Dna Sample Preparation - Agilent Technologies 2200 TapeStation User Manual

DNA Sample Preparation

Parts required p/n
5067-5373
5067-5374
1 Prepare Ladder
2 Prepare sample
The TapeStation will load from a minimum of 3 µL.
N O T E
The sample must be placed at the bottom of the tube or well for sample loading and must
not contain any air bubbles.
The Ladder must be used with every instrument run and selected to load into the first
N O T E
available ScreenTape lane.
Only Agilent Technologies provided ladder and loading buffers must be used with the
N O T E
Agilent 2200 TapeStation ScreenPlex System.
A final concentration of 1 – 30 ng/fragment double stranded DNA should be used, not
N O T E
exceeding 75 ng in total, to achieve the maximal performance.
2200 TapeStation
Description
ScreenTape DS12, 7 /box
ScreenTape DS12 Reagents (DS12 Ladder & DS12 Loading Buffer)
a Aliquot a minimum of 3 µL DS12 Ladder
a Mix 6 µL DS12 loading buffer
Spin down to position the sample at the bottom of the tube/well.
Using the 2200 TapeStation System

DNA Sample Preparation

into the first tube/well.
with 2 µL DNA sample by vortex for 5 s.
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