Related Manuals for Thermo Scientific A2-OK
Summary of Contents for Thermo Scientific A2-OK
- Page 1 Horizontal System Model A2-OK Operating and Maintenance Manual 7219011 Rev. 0 Visit us online to register your warranty www.thermoscientific.com/warranty...
- Page 2 Preface MANUAL NUMBER 7219011 4/13/12 Transfer to Marietta (was 11/2002) ECR/ECN DATE DESCRIPTION Thermo Scientific Horizontal System...
- Page 3 Thermo Fisher Scientific makes no representations or warranties with respect to this manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to the use of this manual. ©2012 Thermo Fisher Scientific. All rights reserved. Horizontal System Thermo Scientific...
- Page 4 Always use the proper protective equipment (clothing, gloves, goggles, etc.) 4 Always dissipate extreme cold or heat and wear protective clothing. 4 Always follow good hygiene practices. 4 Each individual is responsible for his or her own safety. Thermo Scientific Horizontal System...
- Page 5 We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products. Whatever Thermo Scientific products you need or use, we will be happy to discuss your applications. If you are experiencing technical problems, working together, we will help you locate the problem and, chances are, correct it yourself...over the telephone without a service...
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Page 6: Table Of Contents
Replacement Parts ........8-1 Thermo Scientific... -
Page 8: General Information
Table 1-1. Parts List Buffer Chamber Combs 10 well comb, Double Sided, 1.0/1.5mm Thick (12) Super Safe Lid with attached power supply leads Multiple Gel Caster (2) Gel Tray Holder UV Transmissible gel trays (6) Thermo Scientific Horizontal System... -
Page 9: Section 1 General Information
Running Buffer ....Volume 2.3L Footprint ..28cm W x 37.5cm L x 10cm H Horizontal System Thermo Scientific... -
Page 10: Setting Up
Place the gel tray holder in the buffer chamber, add buffer, remove combs, load samples and run the gel. Thermo Scientific Horizontal System... -
Page 11: Preparing The Gel
1 or 2 sets of equal distance samples simultaneously expanding the number of samples per gel that may be run. Repeat for up to 6 gels (3 per external multiple gel caster). Horizontal System Thermo Scientific... -
Page 12: Using The System
DNA mobility and cause band distortion. 3. Carefully remove the comb (or combs) by tapping lightly to loosen, and slowly lifting straight up out of the gel tray to avoid damage to the wells. Thermo Scientific Horizontal System... -
Page 13: Section 3 Using The System
Plug other end of the power cords into appropriate power supply. 6. Turn on power supply (see Recommended Running Conditions). Carefully monitor the gel run to avoid samples running into the path of another set of samples. Horizontal System Thermo Scientific... -
Page 14: Finishing Up
Warning Ethidium bromide is a potential carcinogen. Care in handling the powder and stock solution must be taken. Always wear gloves when handling the powder, solutions and all gels that contain any amount of ethidium bromide. Thermo Scientific Horizontal System... -
Page 16: Technical Tips
One long well and one marker lane. Preparative Wall Comb • See page 33 for details. Wall Comb Multi-Load Comb • F or use with 8 channel pipet. Custom Combs Multi Load Comb Call Technical Services for more information. Thermo Scientific Horizontal System... -
Page 17: Reagent Information
(5mg/ml) is added. The gel would be run with compatible electrophoretic running buffer (1X TBE or 1X TAE) that also contained ethidium bromide. 1 liter of the running buffer would contain 100ul of this 5mg/ml ethidium bromide stock solution. Horizontal System Thermo Scientific... - Page 18 - no recirculation needed for extended run times. TBE buffer reacts with the agarose making smaller pores and a tighter matrix. This reduces broadening of the DNA bands for sharper resolution. Thermo Scientific Horizontal System...
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Page 19: Section 5 Technical Tips
Gel size(cm) 0.25cm 0.5cm 0.75cm 1.0cm A2-OK Agarose Gel Loading Buffer Samples are prepared and combine with gel loading buffer before applying to the prepared gel. Sample buffer usually contains similar components to the running buffer, dyes for visibility, and glycerol to provide some weight to the samples. -
Page 20: Troubleshooting
Gels and appear diffuse in the gel. should be submerged in 3-5mm of buffer to avoid gel dry out, but excess buffer >5mm can cause decreased DNA mobility and band distortion. Thermo Scientific Horizontal System... -
Page 21: Section 6 Troubleshooting
If excessive running buffer is added, the mobility of the DNA decreases and band distortion may result. Excess buffer causes heat to build up and buffer condensation inside the unit may result. Horizontal System Thermo Scientific... - Page 22 Fotador, U.. Simultaneous Use of Standard and Low-Melting Agarose for the Separation and Isolation of DNA by Electrophoresis, BioTechniques, Vol. 10, No. 2, (1991) Boots, S. Gel Electrophoresis of DNA ; Analytical Chemistry, Vol. 61, No. 8, April 15, 1989 Thermo Scientific Horizontal System...
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Page 24: Care And Cleaning
7005 ......4 liter bottle *Rnase Away® is a registered trademark of Molecular BioProducts Thermo Scientific Horizontal System... -
Page 25: Section 7 Care And Cleaning
S–Safe (No effect, except possibly some staining) A–Attacked (Slight attack by, or absorption of, the liquid) (Slight crazing or swelling, but acrylic has retained most of its strength) U–Unsatisfactory (Softened, swollen, slowly dissolved) D–Dissolved (In seven days, or less) Horizontal System Thermo Scientific... - Page 26 Alconox™, and can also be exposed to a mild bleach solution (10:1). In addition, RNAse removal products are also safe for acrylic. Contact Technical Services with any questions. Thermo Scientific Horizontal System...
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Page 28: Optional Equipment
B1A-GK ...Replacement Gaskets (1 pair) Combs Catalog # # of teeth Tooth Width (mm) Thickness (mm) B1A-5C B1A-5D B1A-6 1.5/1.0 B1A-8 1.5 /1.0 B1A-10 1.5 /1.0 B1A-12 1.5 /1.0 B1A-PREP 57/4.8 Thermo Scientific Horizontal System... - Page 30 Section 9 Warranty Information Thermo Scientific Horizontal System...
- Page 31 Section 9 Warranty Information Horizontal System Thermo Scientific...
- Page 32 Thermo Fisher Scientific 401 Millcreek Road Marietta, Ohio 45750 United States www.thermofisher.com...