Agilent Technologies 2200 TapeStation User Manual page 40

4
Using the 2200 TapeStation System
How to prepare your samples
• When pipetting sample buffer, ensure that excess buffer droplets are removed from the
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• When pipetting small volumes ensure that no sample remains within the tip.
• When adding sample buffer to sample, please ensure that they are mixed correctly.
• For best results ensure that all reagents are allowed to equilibrate to room temperature
For successful loading, the sample solution must be placed at the bottom of the tube or
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well without any air-bubbles. The 2200 TapeStation will load a sample from a minimum of
3 µL onto ScreenTape.
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tip before transfer to the sample tubes. Care must be taken due to viscosity of Sample
Buffers.
To achieve this, gently mix several times with additional pipetting, then cap the tubes,
mix the samples using a vortex mixer for 5 s and briefly centrifuge to collect the
contents at the base of the tubes. Improper mixing can lead to quantification errors.
for 30 minutes prior to use.
2200 TapeStation