Microplate Shaking - tecan sunrise RC Technical Manual

2. General
2.6.2

Microplate Shaking

2-18 Technical Manual for sunrise RC, TW, TS, TC, BC, 6F No. T 137 302 Rev No. 1.1
With the Normal and Accuracy measurement modes, the optical density is
measured at three positions across the wells and the average measured optical
density value from the three measurements is used as the optical density of the
well.
The accurate measurement cycle should always be used when measuring
The Center measurement mode should be used if the liquid in the microplate
produces a high meniscus, as an incorrect optical density could be obtained if the
optical density is measured at three positions. If an agglutination measurement is
performed, up to 40 measurement positions per well are used.
For more information about setting the measurement mode,
see 4.3.5 Define Measurement Mode.
sunrise
The is able to shake the microplate before it is measured. Use external
software (for example: Magellan) to set the shaking modes.
The microplate can also be shaken between each of the kinetic measurement
cycles.
WHEN USING A 96 WELL PLATE IN HIGH SHAKE MODE, SPILLAGE
MAY OCCUR IF THE WELLS ARE FILLED WITH MORE THAN 300 µl.
The shaking widths and frequencies for the 4
follows:
Shake Mode Shake Width
HIGH 2.8 mm
NORMAL 4.4 mm
LOW 4.4 mm
WIDE 14.2 mm
Note
high optical densities.
Note
WARNING
sunrise
Shake Frequency
12.3 Hz
9.2 Hz
7.8 Hz
2 Hz
shake modes are as
2004-10