Screenplex Software - Agilent Technologies 2200 TapeStation User Manual

ScreenPlex Software

Table 7
Issue
Upon result launch, a
message states that
the software failed to
map a suitable top
and bottom marker.
Upon result launch, a
message states that
the software failed to
map detected peaks
with expected
controls.
Unidentified peaks
have been detected
in the sample.
Re-running a sample
that has been left in
the 2200 TapeStation
has provided a
different result to the
previous run.
A completely blank
lane has appeared in
the results.
All markers and
sample bands within
a lane appear very
faint.
2200 TapeStation

ScreenPlex Software

Probable cause
There may be an
error in the ladder
lane – the bands are
too faint or the lane
is blank.
There may be an
error in the ladder
lane – the ladder has
too many or too few
bands than expected.
There may be the
presence of an
unknown fragment in
the sample or a
particle has adhered
to the outside of
ScreenTape.
The samples were
not prepared
optimally.
There may be a
bubble in the sample.
There may be a
bubble in the sample.
ScreenPlex Software
Solution
Perform the entire run, including samples, on a
new piece of ScreenTape.
Perform the entire run, including samples, on a
new piece of ScreenTape.
Freshly prepare the sample again and re-run on a
new piece of ScreenTape.
Samples should be mixed with loading buffer,
then vortexed for 5 seconds and spun down
before placing in the 2200 TapeStation.
Briefly centrifuge the sample to remove any
bubbles and re-run on a new piece of ScreenTape.
If the problem persists, please contact Agilent
Technologies.
Briefly centrifuge the sample to remove any
bubbles and re-run on a new piece of ScreenTape.
If the problem persists, please contact Agilent
Technologies.
6
Troubleshooting
55