Maintenance; Spare Vessel - Thermo Scientific Apreo User's Operation Manual

1. Vent the specimen chamber (the excess LN2 starts to boil).
2. Unclip the Nitrogen vessel from the Vacuum vessel. Lift the
Nitrogen vessel out of the Vacuum vessel by the Safety
pliers placed under the ring on the neck of the Dewar
cylinder.
3. Place the Lid over the Vacuum vessel to seal it from the
atmosphere (fix the clips). Pump the specimen chamber
again. The microscope vacuum remains cleaner than
before, and sample contamination is still reduced.
4. Remove the cap from the Nitrogen vessel and pour out
the excess LN2 into a suitable container.
W A R N I N G !
When the LN2 is removed from the nitrogen vessel, the bottle still remains at a very low temperature!
Baking Vessel
1. Place the Nitrogen vessel onto the stand ready for baking.
2. Place the vessel stand on a suitable heat-resistant surface.
3. Place the vessel onto it and use an Infra-red lamp to bake the base of the bottle. Baking should take place for
approximately 2 hours.
Alternatively, the vessel can be baked in an oven at 90 °C for 2 hours.
4. Allow the vessel to cool down before handling!
Regenerating the Dewar by heat allows removal of condensed contamination and subsequent reuse of the vessel.
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Note
The oven that is used must have a venting system to extract any harmful fumes. Alternatively, it should be baked in a fume
cupboard using an infra-red lamp.
Replacing Vessel
1. Allow the vessel to cool down before handling!
2. Vent the specimen chamber.
3. Unlock two clips holding the Vacuum vessel lid. Remove the lid from the Vacuum vessel.
4. The Nitrogen vessel can be placed in the Vacuum vessel, taking care that the 'O' ring seal on the Vacuum vessel
is secure when joining the two components together. Secure the two components by fixing the clips to the top of
the Nitrogen vessel and locking the clips down.
5. Pump the specimen chamber. The Vacuum vessel is pumped along with the specimen chamber.

Maintenance

• Keep the 'O' rings clean of dust and fiber particles by inspecting the Vacuum vessel main 'O' ring on a regular
basis. If the Vacuum vessel is removed frequently from the specimen chamber, inspect the specimen chamber
'O' ring seal each time.
• Keep the sealing surfaces of the Nitrogen vessel and the Vacuum vessel lid clean and free of dust and fiber
particles.
• Do not use any kind of vacuum grease on the 'O' rings.
• Wipe the outsides of the stainless steel parts to remove fingerprint stains with a lint-free cloth dampened with a
neutral pH soap solution.

Spare Vessel

It is possible to obtain a secondary nitrogen vessel kit, which contains:
• Nitrogen Vessel
• Vessel Stand
• Vessel Plug
C O N F I D E N T I A L – limited rights
Revision A Feb 2018
System Options: CryoCleanerEC
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User Manual 7-33