Agilent Technologies 2200 TapeStation User Manual page 37

• The review pane is accessed through Review in the top right hand corner of
• Right clicking on a selected peak within a lane provides the option of
• Right clicking on an unselected fragment within a lane provides the option
• Right clicking in any area of a lane that has been manually altered, and
2200 TapeStation
annotated with a warning sign on the image. If the internal control is
undetected, a warning sign will illustrate the expected position on the gel
image.
the ScreenPlex Result Viewer. Multiple test tabs are located at the top of the
gel image and a comments box is located at the bottom. Any changes made
in the review window should be described in the comments box. These
comments will then appear in the final results file. Analysis changes can
only be made from the review window. Any changes made in review mode
will always be annotated with an m (for manual) at the top of the lane.
deselecting that peak.
•
If deselecting the top band, annotated as the top marker, or the bottom
band, annotated as the bottom marker, the markers will be reassigned.
The software will always assume that the lowest molecular weight
fragment detected is the bottom marker and that the greatest molecular
weight fragment detected is the top marker. Deselecting these annotated
markers so that they are no longer detected will move the position of the
markers.
•
Once deselected, the peak will be annotated with an empty triangle as
notification that an automatically selected peak has been manually
deselected. The top of the lane will now display the letter m.
of adding that peak.
•
If adding a peak above the annotated top marker, or below the annotated
bottom marker, the markers will be reassigned. The software will always
assume that the lowest molecular weight fragment detected is the bottom
marker and that the greatest molecular weight fragment detected is the
top marker. Selecting bands above or below the annotated markers will
move the position of the automatically annotated markers.
• Once selected, the peak will be annotated with a letter m along with an
open arrow (marker) or a grey triangle as notification that a peak that
was not present at result launch has been manually selected. The top of
the lane will now display the letter m.
therefore contains an m at the top, will allow the ability to reset that lane.
This will remove all manual changes and the lane will revert to an
automatically analysed state.
Using the 2200 TapeStation System
Operating Procedure
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