Agilent Technologies 2200 User Manual
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Agilent Technologies 2200 User Manual

Agilent Technologies 2200 User Manual

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  • Page 1 Agilent 2200 TapeStation System User Manual Agilent Technologies...
  • Page 2 1987) or any equivalent agency regulation or contract clause. Use, duplication or dis- closure of Software is subject to Agilent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will Agilent 2200 TapeStation System - User Manual...

  • Page 3: Table Of Contents

    Intended Use of the 2200 TapeStation System Performance Limitations of Use Additional Components Required by the User Operating Procedure How to prepare your samples Good Measurement Practices for Analysing DNA on the Agilent 2200 TapeStation DNA Sample Preparation RNA Sample Preparation Protein Sample Preparation...

  • Page 4: Agilent 2200 Tapestation System - User Manual

    Contents 6 Appendix Limited Use Label License Sound Emission Waste Electrical and Electronic Equipment Directive Technical Service Agilent 2200 TapeStation System - User Manual...

  • Page 5: Introduction To The 2200 Tapestation System

    Agilent 2200 TapeStation System - User Manual Introduction to the 2200 TapeStation System Overview of the System ScreenTape Device Architecture Agilent 2200 TapeStation Instrument Components This chapter gives an introduction to the system Agilent Technologies...

  • Page 6: Overview Of The System

    (2200 TapeStation Controller Software, and Agilent TapeStation Analysis Software). The system is very straightforward to use, simply place the sample tubes and ScreenTape device in to the 2200 TapeStation instrument and let it load, separate, image, analyze, and present the results.
  • Page 7 ScreenTape device The information is unique to each ScreenTape device. This includes: product details: ScreenTape assay type, product expiry date and a unique serial number. Figure 1 Example: P200 ScreenTape device Agilent 2200 TapeStation System - User Manual...

  • Page 8: Agilent 2200 Tapestation Instrument Components

    Introduction to the 2200 TapeStation System Overview of the System Agilent 2200 TapeStation Instrument Components Lid: The 2200 TapeStation instrument lid must be closed each time the 2200 TapeStation Controller Software is initialized, and whilst the instrument is in operation. LED: The LED will illuminate once the instrument is on.
  • Page 9 Introduction to the 2200 TapeStation System Overview of the System Figure 2 2200 TapeStation instrument (front view) On/off switch Power-cable socket USB socket Figure 3 2200 TapeStation instrument (back view) Agilent 2200 TapeStation System - User Manual...
  • Page 10 Introduction to the 2200 TapeStation System Overview of the System Agilent 2200 TapeStation System - User Manual...

  • Page 11: Specifications

    Agilent 2200 TapeStation System - User Manual Specifications Technical Specifications ScreenTape Specifications This chapter provides information on specifications. Agilent Technologies...

  • Page 12: Technical Specifications

    14 – 30 °C (57 – 86 °F) for RNA and High Sensitivity RNA ScreenTape assays 10 – 33 °C (50 – 91 °F) for P200 ScreenTape assay Instrument operating temperature may be higher than ambient lab temperature, especially after prolonged use. Agilent 2200 TapeStation System - User Manual...
  • Page 13 Check that any air conditioning is functioning. • If the quoted current temperature is below the specified range, please allow the instrument to equilibrate to the ambient temperature, and avoid using in a cooled area. Agilent 2200 TapeStation System - User Manual...

  • Page 14: Screentape Specifications

    96 samples: ≈100 min Samples per consumable Sample volume required 1 µL Kit stability 4 months Kit size 112 samples signal-to-noise >3 (single peak) Sizing Accuracy for electronic ladder: ±20% Measured against 2100 Bioanalyzer system Agilent 2200 TapeStation System - User Manual...
  • Page 15 96 samples: ≈100 min Samples per consumable Sample volume required 2 µL Kit stability 4 months Kit size 112 samples signal-to-noise >3 (single peak) Sizing Accuracy for electronic ladder: ±20% Measured against 2100 Bioanalyzer system Agilent 2200 TapeStation System - User Manual...
  • Page 16 1 µL Kit stability 4 months Kit size 112 samples/box signal-to-noise >3 (single peak) Determined using the D5000 Ladder as sample Accuracy for run using single ScreenTape device Measured against 2100 Bioanalyzer system Agilent 2200 TapeStation System - User Manual...
  • Page 17 Kit stability 4 months Kit size 112 samples/box signal-to-noise >3 (single peak) Determined using the High Sensitivity D5000 Ladder as sample Accuracy for run using single ScreenTape device Measured against 2100 Bioanalyzer system Agilent 2200 TapeStation System - User Manual...
  • Page 18 1 ladder + 15 samples Sample Volume Required 1 µL Shelf Life 4 months Box/Kit size 112 samples/box Determined using the Genomic DNA Ladder as sample Average result from various genomic DNA sample types Agilent 2200 TapeStation System - User Manual...
  • Page 19 96 samples < 100 min Samples per consumable Sample volume required (µL) Kit Stability 4 months Kit Size 112 samples For total RNA samples Within a ScreenTape Signal/noise >3 in water and TE device Agilent 2200 TapeStation System - User Manual...
  • Page 20 96 samples < 180 min Samples per consumable Sample volume required (µL) Kit Stability 4 months Kit Size 112 samples For total RNA samples Within a ScreenTape Signal/noise >3 in water and TE device Agilent 2200 TapeStation System - User Manual...
  • Page 21 Physical Specification Sample volume needed 2 µL Analysis Time 16 samples: <15 min Samples/consumable Kit Size 112 Samples Kit Stability 4 months determined using P200 Ladder as sample signal :noise ratio > 3 Agilent 2200 TapeStation System - User Manual...
  • Page 22 Specifications ScreenTape Specifications Agilent 2200 TapeStation System - User Manual...

  • Page 23: Installing The System

    Agilent 2200 TapeStation System - User Manual Installing the System Unpacking the System Contents of the 2200 TapeStation System Installing the System Software Installation Agilent 2200 TapeStation System Set Up This chapter gives information about how to install the system.

  • Page 24: Unpacking The System

    Prerequisites conducted by an Agilent engineer. Instrument and software familiarization should be completed before use. Do not attempt to use the 2200 TapeStation instrument until you have read the accompanying Site and Safety Manual. Condensation within the instrument C A U T I O N Condensation will damage the system electronics.
  • Page 25 Installing the System Unpacking the System 1 Remove the 2200 TapeStation instrument from the packaging and place on a clean, dry, flat surface. 2 Allow the 2200 TapeStation instrument to acclimatize to the ambient temperature of the operating environment. 3 Remove the label covering the ScreenTape device holder, as shown in the image below.

  • Page 26: Contents Of The 2200 Tapestation System

    • 2200 TapeStation system (G2964AA) or instrument • 2200 TapeStation Nucleic Acid system (G2965AA) 2200 TapeStation 1x CD The software is required to drive the 2200 TapeStation instrument and visualize Software Setup Disc the ScreenTape device analysis Laptop 1x Laptop...
  • Page 27 Part Number Name Color Amount 5067-5582 D1000 ScreenTape 7 ScreenTape devices 5067-5583 D1000 Reagents 2 vials • D1000 Ladder 10 µL • D1000 Sample Buffer 400 µL 5067-5586 D1000 Ladder 1 vial, 10 µL Agilent 2200 TapeStation System - User Manual...
  • Page 28 Kit Components (Genomic DNA ScreenTape Assay) Part Number Name Color Amount 5067-5365 Genomic DNA ScreenTape 7 ScreenTape devices 5067-5366 Genomic DNA Reagents 2 vials • Genomic DNA Ladder 25 µL • Genomic DNA Sample Buffer 1350 µL Agilent 2200 TapeStation System - User Manual...
  • Page 29 350 µL • P200 Reducing Sample Buffer 550 µL • P200 pH Buffer 1000 µL clear • P200 Non-Reducing Sample Buffer 550 µL • P200 Markers (pre-stained) 270 µL • P200 Ladder 40 µL Agilent 2200 TapeStation System - User Manual...

  • Page 30: Installing The System

    Installing the System Installing the System Software Installation The software for your Agilent 2200 TapeStation system is preinstalled on the system laptop. For updates, or if you have to change the laptop, you may download the latest version of the N O T E software from the update server http://www.agilent.com/genomics/tapestation.

  • Page 31: Agilent 2200 Tapestation System Set Up

    If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. For general safety information, please refer to the 2200 TapeStation System - Site and N O T E Safety Manual.
  • Page 32 Continue Anyway. A window appears, indicating that the hardware has been successfully installed. The 2200 TapeStation system will function. As there is more than one driver that can be detected and installed, you may need to follow N O T E these steps more than once.

  • Page 33: Using The 2200 Tapestation System

    Empty Tip Buckets How to Use the TapeStation Analysis Software Shutdown and Restarting Procedure How to prepare your samples Good Measurement Practices for Analysing DNA on the Agilent 2200 TapeStation DNA Sample Preparation Sample Preparation D1000 ScreenTape Assay Sample Preparation High Sensitivity D1000 ScreenTape Assay...
  • Page 34 Using the 2200 TapeStation System Installing the System Protein Sample Preparation Sample Preparation P200 ScreenTape Assay This chapter explains the intended use of the 2200 TapeStation system. Agilent 2200 TapeStation System - User Manual...

  • Page 35: Intended Use Of The 2200 Tapestation System

    Using the 2200 TapeStation System Intended Use of the 2200 TapeStation System Intended Use of the 2200 TapeStation System The 2200 TapeStation system carries out electrophoretic separation of Nucleic Acids and proteins. The system detects: • Fluorescently stained double stranded DNA including genomic DNA •...

  • Page 36: Performance Limitations Of Use

    Using the 2200 TapeStation System Performance Limitations of Use Performance Limitations of Use The 2200 TapeStation system can analyze a maximum of 16 samples at any one time, more samples can be run using a 96- well plate and multiple ScreenTape devices.

  • Page 37: Additional Components Required By The User

    Mixing recommendations N O T E • 2200 TapeStation instruments are supplied with an optional IKA MS3 vortexer which includes a 96-well plate adaptor suitable for both 96-well PCR plates and 8-way strips. • This vortexer is recommended for use with all ScreenTape assays with the exception of the P200 ScreenTape assay which should be manually vortexed.

  • Page 38: Operating Procedure

    1 Double click the 2200 TapeStation Controller Software icon on the desktop and follow the instructions on the screen. Always ensure you are using the most up-to-date 2200 TapeStation Controller Software. N O T E Please check for the latest version.
  • Page 39 If any used tips are left in the tip-buckets, a pop-up window will ask for the discarded tips to N O T E be removed. The 2200 TapeStation instrument will not run until all the tip buckets are empty. A Loading tip transfer tool (G2964-60000) is available.
  • Page 40 N O T E bend or flex ScreenTape device and store in the provided packaging at the recommended temperature when not in use. The 2200 TapeStation instrument will not recognize the ScreenTape device if inserted N O T E incorrectly.
  • Page 41 Using the 2200 TapeStation System Operating Procedure Damage to the 2200 TapeStation instrument and impact on performance C A U T I O N ➔ Ensure the lids have been removed from the sample tubes. 9 Select the tubes or wells you wish to run by clicking and dragging the mouse over the sample locations in the user interface.
  • Page 42 • Clear All Selections - this will clear ladder well and all sample wells selected • Clear Last Selection - this will only clear the last samples to be highlighted Pressing Escape on the keyboard will also cancel the current selections. N O T E Agilent 2200 TapeStation System - User Manual...

  • Page 43: Add Experiment Notes

    Using the 2200 TapeStation System Operating Procedure Add Experiment Notes 1 If required, notes can be manually entered into the software before the instrument is started. Agilent 2200 TapeStation System - User Manual...

  • Page 44: Describe Samples

    Describe Samples 1 Sample descriptions can be manually entered into the software before the instrument is started and whilst the 2200 TapeStation instrument is operating, before TapeStation Analysis Software is launched. Sample data can be copied and pasted from an Excel table.
  • Page 45 In the 96-well plate sample selection screen the panel labeled LDR is not available for N O T E selection. All information entered in the 2200 TapeStation Controller Software will appear in the N O T E analysed results. Agilent 2200 TapeStation System - User Manual...

  • Page 46: Start The Run

    3 Close the lid. Lifting the lid of the 2200 TapeStation instrument after this time will abort the experimental N O T E run.

  • Page 47: Running System

    Abort the Run 1 If, for any reason, you wish to abort an experiment, click the abort button on the pop- up controller. The 2200 TapeStation Controller Software will ask: a If you want to reset the instrument to begin another experiment –...

  • Page 48: Empty Tip Buckets

    For further information please refer to the software help. N O T E This can be accessed by selecting the question mark (?) button in the top right hand corner of the TapeStation Analysis Software. Agilent 2200 TapeStation System - User Manual...

  • Page 49: Shutdown And Restarting Procedure

    The 2200 TapeStation Controller Software, 2200 TapeStation instrument and laptop should N O T E be shut down when not in use (preferably at the end of every working day). Ensure that the 2200 TapeStation system is shut down in the following order: 1 Exit the 2200 TapeStation Controller Software.

  • Page 50: How To Prepare Your Samples

    N O T E well without any air-bubbles; centrifugation of samples after preparation is essential. The 2200 TapeStation instrument will load a sample from a minimum of 3 µL onto the ScreenTape device. Agilent 2200 TapeStation System - User Manual...
  • Page 51 Alternatively the user can choose to run an electronic ladder. This is done by choosing No ladder in the 2200 TapeStation Controller Software electronic ladder menu, then running the instrument as normal. An electronic ladder can then be inserted in the TapeStation Analysis Software.

  • Page 52: Good Measurement Practices For Analysing Dna On The Agilent 2200 Tapestation

    DNA assays on the 2200 TapeStation system, and includes advice on the following topics: • Quantification • Sizing • Molarity • Genomic DNA Assay This Technical Note and all other 2200 User Information can be found on the Agilent website: http://www.agilent.com/genomics/tapestation. Agilent 2200 TapeStation System - User Manual...

  • Page 53: Dna Sample Preparation

    As with samples in 8-way strips, briefly centrifuge after vortexing to collect the contents at the base of the tubes before placing into the 2200 TapeStation instrument. D5000 and High Sensitivity D5000 assays are limited to runs using a single ScreenTape device, and cannot be run from 96-well plates.
  • Page 54 N O T E well without any air-bubbles; centrifugation of samples after preparation is essential. The 2200 TapeStation instrument will load a sample from a minimum of 3 µL onto the ScreenTape device. For best sizing precision and accuracy, the user should run the appropriate ladder with the N O T E samples.

  • Page 55: Sample Preparation D1000 Screentape Assay

    Prepare TapeStation System D1000 Parts required Description 5067-5582 D1000 ScreenTape 1 Launch the 2200 TapeStation Controller Software. 2 Load D1000 ScreenTape device and loading tips into the 2200 TapeStation instrument. Sample Preparation D1000 ScreenTape Assay Parts required Description 5067-5583 D1000 Reagents...

  • Page 56: Sample Preparation High Sensitivity D1000 Screentape Assay

    Prepare TapeStation System HSD1000 Parts required Description 5067-5584 High Sensitivity D1000 ScreenTape 1 Launch the 2200 TapeStation Controller Software. 2 Load High Sensitivity D1000 ScreenTape device and loading tips into the 2200 TapeStation instrument. Sample Preparation High Sensitivity D1000 ScreenTape Assay Parts required...

  • Page 57: Sample Preparation D5000 Screentape Assay

    Prepare TapeStation System D5000 Parts required Description 5067-5588 D5000 ScreenTape 1 Launch the 2200 TapeStation Controller Software. 2 Load single D5000 ScreenTape device and loading tips into the 2200 TapeStation instrument Sample Preparation D5000 ScreenTape Assay Parts required Description 5067-5589...

  • Page 58: Sample Preparation High Sensitivity D5000 Screentape Assay

    Parts required Description 5067-5592 High Sensitivity D5000 ScreenTape 1 Launch the 2200 TapeStation Controller Software. 2 Load single High Sensitivity D5000 ScreenTape device and loading tips into the 2200 TapeStation instrument Sample Preparation High Sensitivity D5000 ScreenTape Assay Parts required...

  • Page 59: Sample Preparation Genomic Dna Screentape Assay

    Prepare TapeStation System gDNA Parts required Description 5067-5365 Genomic DNA ScreenTape 1 Launch the 2200 TapeStation Controller Software. 2 Load Genomic DNA ScreenTape device and loading tips into the 2200 TapeStation instrument. Sample Preparation Genomic DNA ScreenTape Assay Parts required Description 5067-5366...
  • Page 60 Using the 2200 TapeStation System DNA Sample Preparation 7 Load sample plate, tips, ScreenTape device, and Samples into the TapeStation instrument as detailed in operating procedure section. Agilent 2200 TapeStation System - User Manual...

  • Page 61: Rna Sample Preparation

    • To ensure optimal performance of the RNA ScreenTape assays, samples should be analyzed using the 2200 TapeStation instrument within 3 h of the denaturation step. Beyond 3 h denatured samples should be stored on ice or in a suitable freezable sample block.
  • Page 62 Mixing recommendations N O T E • 2200 TapeStation instruments are supplied with an optional IKA MS3 vortexer which includes a 96-well plate adaptor suitable for both 96-well PCR plates and 8-way strips. • This vortexer is recommended for use with all ScreenTape assays with the exception of the P200 ScreenTape assay which should be manually vortexed.

  • Page 63: Sample Preparation Rna Screentape Assay

    Description 5067-5576 RNA ScreenTape 1 Launch the 2200 TapeStation Controller Software. 2 Load RNA ScreenTape device and loading tips into the 2200 TapeStation instrument. 3 Select RNA Protocol (Eukaryotic RNA or Prokaryotic RNA) Sample Preparation RNA ScreenTape Assay Parts required...
  • Page 64 Heat ladder and samples at 72 °C (162 °F) for 3 min b Place ladder and samples on ice for 2 min c Spin down to position the sample at the bottom of the tube Agilent 2200 TapeStation System - User Manual...

  • Page 65: Sample Preparation High Sensitivity Rna Screentape Assay

    Parts required Description 5067-5579 High Sensitivity RNA ScreenTape 1 Launch the 2200 TapeStation Controller Software. 2 Load High Sensitivity RNA ScreenTape device and loading tips into the 2200 TapeStation instrument. 3 Select RNA Protocol (Eukaryotic RNA or Prokaryotic RNA) Sample Preparation High Sensitivity RNA ScreenTape Assay...
  • Page 66 Heat ladder and samples at 72 °C (162 °F) for 3 min b Place ladder and samples on ice for 2 min c Spin down to position the sample at the bottom of the tube Agilent 2200 TapeStation System - User Manual...

  • Page 67: Protein Sample Preparation

    N O T E well without any air-bubbles; centrifugation of samples after preparation is essential. The 2200 TapeStation instrument will load a sample from a minimum of 3 µL onto the ScreenTape device. For best sizing precision and accuracy, the user should run the appropriate ladder with the N O T E samples.
  • Page 68 Sample Preparation P200 ScreenTape Assay Prepare TapeStation System P200 Parts required Description 5067-5371 P200 ScreenTape 1 Launch the Agilent 2200 TapeStation Controller Software. 2 Load P200 ScreenTape device and loading tips into the 2200 TapeStation instrument. Sample Preparation P200 ScreenTape Assay Parts required Description...
  • Page 69 N O T E using the P200 reducing buffer. In Ladder mode, selected in the ladder options in the 2200 TapeStation Controller Software, P200 ladder is automatically selected as the first sample. The user can also select to run no ladder, and then to insert an electronic ladder in the TapeStation Analysis Software.
  • Page 70 5 Mix the solution well, and centrifuge to ensure that the sample is at the bottom of the tube, ready for analysis on the 2200 TapeStation system. P200 Marker is formulated with a high percentage of glycerol. Due to the high density of...

  • Page 71: Maintenance

    Agilent 2200 TapeStation System - User Manual Maintenance General Information Changing the Needle Preventative Maintenance Interval TapeStation Test Tape Using the TapeStation Test Tape This chapter describes the maintenance of the TapeStation system. Agilent Technologies...

  • Page 72: General Information

    73, and can be performed by the end user with the aid of a disposable cartridge. Users who wish to check the function of their 2200 TapeStation instrument between PM services can perform a check using the TapeStation Test Tape, as described in “TapeStation Test Tape”...

  • Page 73: Changing The Needle

    Maintenance Changing the Needle Changing the Needle It is important to know which 2200 TapeStation system you have before changing the needle(s), in order to purchase the correct needle cartridge. Table 2 Overview of TapeStation system configuration - needle cartridge...
  • Page 74 Changing the Needle Needle change intervals: • After 7680 lanes (3840 pierces in a Single loading system), the 2200 TapeStation Controller Software will inform the user that a needle change is pending. The word Needle will appear in the bottom of the software inside a yellow box.
  • Page 75 New needle cartridges can be ordered at any time from Agilent Technologies by contacting N O T E your local sales agent. For details on the correct needle cartridge for your 2200 TapeStation instrument, refer to Table 2 on page 73.

  • Page 76: Preventative Maintenance Interval

    Maintenance Preventative Maintenance Interval Preventative Maintenance Interval After 16000 samples, the 2200 TapeStation Controller Software will inform the user that Preventative Maintenance is required. The word Maintenance will appear in the bottom of the software inside a yellow box. The...
  • Page 77 The Preventative Maintenance procedure includes a Needle Change and following PM service both messages will disappear. Figure 9 2200 TapeStation Controller Software indicating both a needle change and a Preventative Maintenance is due. Schedule Preventative Maintenance, do not perform a Needle Change...

  • Page 78: Tapestation Test Tape

    The Agilent TapeStation Test Tape (5067- 5601) is a device designed to be used with the 2200 TapeStation system for hardware diagnosis. The TapeStation Test Tape can be inserted into the 2200 TapeStation instrument to check the performance of electrical and optical components.

  • Page 79: Using The Tapestation Test Tape

    Protect the Test Tape device from excessive force. Do not bend or flex the Test Tape device N O T E and store away from direct light and chemicals in the provided packaging when not in use. Agilent 2200 TapeStation System - User Manual...
  • Page 80 Please save your file to a safe place, where it can be accessed easily if needed for support purposes. Lifting the lid of the 2200 TapeStation instrument after this time will abort the TapeStation N O T E Test Tape run.
  • Page 81 Maintenance TapeStation Test Tape 5 Once the test is complete, the 2200 TapeStation Controller Software will display your TapeStation Test Tape results. Figure 12 2200 TapeStation Controller Software showing TapeStation Test Tape 'Pass' result 6 Both the Electrophoresis test, and Imaging LED functionality test should display green Pass results.
  • Page 82 Maintenance TapeStation Test Tape Agilent 2200 TapeStation System - User Manual...

  • Page 83: Appendix

    Agilent 2200 TapeStation System - User Manual Appendix Limited Use Label License Sound Emission Waste Electrical and Electronic Equipment Directive Technical Service This chapter provides addition information. Agilent Technologies...

  • Page 84: Limited Use Label License

    Molecular Probes, Inc. (a wholly owned subsidiary of Life Technologies Corporation) and Agilent Technologies UK Limited, and the manufacture, use, sale or import of this product is subject to one or more of U.S. Patents and corresponding international equivalents, owned by Molecular Probes, Inc.

  • Page 85: Sound Emission

    This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) Agilent 2200 TapeStation System - User Manual...

  • Page 86: Waste Electrical And Electronic Equipment Directive

    Monitoring and Control Instrumentation product. Do not dispose of in domestic household waste N O T E To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information. Agilent 2200 TapeStation System - User Manual...

  • Page 87: Technical Service

    Appendix Technical Service Technical Service For more information, please contact Agilent Technologies UK Limited www.agilent.com/genomics/contact Agilent 2200 TapeStation System - User Manual...
  • Page 88 WEEE directive test service instrument technical unpacking set up system shutdown limitations of use procedure load software ScreenTape device start up tips sound emission specifications Agilent 2200 TapeStation System - User Manual...
  • Page 89 Index Agilent 2200 TapeStation System - User Manual...
  • Page 90 The manual describes the following: • Introduction to the system • Site requirements and specifications • Installation • Using the system • Maintenance • Product notices © Agilent Technologies 2011-2014, 2015 Printed in Germany 09/2015 *G2964-90003* *G2964-90003* G2964-90003 Rev. C Agilent Technologies...

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