Priming The Pump - Agilent Technologies 1290 User Manual

4
Using the Pump

Priming the Pump

Priming the Pump

When the solvents have been exchanged or the pumping system has been
turned off for a certain time (for example, overnight) oxygen will
re- diffuse into the solvent channel between the solvent reservoir, vacuum
degassing unit (when available in the system) and the pump. Solvents
containing volatile ingredients will slightly lose these. Therefore priming of
the pumping system is required before starting an application.
1 Initiate a purge in the controlling software with a Purge flow set to 3 –
2 Flush all tubes with at least 30 ml of solvent.
Table 6 Choice of Priming Solvents for Different Purposes
Activity
After an installation
When switching between reverse phase and
normal phase (both times)
After an installation
To clean the system when using buffers
After a solvent change
Before turning off system for an extended
period of time
The pump should never be used for priming empty tubings (never let the pump run dry). Use
N O T E
a syringe to draw enough solvent for completely filling the tubings to the pump inlet before
continuing to prime with the pump.
68
5 ml/min per channel.
Solvent
Isopropanol
Isopropanol
Ethanol or Methanol
Bidistilled water
Bidistilled water
Organic or 10 %
isopropanol in water
Comments
Best solvent to flush air out of the system
Isopropanol is miscible with both normal phase
and reverse phase solvents.
Alternative to Isopropanol (second choice) if no
Isopropanol is available
Best solvent to re-dissolve buffer cristals
Best solvent to re-dissolve buffer cristals
1290 Infinity Quaternary Pump User Manual