Instrument Accuracy - tecan sunrise RC Technical Manual

12. Performance Testing / Quality Control

12.4.2 Instrument Accuracy

Example for a standard instrument
12-10 Technical Manual for sunrise RC, TW, TS, TC, BC, 6F No. T 137 302 Rev No. 1.1
The accuracy of the instrument can be checked using different absorbance level
neutral density filters and a reference spectrophotometer.
The reference spectrophotometer should be specified higher by the factor 10, at
least by the factor 2, than the Absorbance Reader.
Measure the absorbance of the filters using both the instrument and the
spectrophotometer at different wavelengths and compare the results obtained.
Make sure that the instrument is using the accuracy measurement mode.
The inaccuracy using a standard filter should not be greater than:
at 492 nm, 0.000 - 2.000 Abs.: +/- 1.0 % and +/- 0.010 Abs.
at 492 nm, 2.001 - 3.000 Abs.: +/- 1.0 % and +/- 0.010 Abs.
Example
The following neutral density filters were measured at 405 and 492 nm
wavelengths.
Filters used 0.451, 1.199, 1.586 Abs.
Spectro-
Wavelength
photometer
405 0.463
1.199
1.589
492 0.662
1.698
2.253
Tolerances sunrise
+/- 0.015 0.465
+/- 0.022 1.208
+/- 0.026 1.601
+/- 0.017 0.663
+/- 0.027 1.702
+/- 0.044 2.279
Inaccuracy
+ 0.002
+ 0.009
+ 0.012
+ 0.001
+ 0.004
+ 0.026
2004-10