Follow These General Recommendations When Performing A Uplc Analysis - Waters ACQUITY UPLC H-Class Series System Manual

2.1.1 Follow these general recommendations when performing a
UPLC analysis
Select appropriate solutions
• Use high-quality solvents, buffers, and additives (HPLC or MS grade).
• Keep concentrated stock solutions, to use when preparing working solutions.
• Start gradients that include an organic component (0.1%, for example) to provide more
consistent and predictable gradient formation than when you start with 0% organic.
• Ensure that gradients include an organic component (0.1%, for example) to start, to provide
more consistent and predictable.
Set up the system properly
• When installing or removing a column, always hold the active preheater's reusable
compression fitting in place while gripping the column with the column gripping tool. Rotate
the column or optional in-line filter to install or remove it.
• Always use solvent filters on tubing lines in solvent bottles.
• Use the Load Ahead option when you desire a shorter cycle time.
Prime properly
• Prime solvent lines during system start-up.
• Keep the seal wash line and all solvent lines primed.
Manage waste properly
• Do not block the degasser vent line; trim the tubing, if necessary.
• Place the waste container below the system stack to permit gravity flow.
• Ensure that the waste tube does not crimp or bend. A crimp or bend can impede flow to the
waste container.
• Ensure that the exit of the waste tube is not immersed in waste solvent.
• If necessary, shorten the tube so that no portion of it drops below the top of the waste
container.
Note:
To avoid spills, empty the waste container at regular intervals.
Use care when using buffers
• Do not use buffers in the wash solvent line.
• Do not top off buffers, which can promote microbial growth.
• Filter buffers with a 0.2-µm filter membrane.
April 4, 2018, 715005702 Rev. A
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