Thermo Scientific Invitrogen E-Gel G8100 User Manual page 5

Upgrade system firmware ......................................................................................................37
Battery replacement ..............................................................................................................38
Instrument Specifications .......................................................................................................38
Instrument dimensions and specifications ..............................................................................38
Electrical requirements.......................................................................................................39
Environmental requirements................................................................................................39
Appendix C.......................................................................................................................... 40
E-Gel agarose gels ..............................................................................................................40
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Choosing the right gel ............................................................................................................41
Analytical gels ...................................................................................................................41
Gels for preparative gel electrophoresis in Cloning and NGS applications ...................................41
Other available gel types for routine electrophoresis................................................................42
Opening E-Gel cassettes .......................................................................................................42
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Gel Knife ...........................................................................................................................42
Open E-Gel EX and NGS cassettes with a Gel Knife.................................................................42
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Cleaning and storage ..........................................................................................................43
E-Gel Opener ...................................................................................................................43
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Open the E-Gel cassette with an E-Gel
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Cleaning and storage ..........................................................................................................44
E-Gel agarose gel disposal guidelines ..................................................................................44
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Appendix D ......................................................................................................................... 45
Choosing the right DNA ladder ................................................................................................45
Appendix E.......................................................................................................................... 46
Running RNA Samples on E-Gel
Non-denaturing conditions ..................................................................................................46
Denaturing agents ..............................................................................................................46
Denaturing conditions .........................................................................................................46
Appendix F.......................................................................................................................... 47
E-Gel Power Snap Blue-Light Transilluminator ........................................................................47
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Imaging E-Gels on Third Party Gel Imagers ............................................................................47
Nucleic acid stain use in E-Gel
SYBR Safe DNA Gel Stain ...................................................................................................48
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Safety features...................................................................................................................48
Cloning benefits .................................................................................................................48
Disposal............................................................................................................................48
Spectrum ..........................................................................................................................48
Visualization ......................................................................................................................48
SYBR Gold II Gel Stain........................................................................................................49
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Disposal............................................................................................................................49
Spectrum ..........................................................................................................................49
Visualization ......................................................................................................................49
Appendix G ......................................................................................................................... 50
E-G el Power Snap Electroph oresis System User Guide
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Opener .....................................................................44
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EX Agarose Gels .....................................................................46
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agarose gels ............................................................................48
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