Image

    The Delete Images command deletes selected images in the Images Table. Select the Images to delete, and click the Delete Images button. NOTE: You can also right-click on the image row in the table and choose Delete Images from the pop- up menu. C-DiGit User Manual 4-20...

  • Page 53: Work Area

    Work Area. Import Settings Imports all Work Area settings from a chosen Work Area. Opens the Import Work Area Settings dialog (below), where you can choose an available Work Area from which to import the settings. C-DiGit User Manual 4-21...

  • Page 54: Instrument

    Address, internal software version, etc. Connect If there is only one C-DiGit Blot Scanner detected by the computer, it will connect automatically; if there are multiple C-DiGit Blot Scanners detected, you will have to choose the desired C-DiGit Blot Scanner from a list of available instruments (below, listed by serial number).

  • Page 55: Disconnect

    Disconnects from the currently connected instrument. Update Software Click to open the LI-COR Biosciences Image Studio Software support page with links to the latest upgrades for the C-DiGit Blot Scanner instrument (embedded) software. Logs The Logs menu contains choices for printing and/or exporting the application log files.

  • Page 56: Export

    (zipped), and the location to which to save the zip file. The zip file is automatically named EventLogs.zip. Update Click to open the LI-COR Biosciences Image Studio Software support page with links to the latest updates for Image Studio software. Import Key Adding additional keys is not supported in Image Studio for C-DiGit.

  • Page 57: General

    The maximum size of the displayed image is 2" x 1". If the logo exceeds either of those dimensions, it is scaled maintaining the aspect ratio. If you do not want a logo displayed on reports, leave the Logo Path blank. C-DiGit User Manual 4-25...

  • Page 58: Image Luts

    When the cursor is placed over the image in the main Image Studio window, enabling the check box allows you to enlarge or reduce the image using the mouse scroll wheel. C-DiGit User Manual 4-26...

  • Page 59: Xls Export

    Image Studio Software XLS Export Specifies how saturation (infinity) and non-numeric values (NaN) are expressed when exporting into an Excel (.xls) spreadsheet file. C-DiGit User Manual 4-27...

  • Page 60: Viewing And Manipulating Image Data

    You can view the Image Look Up Tables as either slider bars (the default view, below left) or as histograms overlaid with a curve (below right). To hide this view, click the double arrows in the top right corner. C-DiGit User Manual 4-28...
  • Page 61 This histogram is shaded with the color map in use for that channel. Also shown in the panel is an adjustable curve that maps raw data to display data. C-DiGit User Manual 4-29...
  • Page 62 Note: The Choose Display and Adjust Display dialogs automatically open after a scan. Click the arrow (>) in the Display group to open the Display Options dialog and disable the checkboxes to disable this feature. C-DiGit User Manual 4-30...

  • Page 63: Link Luts Across Filtered Images

    Profiles The Profiles tab displays a lane, band, or shape profile. When a band or shape is selected, the profile is displayed corresponding to the band or shape. C-DiGit User Manual 4-31...

  • Page 64: Concentration

    You can add concentration standards to any analysis. Click here to watch a video that demonstrates how to quantify data. C-DiGit User Manual 4-32...

  • Page 65: Acquire Ribbon

    Concentration column. Acquire Ribbon Click the Acquire tab to display the Acquire ribbon options. The Acquire ribbon is used to set the parameters for capturing the image from the membrane on the C-DiGit scanning surface. C-DiGit User Manual 4-33...

  • Page 66: Status

    Image Studio Software Status Mouse over the C-DiGit logo to display the C-DiGit Instrument Status bar, which shows the serial number and instrument software version of the instrument that is currently connected (below). If an instrument is not connected, the Status display will show ‘Not Available’, as shown above.

  • Page 67: Sensitivity

    You can also use the mouse wheel or use the scroll function of a keypad to zoom in or out. Click Restore to automatically fit the image to the window. C-DiGit User Manual 4-35...
  • Page 68 Channel to open the Choose Display window, where six versions of the acquired image are displayed, each using a different display algorithm. 1. Click the view with the best display. The Adjust Display dialog (below) will open for further adjustment. C-DiGit User Manual 4-36...
  • Page 69 Display Assistant (below). Select Signal at the bottom left to adjust the maximum point on the curve in the Look Up Tables (below), Background to adjust the minimum point, or Midtones to adjust the K value. C-DiGit User Manual 4-37...

  • Page 70: Adjust The Image Appearance

    1. Click the Image LUTs tab to view the Image Look Up Tables as either histograms overlaid with a curve (below left), or as slider bars (below right). To hide this view, click the double arrows in the top right corner. C-DiGit User Manual 4-38...
  • Page 71 Note: Further explanation of the K value can be found in the on-line Help system by clicking the question mark in the upper right-hand corner of the screen. To adjust the intensity of the image in the Brightness/Contrast view, click and drag each slider bar. C-DiGit User Manual 4-39...

  • Page 72: View Mode

    (Loop continuously). Move the slider to adjust the time each image is displayed. Create Click a button in the Create group to create a new image acquisition file with the designated action applied. The original image acquisition remains unchanged. C-DiGit User Manual 4-40...
  • Page 73 Click Free Rotate to open the Free Rotate Options dialog box and click the arrow buttons to rotate the image clockwise or counterclockwise. Warning: Free rotations change the image data for the new image and may affect quantification results. C-DiGit User Manual 4-41...
  • Page 74 Click Reduce Noise to open the Reduce Noise Options dialog box and select an option. Click OK. Warning: Reducing noise changes the image data for the new image and may affect quantification results. The Align Channels dialog box has no functionality with C-DiGit. C-DiGit User Manual 4-42...

  • Page 75: Crop The Image

    1. Click Apply to display the corners of the currently defined cropping area on the image. 2. Click Modify to open the Edit Image Crop Marks dialog (below). Two boxes appear as dotted lines on the image. C-DiGit User Manual 4-43...
  • Page 76 Note: The small crop marks appear on the image as long as Apply is selected. The cropping definition will apply to any print or export action. To remove the small crop marks and print or export the full image, click Apply again to toggle off. C-DiGit User Manual 4-44...

  • Page 77: Data Analysis (Shapes And Annotation Ribbons)

    To resize the shape, hover over any corner or side of the shape. Drag the double-sided arrow to resize. Note: To activate the double-sided arrow, the cursor must be on the selection tool (click Select in the Shapes group or press the Esc key on the keyboard). C-DiGit User Manual 4-45...
  • Page 78 The selected shapes move together. Note: You can also rotate shapes by selecting them, pressing the Ctrl key, and scrolling with the mouse, or by clicking the Rotate button in the Edit group of the Shapes tab. C-DiGit User Manual 4-46...

  • Page 79: Profiles Tab

    Auto Adjust The software moves selected shapes to fully enclose areas of fluorescence near the shapes. 1. Drag a rectangle around the shapes to select them. Dotted lines indicate the shapes are selected. C-DiGit User Manual 4-47...

  • Page 80: Subtract The Background

    3. Click Save. Note: The black line under the curves in the Profiles window delineates the background that will be subtracted. Subtract a User-Defined Background A region of the image can be chosen for the background. C-DiGit User Manual 4-48...

  • Page 81: Show

    In the Show group, enable all check boxes, indicating all items will appear on the image. To specify the location of the labels, click on the arrow at bottom right to open the Image View Labels menu. Annotate the Image Click the Annotation tab to open the Annotation ribbon. C-DiGit User Manual 4-49...
  • Page 82 Edit in the Text group to view the Text Properties menu for the selected item. Insert different text or add a border to the text with this menu. If the selected item is an arrow, click and drag the sides of the dashed box to change the size of the arrow. C-DiGit User Manual 4-50...

  • Page 83: Tables And Table Ribbon

    4. Click the corner button to select all of the images or press Ctrl+A on the keyboard. 5. Right-click any cell to view a menu with options that include copy an image, delete images, or view the properties of an image. C-DiGit User Manual 4-51...

  • Page 84: Add Or Delete A Column

    3. To add an Experiment column, for example, enable the check box, indicating that column will appear in the table. 4. To remove the Resolution column, for example, disable the check box and remove that column from the table. C-DiGit User Manual 4-52...

  • Page 85: Edit The Image Title

    Edit the Image Title 1. Click the Table tab to view the Table ribbon. 2. Click Add/Remove in the Columns group. 3. Click Use an additional image column with the Image ID to enable the check box. C-DiGit User Manual 4-53...

  • Page 86: Add Custom Columns

    1. Click the Table tab to view the Table ribbon. 2. Click the Customize icon in the Columns group to view the Custom Columns menu. 3. In the Custom Columns menu, replace Custom Field0 with Department. C-DiGit User Manual 4-54...

  • Page 87: Paste Data In A Column

    ‘Tutorial’. 6. Click Paste in the Data group, press Ctrl+V on the keyboard, or use the right- click menu to paste the data in the selected cells. C-DiGit User Manual 4-55...

  • Page 88: Paste Data From A Spreadsheet To A Column

    Note: No change will be made if the fields in a column are all the same. 2. To change the direction of sorting, click the small box in the header with the up or down arrow. Or, right-click on the header and select Sort Ascending or Sort Descending. C-DiGit User Manual 4-56...

  • Page 89: Filter The Table Manually

    One or more defined filters can be applied to the table to show only the images of interest. 1. Click the Table tab to choose the Table ribbon. 2. Click Define to view the drop-down menu of all of the column headings. Select Sensitivity to filter using the Sensitivity column. C-DiGit User Manual 4-57...
  • Page 90 2. Click one of the filtering phrases to show the Column Filter Analysis menu. 3. Select filtering phrases from the drop-down menu by clicking on the arrows next to each box, or type your own phrase. C-DiGit User Manual 4-58...

  • Page 91: View The Shapes Table

    Conc. Std. - Value of the concentration defined for this shape Concentration - Calculated value of the concentration based on the standards for the shape 3. Move, add, delete, sort, and filter the columns in the same way as in the Images Table. C-DiGit User Manual 4-59...

  • Page 92: Exporting Data

    1. Click the arrow (>) on the Data group to view the Data Options menu (see Step 1 above). Export all rows or selected rows. Click Save. If necessary, select the rows in the table to export. 2. In the Table ribbon, click Launch Spreadsheet to open the Launch Spreadsheet dialog. C-DiGit User Manual 4-60...
  • Page 93 Image Studio Software 3. Select the folder destination and enter a file name. Click Save. 4. Any application that reads these types of files will open them with the selected data and corresponding column headings. C-DiGit User Manual 4-61...

  • Page 94: Print Images And Create Reports

    1. To open the Print dialog, click the printer icon in the quick access tool bar, press Ctrl+P on the keyboard, or click the Image Studio Application Button, hover over Print, and click Print. 2. Choose your printer setup parameters and click Print to print the currently displayed image. C-DiGit User Manual 4-62...

  • Page 95: Save An Image As A Pdf File

    Image Studio Software Save an Image as a PDF File 1. Click the Image Studio Application Button and select Save As PDF. 2. Click Save to save the current image in .pdf format. C-DiGit User Manual 4-63...

  • Page 96: Print A Report

    Print a Report In the Print dialog, select Shapes Lab Book and click Print to print the default report for the current image. Click here to watch a video that demonstrates how to print lab reports. C-DiGit User Manual 4-64...
  • Page 97 Image Studio Software To create a custom report, click Add Lab Book… to open the Print Format builder dialog (below). C-DiGit User Manual 4-65...

  • Page 98: Save A Report As A Pdf File

    1. Click the Image Studio Application Button and select Save As PDF. 2. In the Save as PDF dialog box, select Shapes Lab Book and click Save to save the default report for the current image. C-DiGit User Manual 4-66...
  • Page 99 Image Studio Software To create a custom report, click Add Lab Book… to open the Print Format builder dialog. Follow the example above to create a custom report. Click Save. C-DiGit User Manual 4-67...
  • Page 100 Note: Use only the supplied USB 2.0 Type A to Type B cable or Cat. 5E RJ-45 Ethernet cable to connect the C-DiGit Blot Scanner and computer. The Ethernet port on the C-DiGit Blot Scanner is Auto-MDIX, so a crossover cable is not needed. When Image Studio Application Software starts, it searches for and discovers any C-DiGit Blot Scanners automatically.

  • Page 101: Section 5. Computer Connections And Networking

    Computer Connections and Networking Direct Connection Via USB Cable Use the supplied USB cable (p/n 392-13201) to connect your computer and C-DiGit Blot Scanner, as shown below. Computer C-DiGit Blot Scanner USB Connection C-DiGit USB Connection Direct Connection Via Ethernet Cable Use the supplied Ethernet cable (p/n 616-06116) to connect your computer and C-DiGit Blot Scanner, as shown below.

  • Page 102: Connection To Existing Lan

    Scanner to an Ethernet wall socket, and a second Ethernet cable (not included) to connect the computer to another Ethernet wall socket, as shown below. The C-DiGit Blot Scanner will be recognized in Image Studio, as long as the computer and C-DiGit Blot Scanner have been connected to the same existing network (same subnet).

  • Page 103: Maintenance

    Troubleshooting – Hardware Problems System Requirements The C-DiGit Blot Scanner and Image Studio will run on Windows XP, Vista, and 7, or on Mac OS 10.6, 10.7 and 10.8. Cannot Connect to C-DiGit Blot Scanner 1. Install the Image Studio software before you try to connect the USB or Ethernet cable to the C-DiGit Blot Scanner.

  • Page 104: Usb Cabling Problems

    Scanner, manually connect by clicking the Image Studio Application button, and choose Instrument->Connect. In the event that the C-DiGit Blot Scanner is unable to connect to a computer, your IT department may need to make specific configurations active on the computer for communication to occur.

  • Page 105: Front Panel Warning Light Indicates An Error

    After installing the driver, start Image Studio and attempt to connect. Front Panel Warning Light Indicates an Error When the C-DiGit Blot Scanner is unable to complete a task requested by Image Studio Application Software, or a hardware failure is encountered, the red error indicator on the C-DiGit front panel (Figure 1-3) flashes and an audible alarm sounds.

  • Page 106: High Background From Wrapping The Blot In Plastic Wrap

    (B). High Background from Wrapping the Blot in Plastic Wrap Blots can be imaged directly on the C-DiGit Blot Scanner without wrapping in plastic wrap. In general, the membrane should not dry out in the amount of time it takes to complete a scan.

  • Page 107: Low Signal

    Figure 6-2. Unwanted background caused by plastic wrap. Low Signal The C-DiGit Blot Scanner should be set to High Sensitivity to generate the highest signal. Chemiluminescent substrates vary in quality and effectiveness, and using a substrate from a different source may improve signal. Varying the primary and secondary antibody dilutions may also affect the signal and background of a blot.

  • Page 108: Obtaining Technical Support

    Q: Will Image Studio for C-DiGit work on Windows 8? A: No. Image Studio for C-DiGit is not currently supported on Windows 8. Q: When I try to connect to the C-DiGit Scanner, I see a list of multiple instruments. How do I know which one to choose?

  • Page 109: Specifications

    Product Dimensions: : 27.94 cm L x 22.23 w x 7.3 H (11” L x 8.75” W x 2.875” Weight: : 2.18 kg (4.8 lbs.) Product Includes: C-DiGit Blot Scanner, target image, external power supply, Ethernet cable, USB cable, and software and resource CDs.
  • Page 110 12VDC 1A 3. Install the Power Supply Plug There are two power supplies available for use with the C-DiGit; you will have received either the Cincon (p/n 591-13058) or SL Power Electronics (p/n 591- 13032) power supply. Each of these power supplies is a universal 100-240VAC input, 12VDC power output supply with interchangeable AC plugs for use in North America and Japan, Australia, Europe, and the United Kingdom.
  • Page 111 N. America, Japan Australia Push button and United slide out to release Kingdom Europe SL Power Electronics Power Supply Turn clockwise to lock N. America, Japan Push button and turn counterclockwise to release Australia United Kingdom Europe C-DiGit User Manual...
  • Page 112 USB Connection 5. Turn on C-DiGit Blot Scanner Power C-DiGit Blot Scanner on with the power switch on the back panel. The blue power light on the instrument front panel (below) illuminates and blinks when the instrument is first powered on, and stops blinking when the instrument has fully started up and is ready to use.
  • Page 113 Click ‘Yes, this time only’ to allow Windows to search for the drivers. Click Next. Click ‘Install the software automatically’. Click Next. If the drivers are not found automatically, choose ‘Install from a list or specific location’, and navigate to the <Program Files\Licor\Image Studio Digits\driver> folder. C-DiGit User Manual...
  • Page 114 Windows XP Installation Instructions If you see the following warning, click “Continue Anyway” to complete the installation. After the installation is complete, click Finish. C-DiGit User Manual...
  • Page 115 7. Reboot your computer Reboot your computer to activate the driver installation. NOTE: If you disconnect the C-DiGit Blot Scanner from one USB port and reconnect it at a different USB port, you must go through the driver installation process again and reboot your computer to activate the driver installation.
  • Page 116 Work Area. 11. Connect to C-DiGit Blot Scanner If there is only one C-DiGit detected by the computer, it will connect automatically; if there are multiple C-DiGits detected, you can choose the desired C-DiGit Blot Scanner from a list of available instruments (below, listed by serial number).
  • Page 117 If a connection is not automatically established, click on the Image Studio Icon in the upper lefthand corner of the main window (below), hover over “Instrument” and click “Connect”. When a connection is established, you will see the green indicator light illuminate on the instrument front panel (above). C-DiGit User Manual...
  • Page 118 Windows XP Installation Instructions 12. Proceed with Step 12 of Quick Start Tutorial (Section 2) If you have established a connection with the C-DiGit Blot Scanner, you can continue with the Quick Start Tutorial at Step 12, in Section 2. If you have been unable to establish a connection, further instructions are given below.
  • Page 119 Connection Problems Using USB Cable 1. Use only the USB cable included with the C-DiGit Blot Scanner. 2. Image Studio for C-Digit should be installed on Windows before the USB cable is connected between the C-Digit instrument and the PC.
  • Page 120 Image Studio again. To remove a Route entry, at the command prompt type “route delete 169.254.0.0 A.B.C.D, where A.B.C.D is the IP address of the standard network card in the PC and is not 169.254.X.X (below). C-DiGit User Manual B-11...
  • Page 121 Training Video Links The following are links to training videos for the C-DiGit Blot Scanner; these links are also present in this guide at appropriate locations. Scene 1 – Introduction Scene 2.1 – Requirements for Image Studio Scene 2.2 – How to Install Image Studio on Windows Scene 2.3 –...

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