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Agilent Technologies 8453 Operator's Manual

Uv-visible spectrophotometer
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Summary of Contents for Agilent Technologies 8453

  • Page 1 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 2: Warning

    Software is subject to Agilent Tech- nologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will receive no greater than Restricted Rights as Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 3 Operating Your Control Unit This chapter describes how to prepare your control unit for operation. Display Summary This chapter contains a summary of the different control and read-out functions. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 4 Part 4 Servicing the Temperature Controller This part is intended only for use by Agilent Technologies service personnel or other qualified engineers. It contains a description of the electronics, and information about the service, cables and connectors, and replacement parts of your temperature controller.
  • Page 5 Troubleshooting This chapter contains troubleshooting instructions for trained personnel. Identifying and Replacing Parts This chapter describes how to find parts information. Safety Information This chapter contains relevant safety information. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 6 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 7: Table Of Contents

    Removing other Cell Holders Viewing the Cell Holder Installing the Temperature-Controlled Cell Holder Connecting Cables Connecting Flow Cell and Heat Exchanger Setting Up Your External Sensor Viewing the External Sensor Connecting Cables Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 8 Turning off Line Power Using Functions Selecting Functions Setting Functions Resetting Functions Setting Temperature Example for Setting Temperature Example for Correcting a Wrong Set Temperature Changing Brightness Changing Units Turning off Peltier Element Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer...
  • Page 9 Messages Responding to Messages OVERLOAD! WARNING 1 WARNING 2 Display Messages OUT OF RANGE Status Messages Status Lamps Ready Error Stirrer Communicating GPIB Commands Communication Interface Remote Mode GPIB Control Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 10 SET – Set Cell Temperature SEU – Select Unit SPE – Speed STA – Status STR – Stirrer TEM – Cell Temperature TRA – Trigger Acknowledge TRG – Trigger Out TST – Test Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer...
  • Page 11 Keyboard Display Electronic Keyboard LEDs Control Logic 8-Character Display Power Amplifier Switch Controller, Current Sensor Switch H-Bridge, Peltier Element Sensor Electronic Current Source Multiplexer, Demultiplexer Measuring Principle +5 V Regulator, Voltage Divider Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 12 Changing Parts Changing Temperature Controller Board Changing Inner Cabinet Assembly Changing Cell Holder Cable Safety Information Safety Information General Operation Safety Symbols Radio Interference Manufacturer’s Declaration Herstellerbescheinigung Agilent Technologies on Internet Index Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer...

  • Page 13: Description

    GPIB Commands The temperature control accessory consists of the control unit and temperature-controlled cell holder for the Agilent 8453 UV-visible spectrophotometer for sample temperature control. The digitally controlled unit uses thermoelectric heating and cooling (Peltier principle) over a 60 °...

  • Page 14: Temperature Control Unit

    Temperature set resolution † Temperature Display Range -10 °C–120 °C Display resolution Electromagnetic stirrer speed range 40–1000 rpm * or equivalent in K or °F † or equivalent in K or °F Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 15: Temperature-Controlled Cell Holder

    20 °C–40 °C, ±0.2 K >40 °C–60 °C, ±0.3 K 0 °C–60 °C, ±0.1 K Temperature Reproducibility >60 °C, ±0.2 K Typical Step Response 5.5 °C/min heating 3.0 °C/min cooling Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 16: External Sensor

    GPIB Commands The GPIB commands are available to remotely control the temperature control unit via a GPIB connection by means of GPIB commands. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 17: Installing Your Controller

    Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual Installing Your Controller Choosing a Suitable Place Unpacking Your Controller Setting Up Your Control Unit Setting Up the Temperature-Controlled Cell Holder Connecting Flow Cell and Heat Exchanger Setting Up Your External Sensor...

  • Page 18: Choosing A Suitable Place

    220–240 VAC ±10% Line frequency 48–66 Hz Power consumption 90 VA maximum The Agilent 8453, the HP 8452A or HP 8451A spectrophotometers must be within 30 cm. N O T E Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 19: Unpacking Your Controller

    . . . T1.0 Amp fuse 2110-0007 . . . Secondary fuses: 3.0 Amp fuse 2110-0003 . . . 0.5 Amp fuse 2110-0012 . . . Tubing for heat exchanger 5041-2189 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 20: Unpacking The Controller Options

    If you have ordered the options for use with the temperature controller: Check the options against the list below. Table 5 Controller Options Description Option Check External Sensor #200 . . . Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 21: Setting Up Your Control Unit

    ATTENZIONE di controllo. Each control unit is identified by a 10-digit serial number on a label attached to the rear panel. The serial number of the control unit is: ....Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 22: Viewing The Control Unit

    Figure 1 Front View of the Control Unit safety symbol, “Selecting Voltage” on page 23 voltage selector cover line-power socket Figure 2 Rear View of the Control Unit Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 23: Selecting Voltage

    Assurez vous d’avoir déconnecté le câble secteur avant de changer le réglage du ATTENTION voltage. Assicurarsi che l’alimentazione elettrica sia scollegata prima di spostare il selettore ATTENZIONE di voltaggio. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 24: Selecting Fuse

    Assurez vous d’avoir déconnecté le câble secteur avant d’installer ou de remplacer ATTENTION un fusible. Assicurarsi che l’alimentazione elettrica sia scollegata prima di cambiare o inserire ATTENZIONE un fusibile. Table 4 for the part numbers. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 25 1 Insert the tip of a small screwdriver under the slot on selector cover and lift up the cover to access the fuse. fuse holder voltage setting voltage selector cover Figure 3 Details of Line-Power Socket Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 26: Connecting Cables

    12, “Troubleshooting” for making the correct type of cable. You must use a screened cable to prevent radio interference and to comply with the C A U T I O N Radio Interference Requirements. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 27: Setting Up The Temperature-Controlled Cell Holder

    To avoid spillage of liquids into the sample compartment: remove cells and disconnect or N O T E remove flow cells from your cell holder before you remove the cell holder. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 28: Removing The Multicell Transport Cell Holder

    2 Lift the cell holder out of the sample compartment. Viewing the Cell Holder heat sink rear mounting screw front mounting cable Figure 4 Top View of Temperature-Controlled Cell Holder Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 29: Installing The Temperature-Controlled Cell Holder

    1 Push the 25 pin connector of the temperature-controlled cell holder cable into the cell holder connector. 2 Tighten the screws on the connector. When removing cables hold the connector and do not pull on the cable. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 30: Connecting Flow Cell And Heat Exchanger

    2 Connect this tubing to the inlet (marked with ↓) of the flow cell. 3 Use the tubing supplied with your flow cell to connect to the inlet of the heat exchanger, and from the flow cell to your pump. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 31 ATTENTION Rincer ce support avec un solvant miscible avant utilisation. Può restare del solvente di lavaggio (propanolo) nel capillare della cella. Rimuoverlo PRECAUZIONE eventualmente con un solvente miscibile prima dell’uso. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 32: Setting Up Your External Sensor

    La sonda de temperatura del sensor externo está recubierta de vidrio, y es frágil. PRECAUCION La sonde de température du capteur externe est gainée de verre et est fragile. ATTENTION La sonda termica del sensore esterno è rivestita di vetro e fragile. PRECAUZIONE Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 33: Viewing The External Sensor

    You insert the temperature probe into your sample in the cell holder during operation. See Chapter 9, “GPIB Commands” for instructions about operation of the external sensor. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 34 Installing Your Controller Setting Up Your External Sensor Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 35: Simple Maintenance

    Cleaning Heat-Exchanger Tubing Do only the maintenance specified in this section. Other maintenance or repairs must be done by Agilent Technologies trained personnel. Maintenance can be dangerous. Damage caused by maintenance is not covered by warranty. Maintenance is divided into the following parts: •...
  • Page 36 Replacement parts you may need depend on the maintenance task. Table 9 Replacement Parts Description Agilent Technologies Part Number Cell holder 89090-62301 External sensor 89090-82100 Heat exchanger parts: Stainless steel cover 89090-0230 Adapter (KEL-F) 5021-1870 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 37: Changing Fuse

    Ensure that line-power cord is disconnected before removing the top cover of the WA R N I N G control unit. Stellen Sie sicher, daß der Netzstecker gezogen ist, bevor Sie die Abdeckung der WARNUNG Kontroll-Einheit entfernen. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 38 Assurez vous d’avoir déconnecté le câble secteur avant d’enlever le capot supérieur ATTENTION du boitier de contrôle. Assicurarsi che l’alimentazione elettrica sia scollegata prima di rimuovere il ATTENZIONE coperchio dell’unità di controllo. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 39 Simple Maintenance Changing Fuse 2 Remove control unit top cover by removing two screws at the rear, lifting up, and pulling backwards, see Figure Figure 7 Removing Control Unit Top Cover. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 40 4 Place the cover on the control unit locating the top onto the front panel, see Figure Figure 9 Replacing Control Unit Top Cover 5 Replace and tighten screws at the rear. 6 Connect line power cord and turn on line power. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 41 Follow the same procedure as above. If the control unit does not operate, and you hear the fan operating, you must call Agilent Technologies for service. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 42: Leaking Fittings On Heat Exchanger

    Si ce devait àtre le cas, laisser refroidir l’ensemble 5 à 10 minutes. Se l’unità di controllo ä stata usata, campione, cella e dissipatore possono essere ATTENZIONE molto caldi. In questo caso, aspettare 5 - 10 minuti affinché si raffreddino. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 43: Stage 1: Disassembling Heat Exchanger

    Achten Sie darauf, daß die Kapillare nicht beschädigt werden. ACHTUNG Tenga cuidado de no da§ar los tubos de acero inoxidable. PRECAUCION Prendre garde à ne pas endommager le tube d’acier inoxydable. ATTENTION Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 44 3 Remove the damaged fitting. 4 Replace a new fitting (see Table 9 for part number). 5 Replace new disks, gripper, and ferrule. 6 Reassemble heat exchanger, “Stage 4: Reassembling Heat Exchanger”. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 45: Stage 4: Reassembling Heat Exchanger

    5 Using your fingers tighten the fitting one half a turn more. Use pliers only if you have difficulty holding the fitting with your fingers. 6 Replace stainless-steel cover of the heat exchanger by replacing and tightening the screws. 7 Turn on control unit. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 46: Cleaning Cells

    Si ce devait àtre le cas, laisser refroidir l’ensemble 5 à 10 minutes. Se l’unità di controllo è stata usata, campione, cella e dissipatore possono essere ATTENZIONE molto caldi. In questo caso, aspettare 5 - 10 minuti affinché si raffreddino. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 47 Figure 11 Temperature-Controlled Cell Holder with Cell 1 Raise the clamp lever and lift out the cell. 2 Wash the cell. 3 Wipe the optical surfaces with lint-free cloth. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 48: Replacing Cell

    Avoid spillage of liquids in the sample compartment. N O T E After a spillage clean the surfaces of the cell holder to prevent liquid entering the base of the holder. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 49: Cleaning Heat-Exchanger Tubing

    Tubing is supplied with your temperature control accessory. See Table 4 on page 19 for the N O T E part number and “Connecting Flow Cell and Heat Exchanger” on page 30 for connecting the tubing. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 50 Simple Maintenance Cleaning Heat-Exchanger Tubing Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 51: Operating Your Control Unit

    Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual Operating Your Control Unit Before you Start Turning on Line Power Turning off Line Power Using Functions Setting Temperature Changing Brightness Changing Units Turning off Peltier Element Selecting Stirring Setting System Functions...

  • Page 52: Before You Start

    ATTENTION Rincer ce support avec un solvant miscible avant utilisation. Può restare del solvente di lavaggio (propanolo) nel capillare della cella. Rimuoverlo PRECAUZIONE eventualmente con un solvente miscibile prima dell’uso. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 53: Turning On Line Power

    If your control unit displays anything else or the Error lamp is on, refer to “Troubleshooting” on page 147 in your handbook. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 54: Checking Operation

    The Ready lamp turns off or remains off to indicate warming before the set temperature of 37°C is reached. When the cell temperature reaches 37°C the Ready lamp turns on. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 55: Turning Off Line Power

    Turning off Line Power Turning off Line Power To turn off line power, press line power switch on front panel. When you turn off line power all settings are stored. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 56: Using Functions

    Cell Temp xx.xC LEAR N O T E If the cell holder and external sensor are connected the displays may show: Cell Temp High! Cell Temp Low! Temp High! Temp Low! Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 57: Setting Functions

    Peltier element on and off. To clear the current value press C LEAR N O T E 4 Press E NTER Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 58: Resetting Functions

    Operating Your Control Unit Using Functions Resetting Functions To reset the function to the previous value: 1 Press C LEAR 2 Press E NTER Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 59: Setting Temperature

    If the Ready lamp was on it turns off. If the Ready lamp was off it remains off. To correct a wrong number press C to reset to the previous function value. LEAR N O T E Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 60: Example For Correcting A Wrong Set Temperature

    For temperatures less than 0°C use F or K units. Set temperature in °F or K and change N O T E units. keys changes the set temperature by ±0.1. Pressing U and D Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 61: Changing Brightness

    D b Press a number key, for example 4 . 3 Press E to set the value. NTER keys changes the brightness value by ±1. Pressing U and D Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 62: Changing Units

    Fahrenheit to Celcius after using the and D keys for setting the temperature. This is due to rounding up or down. Table 13 Fahrenheit Examples To Set: Press 14°F 32°F 98.6°F Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 63 Operating Your Control Unit Changing Units Table 14 Kelvin Examples To Set: Press 270 K 296 K Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 64: Turning Off Peltier Element

    5 Press E to set the value. NTER When the stirrer function is on the Stirrer lamp is on. keys changes the stirrer speed value by ±1. Pressing U and D Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 65: Setting System Functions

    You set the remote mode either through the GPIB with the command REM ON or through the remote keyboard function. “REM – Remote” on page 109 describes setting remote mode with the GPIB command. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 66: Changing Gpib Address

    GPIB address, for example 1 5 to change the GPIB address to 15. 4 Press E to set the value. NTER You may set the GPIB address in the range 1 through 31. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 67: Starting Self-Test Manually

    2 Use the U or D keys to display<newline> Self-test and press E NTER The self-test includes all diagnostic functions and you may receive all error messages, described in the service handbook. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 68: Hints For Operation

    Your temperature-controlled cell holder is designed to operate at 10°C below ambient temperature, however, it may operate at lower temperatures. For best performance, use small temperature changes, for example, 1° to 5°, when setting temperatures below ambient. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 69 Take care when heating a cuvette with its stopper in or with the temperature probe in. If the cuvette is tightly stoppered, an increase in pressure may damage the cuvette. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 70 Operating Your Control Unit Hints for Operation Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 71: Display Summary

    Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual Display Summary You display the different control and read-out functions using the U keys. Agilent Technologies...

  • Page 72: Normal Mode

    Displays original value of parameter or function, clears previous entry. LEAR 0 to 9 Displays number 0 to 9 in numeric entry field. Displays decimal point (.) in numeric entry field. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 73: Using External Sensor

    Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual Using External Sensor The external sensor gives more accurate temperature monitoring of your sample, when used with the cell holder and control unit. You use the glass-coated temperature probe to measure the actual temperature of your sample.
  • Page 74 2 Remove the cover from the temperature probe, by turning and pulling the cover and holding the top of the temperature probe. Do not pull on the cable to remove the cover. 3 Insert the temperature probe into the cell. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 75 You may want to increase or decrease the value for set temperature to give the exact N O T E temperature for your sample as measured by the Ext Temp display. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 76 Using External Sensor Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 77: Messages

    Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual Messages Responding to Messages Display Messages Status Messages Status Lamps The control unit may display messages for your attention. There are two types of messages: • Requiring some action, for example, pressing a key.

  • Page 78: Responding To Messages

    The control unit is in edit mode and you can change the temperature, see “Setting Temperature” on page 59. 2 Press E to set the value. NTER Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 79 Il n’y a pas de spécifications de précision à cette température. A 60°C, la précision de température est ±0.3°C. La précision de la sonde de température peut dériver lors de passages fréquents d’une haute (au dessus de 70°C) à une basse température. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 80: Warning 2

    You should remove your sample cell from the cell holder, because temperatures are not kept stable during self-test. 1 Press C to reset. LEAR 2 Press E to continue the self-test. NTER Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 81: Display Messages

    Temp 100.0C or Set Temp 0.0C depending on whether the entered value was too large or too small. The control unit is in edit mode and you can change the temperature, see “Setting Temperature” on page 59. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 82: Status Messages

    2 Start the self-test, see “Starting Self-Test Manually” on page 67. If error is displayed again call Agilent Technologies service and report the error number. Error message for temperature too high and too low: Cell Temp High! and Cell Temp Low!.

  • Page 83: Status Lamps

    Error lamp is on if there is an internal error. For operating errors there will also be an error display, for example OUT OF RANGE for non-settable temperature. Stirrer Stirrer lamp is only on when you select Stirrer ON. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 84 Messages Status Lamps Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 85: Communicating Gpib Commands

    Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual Communicating GPIB Commands Communication Interface Remote Mode GPIB Control Agilent Technologies...

  • Page 86: Communication Interface

    N O T E you read only <CR> <LF and EOI> in the computer. 2 Instructions to ask for a reply from the temperature control unit. Exceptions are TRA, EXT and TEM. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 87 When the computer receives a SRQ it uses the serial poll capability to read the status byte of the temperature control unit. The SQR line can be activated automatically by the temperature control unit, see “Service Request” page 90. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 88: Remote Mode

    You may set the remote mode through the keyboard or the GPIB command and reset using N O T E the GPIB command. However, if you set the remote mode through the GPIB and reset through the keyboard an error message is generated: ERR 147. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 89: Gpib Control

    ASCII the computer from the out-buffer, which is also 256 byte of reserved space in main , through the I/O buffer. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 90: Service Request

    “STA – Status” on page 113 and “GPIB Control” on page 89. This bit must always be set back by the computer using the TRA command. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 91: Device Clear

    "selected device clear" message (GPIB commands) it will clear the in-buffer and out-buffer. It also clears the status byte and the service request mask. All stored errors are lost by resetting the communication interface. N O T E Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 92 Communicating GPIB Commands GPIB Control Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 93: Gpib Commands

    Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual GPIB Commands Buffers Instruction Format CSM – Agilent ChemStation Mode ERR – Error EXT – External Temperature IDY – Identify MSK – Service Request Mask PEL – Peltier REM – Remote SET –...

  • Page 94: Buffers

    The status of the buffer is represented by the ready reply bit of status byte (only using the serial poll to the computer). The buffer must be empty before another instruction can be executed successfully. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 95: Instruction Format

    Multiple instructions may be sent in an instruction string. However, for instructions which generate a reply from the temperature control unit only 1 is allowed and it must be the last in the instruction string. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 96: Detailed Description

    All instruction parameters are digits (ddd), or characters (ccc), and ASCII for temperature parameters dash (–) for minus, plus sign (+) for plus and fullstop (.) for the decimal point. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 97 25.7 is read by the temperature control unit. However, in replies for temperature values from the temperature control unit, two number after the decimal point are sent, for example, ddd.dd. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 98: Csm - Agilent Chemstation Mode

    0 or 1 (in ASCII The meaning of this number for the different commands is as follows: PELTIER OFF REMOTE OFF C (Centigrade) STIRRER OFF OUTTRIG LOW PELTIER ON REMOTE ON Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 99 F (Fahrenheit) Reply Description The CSM instruction without any parameters is to read the current mode of the interface. The meaning of the replied number is: normal mode Agilent ChemStation mode Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 100: Err - Error

    After the temperature control unit detects a hardware or device error the number is written in the , the number code is displayed on the front EEPROM panel, and the Error LED turns on. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 101 117 AMP0_OR_H 118 AMP1_OR_H 119 H_BRI0_COL 120 H_BRI1_COL 121 H_BRI0_HEA 122 H_BRI1_HEA 123 DISPLAY 124 MEMORY 125 ROM 130 EXT_SENS 131 INT_SENS0 132 INT_SENS1 133 CELL_HOLH0 134 CELL_HOLH1 135 COMMUNICATION Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 102 The temperature control unit has detected an error in the value (syntax or size) of a parameter that accompanied the most recent instruction. If the value is out of range, the previous value is kept. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 103 156 L_TEMP_SENS1 These operation errors are similar to the messages given by the temperature control unit as display messages without the numbers. For more details see Chapter 8, “Communicating GPIB Commands”. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 104: Ext - External Temperature

    (–999.99 | 999.99) regardless of the selected units. The out of limits result could be caused by a defective sensor. See also “Messages” N O T E page 77. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 105: Idy - Identify

    None Reply AGILENT89090A,REV x <CR> <LF and EOI> Description This instruction is used to retrieve a string that identifies the temperature control unit and the current revision of firmware installed. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 106: Msk - Service Request Mask

    After power on or "device clear" command the mask is always zero. The following numbers are the decimal values corresponding to the bits in the mask. If combinations of bits are enabled intermediate numbers are obtained. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 107 The computer should always evaluate the status byte after receiving a service request from N O T E the temperature control unit to determine the reason for the interrupt before performing any action. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 108: Pel - Peltier

    Peltier element. Reply Description “CSM – Agilent ChemStation Mode” on page 98 for interpreting the reply when using the CSM command. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 109: Rem - Remote

    = remote mode off = normal mode “CSM – Agilent ChemStation Mode” on page 98 for interpreting the reply when using the CSM command. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 110: Set - Set Cell Temperature

    Without any unit parameter the returned value is in the current instrument unit (for instruction see “SEU – Select Unit” on page 111). The format of the reply is: tem = [-][dd]d.dd Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 111: Seu - Select Unit

    SEU without any parameters generates a response with the current unit. Reply Description “CSM – Agilent ChemStation Mode” on page 98 for interpreting the reply when using the CSM command. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 112: Spe - Speed

    (40 -1000 rpm) a 144 PARA_RANGE error is generated and the speed is kept at the previous value. Reply Description The SPE instruction without parameters returns the current speed setting in rpm. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 113: Sta - Status

    1 to 0 transition of the trigger input line, INTRIG, pin 4 on the remote control connector. A trigger acknowledge (TRA) instruction clears this bit. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 114 1 when the temperature control unit restarts itself after a software or hardware failure. If the temperature control unit is turned on by the power switch the bit is always reset to 0. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 115: Str - Stirrer

    Reply Description “CSM – Agilent ChemStation Mode” on page 98 for interpreting the reply when using the CSM command. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 116: Tem - Cell Temperature

    (–999.99 | 999.99) regardless of the selected units. The out of limits result could be caused by a defective sensor. N O T E Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 117: Tra - Trigger Acknowledge

    (remote control connector pin 4) condition from the temperature control unit from a external device. The TRA instruction clears the TRIGGER bit in the temperature control unit status byte. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 118: Trg - Trigger Out

    Using the command TRG LOW sets: OUTTRIG line (pin 9) to low (L) (0 V). Reply Description “CSM – Agilent ChemStation Mode” on page 98 for interpreting the reply when using the CSM command. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 119: Tst - Test

    1 set_temperature [-][dd]d.dd 2 free 3 free 4 stirrer speed [dd]dd 5 free 6 free 7 free 8 free 9 over_temperature_counter [dddd]d 10 free 11 gpib_address [d]d (1 -31) 12 error_code ddd Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 120 Over_temperature_counter records the amount of time, in 10 minute parts, that the Peltier N O T E element is used above 70°C. Store_counter is the record of write cycles to the EEPROM Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 121: Electronics Of The Temperature Control Accessory

    Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual Electronics of the Temperature Control Accessory Electronics Overview Power Supply Microcontroller and Peripherals Keyboard Display Electronic Power Amplifier Sensor Electronic This chapter describes the electronics system of the temperature control accessory.

  • Page 122: Electronics Overview

    17) are supplied. With the Agilent 89090 single cell temperature station (cell holder), only channel 1 is used. The electronics for a second channel (channel 0) is built in for future use. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 123 An external sensor (NTC) can be used to measure the cell temperature or any other remote temperature. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 124: Power Supply

    You access the line fuse (primary fuse) in the line filter from the rear of the instrument after removing the power cord. T1000 mA for 110 VAC operation (part number 2110-0007) T500 mA for 220 VAC operation (part number 2110-0202) Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 125: Internal Fuses

    As soon as the unregulated +30 V line is higher than +20 V and the unregulated +5 V line is higher than 4.8 V, a reset is issued to the microcontroller so that it starts operation in a defined state. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 126: Gnd, Agnd, Pgnd, Sgnd, Shield

    H-bridges. Stirrer ground (SGND) is an extra ground line which is used for the magnetic stirrer, because the coils of the magnetic stirrer induce spikes on the ground line. All ground lines are connected in the power supply. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 127: Microcontroller And Peripherals

    16 bit data/address bus, a serial bus, pulse width modulated outputs and interrupt control logic. An address decoder is needed to separate the addresses from the 16 bit data/address bus. Figure 19 Microcontroller and Peripherals Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 128: Prom Card

    The switch enable lines SWE0/1 and switch control lines SWC0/1 are directly controlled from the microcontroller. SWE1 is an enabled line for the power amplifiers of channel 1 (SWE0 is used for channel 0). Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 129: Lines To Sensor Electronic

    The watchdog (WO) line becomes active, if the watchdog circuit is not accessed in regular intervals by the firmware or if it is accessed too often (program running in loop). Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 130: Keyboard Display Electronic

    Keyboard Display Electronic Keyboard Display Electronic Figure 20 Keyboard and Display Electronic Keyboard KB0 and KB1 lines are driven from the microcontroller and KB2, KB3, ... to KB9 lines are sensed. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 131: Leds

    ASCII are used to address the internal registers. Brightness of the display is controlled by the microcontroller. The reset line brings the internal logic back to a defined state. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 132: Power Amplifier

    (actual). The result of the comparison is used to drive the current switch. An internal protection mechanism avoids destruction of the switch control circuit due to overcurrent. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 133: Switch

    The power amplifier (PASENS1) line delivers a voltage to the ADC of the sensor electronic. This signal is used for diagnostic purposes of the power amplifier and the H-bridge (PASENS0 is used for channel 0). Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 134: Sensor Electronic

    Electronics of the Temperature Control Accessory Sensor Electronic Sensor Electronic The sensor electronic is the interface between the internal or external sensor and the microcontroller. Figure 22 Sensor Electronic Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 135: Current Source

    Agilent 89090 single cell temperature station (cell holder). R is presently not available. R through R are calibration int0 cal1 cal5 resistors. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 136: Measuring Principle

    Measuring the calibration resistors starts with the highest for R (30 kOhm caln resistance = 0°C) and steps down until it finds the appropriate R range caln compared with the actual value on R Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 137: +5 V Regulator, Voltage Divider

    The other 5 inputs (PASENS1, PASENS0, ICTRL1, ICTRL0 and APWRCHK) are used for diagnostic purposes during self-test. The diagnostic checks which are done with the help of theses lines are described in Chapter “Troubleshooting”, see the appropriate error messages. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 138 Electronics of the Temperature Control Accessory Sensor Electronic Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 139: Cables And Connectors

    Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual Cables and Connectors Cables Connectors Agilent Technologies...

  • Page 140: Cables

    +30 V STIRRERA STIRRERC PELTIER2B PELTIER2B PELTIER2A SCODE0* Sensor Rint1 Sensor Rint2 PELTIER1A SCODE0* = Low and SCODE1 = High select the cell holder (-) sign indicates negative true logic Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 141 PELTIER1B PELTIER1B SGND STIRRERB STIRRERD PELTIER2B PELTIER2A PELTIER2A SCODE1* 24AGND 25AGND * SCODE0 SCODE0* = Low and SCODE1 = High select the cell holder (-) sign indicates negative true logic Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 142: Connectors

    The following tables give the functions of each pin of the connectors on the panels of the temperature control unit. Table 16 GPIB Connector (J190) Function Function Function DI01 DI02 DI03 DI04 Housing Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 143 Data/Address 3 Address 13 Address 4 Address 14 Data/Address 4 Card Detect Address 3 Data/Address 5 +5 V Address 2 Data/Address 6 +5 V (-) sign indicates negative true logic Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 144 Cell Holder Connector (J120) Function Function PELTIER1A PELTIER1A PELTIER1A PELTIER1B PELTIER1B PELTIER1B * SCODE0 = Low and SCODE1 = High select the cell holder (-) sign indicates negative true logic Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 145 AC lines for S15 V supply (> 16 VAC) AC lines for +5 V supply (> 6.5 VAC) AC lines for ±15 V supply (> 14 VAC) AGND (GND of ±15 V supply) Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 146 Data/Address 3 Address 1 Data/Address 4 Address 2 Data/Address 5 Address 3 +5 V Data/Address 6 Address 4 Ready LED Data/Address 7 Address 5 (-) sign indicates negative true logic Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 147: Troubleshooting

    Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual Troubleshooting General Description Basic Failures Error Messages This section describes troubleshooting of the temperature controller and is intended for the use by trained service personnel only. Troubleshooting is divided into three parts: 1 General description.

  • Page 148: General Description

    Press E to continue the self-test or C to stop the NTER LEAR self-test. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 149 Troubleshooting General Description The self-test includes all diagnostic functions and you may receive all error messages, described in this handbook. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 150: Basic Failures

    Display Partly Not Functioning Action: 1 Exchange display assembly. Action: LEDs Not Functioning 1 Check or exchange front panel cable. 2 Exchange front panel assembly. 3 Exchange temperature controller board. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 151 If the whole keyboard or some keys do not function: 1 Check or exchange front panel cable. 2 Exchange temperature controller board. If only one key does not function: 1 Exchange front panel assembly. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 152: Error Messages

    ERR 114 Sensor electronic failure sensor (gain amplifier) electronic, power amplifier ERR 115 Power amplifier failure power amplifier (channel 0) ERR 116 Power amplifier failure power amplifier (channel 1) Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 153 Internal sensor failed (channel 0) ERR 132 Internal sensor failed (channel 1) ERR 133 Cell holder failure (Peltier of channel 0) ERR 134 Cell holder failure (Peltier of channel 1) Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 154 1 Check fuses F861 and F862. 2 Measure voltages on power supply connector (J840). If all volages are not present, exchange the inner cabinet assembly. 3 Exchange temperature controller board. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 155 This error turns the power amplifiers and the sensor electronic off automatically. Checked: After turning on, during self-test. Action: 1 Exchange temperature controller board. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 156 PADIR02 lines. The processor checks the PASENS0 line for a correct voltage drop on Q and the current sensor. For the check of the PASENS1 line (channel 1), the PADIR11 and PADIR12 lines are used to shorten the H-bridge. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 157 ERR 121 relates to H-bridge of channel 0, whereas ERR 122 relates to H-bridge of channel 1. Checked: During self-test. Action: 1 Exchange temperature controller board. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 158 The processor automatically determines if the external sensor is connected, using the SCODE_EXT line of the external sensor connector (see Chapter 11, “Cables and Connectors”, J140). Checked: During self-test. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 159 SCODE0 and SCODE1 lines of the cell holder connector (see J120). ERR 131 relates to internal sensor of channel 0 (presently not used), whereas ERR 132 relates to internal sensor of channel 1 (cell holder). Checked: During self-test. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 160 (see J120). ERR 133 relates to Peltier element of channel 0 (presently not used), whereas ERR 134 relates to Peltier element of channel 1 (cell holder). Checked: During self-test. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 161 This indicates GPIB hardware or firmware error 1 Turn off the temperature controller. 2 Turn on again, if error continues turn off. 3 Exchange temperature controller board. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 162 Troubleshooting Error Messages Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 163: Identifying And Replacing Parts

    Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual Identifying and Replacing Parts Parts Identification Changing Parts Use the section “Parts Identification” on page 165 to identify parts and find part numbers. The section “Changing Parts” on page 167 gives some replacement procedures.
  • Page 164 Identifying and Replacing Parts Figure 23 Overall Diagram of the Temperature Control Unit Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 165: Parts Identification

    Screw M3, 8 mm long 0515-1430 Front panel cable 5062-2412 Pushbutton 5041-1203 Fan assembly Tapping screw M4, 20 mm long Lock washer for M4 2190-0409 Ferrite bar 9170-1392 Metal bar Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 166 Agilent Technologies Part Number External temperature sensor 89090-82100 ell holder Cover, cell holder 89090-45203 Stainless-steel cover Lever 89054-40001 Cable, cell holder 89090-61601 Adapter, Cell Holder (KEL-F) 5021-1870 Stirring bar 9301-1161 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 167: Changing Parts

    4 To replace the board, place it in the inner cabinet as shown in Figure Move it in the direction, indicated by arrow 1, and then move it in the direction indicated by arrow 2. Figure 24 Positioning Temperature Controller Board Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 168: Changing Inner Cabinet Assembly

    The transformer and line-power socket are part of the inner cabinet assembly. To change the inner cabinet assembly you have to remove the following items: Power switch Temperature controller board Fan assembly Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 169: Changing Cell Holder Cable

    10 Place the spare wires 5 of the cell holder cable in the gap between connector and housing. 11 Replace the ground plate of the cell holder and tighten the three screws. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 170 Identifying and Replacing Parts Changing Parts Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 171 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual Safety Information Safety Information Radio Interference Agilent Technologies on Internet Agilent Technologies...

  • Page 172: A Safety Information

    Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuseholders must be avoided. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 173: Safety Symbols

    Eye damage may result from directly viewing light produced by deuterium lamps used in detectors and spectrophotometers. Always turn off the deuterium lamp before opening the lamp door on the instrument. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 174 Zolang de naast het waarschuwingsteken aangegeven aanwijzingen niet volledig begrepen en opgevolgd zijn, dienen geen verdere stappen ondernomen te worden. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 175 Zolang de naast het teken voorzichtig aangegeven aanwijzingen niet volledig begrepen en opgevolgd zijn, dienen geen verdere stappen ondernomen te worden. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 176: Radio Interference

    Test- und Messgeräte Werden Mess- und Testgeräte mit ungeschirmten Kabeln und/oder in offenen Messaufbauten verwendet, so ist vom Betreiber sicherzustellen, dass die Funk-Entstörbestimmungen unter Betriebsbedingungen an seiner Grundstcksgrenze eingehalten werden. Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 177: Agilent Technologies On Internet

    Safety Information Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com/chem Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 178 Safety Information Agilent Technologies on Internet Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...

  • Page 179: Index

    ERR 134, 153, cover, cell holder, STR, TEM, external temperature, Agilent ChemStation mode, TRA, TRG, TST, gripper, ferrule, device clear, fitting, disk, flow cell, display, function heat sink, selecting, setting, value, environment, Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 180 Ready lamp, probe, ready lamp, range, units, remote, remote control, trigger acknowledge, remote mode, 65, Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 182 In This Book This book provides information on how to install the Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer on both a Cerity NDS Professional system (stand-alone) and client/server installation.  Agilent Technologies 2000, 2004 Printed in Germany...

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