Thermo Scientific Apreo User's Operation Manual page 39

Integrate
Filter allows cumulative noise reduction by true integration over a specified number (2 or more) of frames. This
process continues until the selected number of frames is reached. It then automatically pauses the imaging.
This can be used as an alternative to slow scanning to obtain high quality images of slightly charging specimens.
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Note
Clicking on the down arrow below the toolbar icon shows menu items Live /
Average (# frames) / Integrate (# frames) / Number of frames, enabling the
user to select the number of averaged or integrated images (depending on the
actually active filter indicated by the icon for the active display). Clicking on
the toolbar icon itself changes the Live / Average / Integrate filter in cycle.
The Number of frames is set and remembered independently for the
Average and Integrate filters. Both the filter and Number of frames are
set and remembered per display, so live and filtered imaging can run at
the same time. Settings are particular for the Reduced Area, Full Frame
and for the Line scan. The Photo / Snapshot function uses the Integrate
and Number of frames pre-set (see the Preferences / Scanning section).
As scanning can take a significantly long period of time, one can restart
scanning from its beginning by pressing the Ctrl + R keys.
Scan Rotation (Shift + F12)
This on-screen tool is activated to rotate the scan field. It has no effect on the stage movements, and is solely a
scan coil/electrode function used to orient the imaging relative to a specimen feature and/or detector direction.
A non-zero scan rotation is indicated by an icon in the Status bar.
Preferences (Ctrl + Alt + S)
Clicking this item opens the Preferences dialog / Scanning item (see further).
Beam menu
opens the Beam menu functions:
SEM Aperture
This item is not available for the user / supervisor accounts. It is intended
for service interventions.
Home SEM Apertures
This function sends the SEM final lens aperture (Automatic Aperture
System – see Chapter 2) to its home position in case of trouble.
Degauss (F8)
This feature triggers the procedure that puts all actually used electron lenses to a normalized state by removing
their hysteresis effects. For a few seconds, while the procedure is running, all live imaging disappears or turn fuzzy.
It then returns.
Use this function with (almost) focused imaging to obtain the most accurate Magnification, Horizontal Field Width
(HFW) and Working Distance (WD) readouts.
Lens Alignment (Shift + F4)
This feature activates / deactivates the final lens alignment mode for the fine alignment of the electron beam only.
The scanning conditions change to the last selected value, the lens modulator turns on, and the alignment cross
appears in its respective position in all imaging displays.
Clicking on & dragging within a display activates a 4-ended arrow cursor and starts the final lens alignment.
Magnification Correction
Applies the Magnification Correction alignment influences on imaging. The
letter "c" is added to the appropriate image databar fields (Mag, HFW),
and the text Magnification Correction is added to the imaging display.
C O N F I D E N T I A L – limited rights
Revision A Feb 2018
Software control: Microscope Control
User Manual 3-13