Agilent Technologies 4150 TapeStation system System Manual
  1. Manuals
  2. Brands
  3. Agilent Technologies Manuals
  4. Laboratory Equipment
  5. 4150 TapeStation system
  6. System manual

Agilent Technologies 4150 TapeStation system System Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Agilent 4150 TapeStation
System
System Manual
Agilent Technologies

Advertisement

Table of Contents
loading

Related Manuals for Agilent Technologies 4150 TapeStation system

Summary of Contents for Agilent Technologies 4150 TapeStation system

  • Page 1 Agilent 4150 TapeStation System System Manual Agilent Technologies...
  • Page 2 (June 1987) or any equivalent agency regu- lation or contract clause. Use, duplication or disclosure of Software is subject to Agi- lent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will Agilent 4150 TapeStation System Manual...
  • Page 3 This chapter gives an overview on the three packages within the TapeStation software. 6 Operating Instructions This chapter explains the operation of the 4150 TapeStation system. 7 Good Measurement Practices This chapter summarizes information on good measurement practices for ScreenTape assays.
  • Page 4 In this Guide... 9 Maintenance This chapter describes the maintenance of the 4150 TapeStation system. 10 Troubleshooting This chapter gives an overview about the troubleshooting features of the 4150 TapeStation system. It includes information about application troubleshooting as well. 11 Appendix This chapter contains parts information.

  • Page 5: Table Of Contents

    General Safety Information 2 Legal and Regulatory Sound Emission Waste Electrical and Electronic Equipment (WEEE) Directive 3 Product Description Intended use of the 4150 TapeStation System Overview of the System and ScreenTape Architecture Product Descriptions Physical Specification 4 Installation Site Requirements...
  • Page 6 Mixing Recommendations Centrifugation Recommendations RNA Assays Peak Integration Peak Maxima Versus Average Molecular Weight Sizing and Molarity Sizing Range Quantitative range Maximum Buffer Concentration in Sample RINe Functional Range 8 Instrument Diagnosis Instrument Diagnosis Tests Reporting Agilent 4150 TapeStation System Manual...
  • Page 7 Preparing the Instrument for Transportation Boxing for Transportation Assembling Nest Covers Insertion of the Lower Nest Cover 10 Troubleshooting Software Messages Troubleshooting DNA Applications Troubleshooting RNA Applications Instrument Communication Collecting Troubleshooting Information 11 Appendix Accessories Shipment List Agilent 4150 TapeStation System Manual...
  • Page 8 Contents Agilent 4150 TapeStation System Manual...

  • Page 9: Safety

    Agilent 4150 TapeStation System Manual Safety Symbols Safety Messages General Safety Information This chapter provides information on safety symbols and safety messages. Agilent Technologies...

  • Page 10: Symbols

    TVs and laptops, computer hard drives, credit and ATM cards, data storage media, mechanical watches, hearing aids and speakers. Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields. Agilent 4150 TapeStation System Manual...
  • Page 11 Safety Symbols Table 1 Symbols Indicates a pinching or crushing hazard Indicates a piercing or cutting hazard. Agilent 4150 TapeStation System Manual...

  • Page 12: Safety Messages

    C AU T I O N alerts you to situations that could cause loss of data, or damage of equipment. ➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. Agilent 4150 TapeStation System Manual...

  • Page 13: General Safety Information

    Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. ➔...
  • Page 14 WA R N I N G Personal injury (for example electrical shock, intoxication) ➔ Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel. Agilent 4150 TapeStation System Manual...
  • Page 15 Agilent 4150 TapeStation System Manual Legal and Regulatory Sound Emission Waste Electrical and Electronic Equipment (WEEE) Directive This chapter provides additional information on legal and regulatory aspects. Agilent Technologies...

  • Page 16: Legal And Regulatory Sound Emission

    This product has a sound pressure emission (at the operator position) <70 dB. • Sound Pressure Lp <70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) Agilent 4150 TapeStation System Manual...

  • Page 17: Waste Electrical And Electronic Equipment (Weee) Directive

    The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste. Do not dispose of in domestic household waste N O T E To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information. Agilent 4150 TapeStation System Manual...
  • Page 18 Legal and Regulatory Waste Electrical and Electronic Equipment (WEEE) Directive Agilent 4150 TapeStation System Manual...

  • Page 19: Product Description

    Agilent 4150 TapeStation System Manual Product Description Intended use of the 4150 TapeStation System Overview of the System and ScreenTape Architecture Overview of the System ScreenTape Architecture Product Descriptions Physical Specification This chapter gives an introduction to the instrument and provides information on intended use and instrument specifications.

  • Page 20: Intended Use Of The 4150 Tapestation System

    Intended use of the 4150 TapeStation System Intended use of the 4150 TapeStation System The 4150 TapeStation system is intended to separate nucleic acids by means of electrophoresis. The system detects: • Fluorescently stained double stranded DNA including genomic DNA •...

  • Page 21: Overview Of The System And Screentape Architecture

    (QC) within next-generation sequencing (NGS) and biobank workflows easier and more economical than ever. Figure 1 Overview of the 4150 TapeStation system The Agilent 4150 TapeStation instrument represents an entry-level system ideal for labs handling smaller number of samples. Up to 16 samples can be processed per run.
  • Page 22 Reports can easily be generated and saved in PDF format, or exported for editing in Microsoft Word or Excel. Figure 2 ScreenTape devices for DNA and RNA assays Agilent 4150 TapeStation System Manual...
  • Page 23 Furthermore, the computer specification and installation status of all software components are evaluated. Test reports can easily be generated and saved in PDF format. Figure 3 TapeStation Test Tape Agilent 4150 TapeStation System Manual...

  • Page 24: Screentape Architecture

    The gel contained within the ScreenTape device has been developed specifically to resolve nucleic acids. ScreenTape device The information is unique to each ScreenTape device. This includes: product details: ScreenTape assay type, product expiry date and a unique serial number. Agilent 4150 TapeStation System Manual...

  • Page 25: Product Descriptions

    Product Description Product Descriptions Product Descriptions 1 Overview of the 4150 TapeStation instrument Figure 4 Overview of the instrument Power button Lid release button Status LED Serial number Agilent 4150 TapeStation System Manual...
  • Page 26 ➔ Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields. 2 Magnet Figure 5 Position of magnet in the instrument lid Agilent 4150 TapeStation System Manual...
  • Page 27 The lid should be closed each time the TapeStation instrument is initialized or when the TapeStation Controller software is started. Close the lid by moving it gently downwards until latching is complete. Figure 6 Lid operation Agilent 4150 TapeStation System Manual...
  • Page 28 Product Description Product Descriptions 3 Overview of the instrument workspace Figure 7 Overview of the instrument workspace Gantry cover Upper nest cover Lower nest cover ScreenTape nest Tip waste Tip rack Sample tube strip holder Agilent 4150 TapeStation System Manual...
  • Page 29 Product Description Product Descriptions 4 Electrical connections Figure 8 1. Power line in, 2. USB-B to PC Remaining connectors are blocked by plugs and reserved for factory use. Agilent 4150 TapeStation System Manual...
  • Page 30 Figure 9 Status indicators Idle green Run mode yellow Not-ready, waiting for a specific pre-run condition to be reached or completed Error mode (restart required) red blinking Error mode (restart required) or ongoing firmware update Agilent 4150 TapeStation System Manual...
  • Page 31 Lid release button status light Blinking: Note the input request from the TapeStation Controller software Normal status Normal status is off. Blinking indicates an input request from the TapeStation Controller software to confirm a run stop request. Agilent 4150 TapeStation System Manual...

  • Page 32: Physical Specification

    The assay specific temperature range is measured at the ScreenTape nest. Choose the N O T E location of the instrument and the ambient conditions to fit to the working range of the assay else the start of runs will be disallowed. Agilent 4150 TapeStation System Manual...

  • Page 33: Installation

    Agilent 4150 TapeStation System Manual Installation Site Requirements Bench Space Required Unpacking Initial Setup of the System Prepare the Working Area This chapter gives information about the installation of the system. Agilent Technologies...

  • Page 34: Site Requirements

    Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. ➔...
  • Page 35 WA R N I N G Personal injury (for example electrical shock, intoxication) ➔ Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel. Agilent 4150 TapeStation System Manual...

  • Page 36: Bench Space Required

    Also pay special attention to the total weight of the modules you have ordered to ensure your laboratory bench can support this weight. The work bench should be stable and vibration free. Agilent 4150 TapeStation System Manual...
  • Page 37 Additional space of approximately 25 cm is required on top of the listed height in order to N O T E open the TapeStation lid. Please avoid locations with low overhanging shelves. Agilent 4150 TapeStation System Manual...

  • Page 38: Unpacking

    If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation. 1 Place the instrument box on the floor and cut it open. Agilent 4150 TapeStation System Manual...
  • Page 39 Installation Unpacking 2 Pull the instrument out of the outer cardboard box. 3 Turn the instrument over to a side. Agilent 4150 TapeStation System Manual...
  • Page 40 Installation Unpacking 4 Remove the outer cardboards and one part of the foam. Agilent 4150 TapeStation System Manual...
  • Page 41 Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields. 5 Lift the instrument out of the remaining foam onto a workbench. See step on page 26 for the position of the magnet in the instrument lid. Agilent 4150 TapeStation System Manual...
  • Page 42 Installation Unpacking 6 Open the lid. 7 Remove the workspace foam. 8 Remove the Vision lock tape. Agilent 4150 TapeStation System Manual...
  • Page 43 9 Carefully pull the gantry towards the front of the instrument. Damaged cables C AU T I O N Instrument damage at pump cables. ➔ Handle the foam carefully without touching or damaging the cables. 10 Carefully lift the pump foam upwards. Agilent 4150 TapeStation System Manual...
  • Page 44 Installation Unpacking 11 Push the gantry carefully towards the back of the instrument. 12 Close the lid. 13 Keep all packaging material for later storage or shipment. Agilent 4150 TapeStation System Manual...

  • Page 45: Initial Setup Of The System

    ➔ Make sure the power connector of the instrument can be easily reached and unplugged. ➔ Provide sufficient space behind the power socket of the instrument to unplug the cable. Agilent 4150 TapeStation System Manual...
  • Page 46 Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Absence of ground connection WA R N I N G The absence of ground connection can lead to electric shock or short circuit.
  • Page 47 Initial Setup of the System 2 Connect laptop to power according to its accompanying documentation. Refer to “Preparing the Instrument” on page 65 for the required order when turning the N O T E instrument on. Agilent 4150 TapeStation System Manual...

  • Page 48: Prepare The Working Area

    Installation Prepare the Working Area Prepare the Working Area 1 Install the tip waste. 2 Install the sample tube strip holder. Agilent 4150 TapeStation System Manual...
  • Page 49 Installation Prepare the Working Area 3 Install the tip rack and remove the lid. Agilent 4150 TapeStation System Manual...
  • Page 50 4 Keep the working area clean and tidy. Keep in mind the robotics movements during an analysis. Let material snap in place where N O T E appropriate. Do not leave lids, pipettes or packaging material in the working area. Agilent 4150 TapeStation System Manual...

  • Page 51: Tapestation Software

    Agilent 4150 TapeStation System Manual TapeStation Software TapeStation Controller Software TapeStation Controller Software User Interface TapeStation Controller Software Settings System Diagnostics TapeStation Analysis Software Agilent Information Center This chapter gives an overview on the three packages within the TapeStation software.

  • Page 52: Tapestation Controller Software

    The connection of only one single TapeStation system at a time to a computer running the N O T E TapeStation Controller software is allowed. Multiple instruments or fast switching between them will cause communication errors. Agilent 4150 TapeStation System Manual...

  • Page 53: Tapestation Controller Software User Interface

    • Sample tube strip functions, accessible by clicking onto '...', will allow you to select or clear all position and to decide if a run ladder or an electronic ladder is used. Table functions allow you to import or export sample descriptions or to clear the table. Agilent 4150 TapeStation System Manual...
  • Page 54 ScreenTape device is fully used or expired. In the status message area, upcoming maintenance and errors are also displayed. Agilent 4150 TapeStation System Manual...

  • Page 55: Tapestation Controller Software Settings

    “Exchange the Electrode Cartridge” page 135. • Run in instrument Demo Mode with virtual ScreenTape assays. • Start Hardware Diagnostics and Functional Verification context. • Start the Agilent Information Center. • See information about the software. Agilent 4150 TapeStation System Manual...
  • Page 56 You can create daily subdirectories within the location chosen as output path. The File Save To Selection allows you to change to a different location on your hard disk drive. Agilent 4150 TapeStation System Manual...

  • Page 57: System Diagnostics

    122). By default result files from these test suites are collected into exported log files for remote support purposes (see “Collecting Troubleshooting Information” page 221). Figure 14 TapeStation Controller software: System Diagnostics context Agilent 4150 TapeStation System Manual...

  • Page 58: Tapestation Analysis Software

    Within the Agilent Information Center (see “Agilent Information Center” page 60) you will find a detailed description for the TapeStation Analysis software user interface. Under Software Operations you can find How To videos for most common tasks. Agilent 4150 TapeStation System Manual...
  • Page 59 TapeStation Software TapeStation Analysis Software Figure 15 TapeStation Analysis software graphical interface Agilent 4150 TapeStation System Manual...

  • Page 60: Agilent Information Center

    Center also includes a copy of the context-sensitive help on the TapeStation software. The Agilent Information Center should be tailored to your needs with two simple steps: N O T E Select your language and select your instrument type. Agilent 4150 TapeStation System Manual...
  • Page 61 TapeStation Software Agilent Information Center Figure 16 Agilent Information Center for the TapeStation system Agilent 4150 TapeStation System Manual...
  • Page 62 TapeStation Software Agilent Information Center Agilent 4150 TapeStation System Manual...

  • Page 63: Operating Instructions

    Agilent 4150 TapeStation System Manual Operating Instructions Sample Preparation Preparing the Instrument Starting the Analysis Analysis End Follow Up This chapter explains the operation of the 4150 TapeStation system. Agilent Technologies...

  • Page 64: Sample Preparation

    178 and “Troubleshooting RNA Applications” on page 200. The assay-specific Quick Guides contain important information on the ladder, see also N O T E “Ladder Considerations” on page 92. Please read this section carefully. Agilent 4150 TapeStation System Manual...

  • Page 65: Preparing The Instrument

    Operating Instructions Preparing the Instrument Preparing the Instrument 1 Power on the laptop, wait until it is booted. 2 Turn On Figure 17 Power switch. 1: On; 2: Off Agilent 4150 TapeStation System Manual...
  • Page 66 3 Allow the instrument to initialize. 4 Start the TapeStation Controller software. In case the software doesn't start properly, see “Instrument and Laptop” on page 212. 5 Open the lid by pressing the lid release button. Agilent 4150 TapeStation System Manual...
  • Page 67 Operating Instructions Preparing the Instrument 6 Empty the tip waste. 7 Install the tip rack and remove the lid. Ensure sufficient tips are present. Agilent 4150 TapeStation System Manual...
  • Page 68 These bubbles often occur at the gel/buffer interface and need to be displaced prior to running. Failure to remove bubbles from the gel/buffer interface is detrimental to the performance of the ScreenTape assay. Agilent 4150 TapeStation System Manual...
  • Page 69 10 The TapeStation Controller software interface will change and displays the ScreenTape assay recognized. For details, see “TapeStation Controller Software” on page 52. 11 Centrifuge samples to remove spills and air bubbles. For details, see “Centrifugation Recommendations” on page 97. Agilent 4150 TapeStation System Manual...
  • Page 70 Blockage of the robotic system C AU T I O N ➔ Remove the lid from the sample tube strips to prevent blockage of the robotics system during pipetting steps. 12 Insert sample tube strips. Remove the caps. Agilent 4150 TapeStation System Manual...
  • Page 71 Quick Guides or the section “Ladder Considerations” page 92. Typically, one lane of ladder is run for every ScreenTape device used. Higher volumes of N O T E ladder are required for runs with multiple ScreenTape devices. Agilent 4150 TapeStation System Manual...
  • Page 72 Keep in mind the robotics movements during an analysis. Let material snap in place where N O T E appropriate. Do not leave lids, pipettes or packaging material in the working area. 15 Close the lid. Agilent 4150 TapeStation System Manual...

  • Page 73: Starting The Analysis

    1 Select samples for the analysis on the tube strip holder. 2 Enter sample descriptions. 3 Optional: Modify user name, notes, lot information and filename prefix. Add reagent lot and ScreenTape lot information. 4 Note required consumables: Make sure sufficient tips are present. Agilent 4150 TapeStation System Manual...
  • Page 74 In case of stopped runs, refer to “Assembling Nest Covers” on page 156, “Insertion of the Lower Nest Cover” on page 158 “Software Messages” on page 167 for further information. Agilent 4150 TapeStation System Manual...
  • Page 75 10 Instrument initialization and sample analysis take place. The remaining run time is displayed. 11 Sample analysis may be aborted immediately or stopped after sample analysis if desired. The lid button does blink while this dialog is displayed. Agilent 4150 TapeStation System Manual...
  • Page 76 Operating Instructions Starting the Analysis 12 Insufficient lanes on a ScreenTape devices were present. Add a second ScreenTape device with sufficient unused lanes within 15 min to finalize the analysis. Agilent 4150 TapeStation System Manual...

  • Page 77: Analysis End

    Software will launch in read-only mode after data from the first ScreenTape device is available. Data from the additional ScreenTape device are added to this read-only file until the second ScreenTape device has been run; then the file is finalized. Agilent 4150 TapeStation System Manual...

  • Page 78: Follow Up

    Operating Instructions Follow Up Follow Up After the analysis end follow the post run steps listed below. 1 Gather any partially used ScreenTape device from the nest and store it according to the Quick Guide instructions. Agilent 4150 TapeStation System Manual...
  • Page 79 Operating Instructions Follow Up 2 Empty the tip waste. 3 Remove the sample tube strip. Agilent 4150 TapeStation System Manual...
  • Page 80 Operating Instructions Follow Up 4 Cover the tip rack with lid until next use. 5 Close the lid. Agilent 4150 TapeStation System Manual...

  • Page 81: Good Measurement Practices

    Agilent 4150 TapeStation System Manual Good Measurement Practices Use of Correct Tools Tools and Handling Good Pipetting Practice Reagents and Reagent Mixes ScreenTape Devices Partially Used ScreenTape Devices Sample Preparation Ladder Considerations Use the Correct Protocol Mixing Recommendations Centrifugation Recommendations...
  • Page 82 This section describes techniques for ensuring reliable quantification and sizing results using all assays on the TapeStation systems. Latest Technical Notes as well as all other user information can be found on the Agilent website: https://www.agilent.com/en/product/tapestation-automated-electrophoresis. Agilent 4150 TapeStation System Manual...

  • Page 83: Use Of Correct Tools

    • After vortexing, use an appropriate centrifuge for either 96-well sample plates, or tube strips to ensure that all of the samples are at the bottom of the tube before placing in the TapeStation instrument. Agilent 4150 TapeStation System Manual...

  • Page 84: Tools And Handling

    • Always wear gloves to prevent contamination. • When pipetting samples, use loading tips that are of adequate size. Loading tips that are too large lead to poor quantitation accuracy. • Change loading tips between steps to avoid cross contamination. Agilent 4150 TapeStation System Manual...

  • Page 85: Good Pipetting Practice

    • Use a pipetting technique suitable for heterogenous samples. • Care must be taken due to viscosity of sample buffer. Highly viscous solutions may require drawing liquid more slowly to ensure that the proper volume is filled. Agilent 4150 TapeStation System Manual...

  • Page 86: Reagents And Reagent Mixes

    85, and the appropriate Quick Guide (Table 3 on page 64). • When pipetting small volumes ensure that no sample remains within the tip. Special care must be taken with high sensitivity assays which use small volumes of sample buffer. Agilent 4150 TapeStation System Manual...

  • Page 87: Screentape Devices

    If there are any small bubbles present at the gel/buffer interface, flicking the ScreenTape device will move them to the top of the chamber. Failure to remove air bubbles at the gel/buffer interface might significantly affect results. N O T E Agilent 4150 TapeStation System Manual...
  • Page 88 Assay performance specifications do not apply beyond the expiration date. Changing TapeStation Controller software settings will permit the instrument running expired ScreenTape devices, see “TapeStation Controller Software Settings” on page 55. Resulting data are marked accordingly. Agilent 4150 TapeStation System Manual...
  • Page 89 Bubbles within the gel lane significantly affect results. N O T E Figure 19 Different effects of bubbles in the gel matrix on TapeStation Analysis software results - Gel view Agilent 4150 TapeStation System Manual...

  • Page 90: Partially Used Screentape Devices

    Partially used ScreenTape devices have a 2 weeks use period after first use. Discard N O T E partially used ScreenTape devices beyond this time. A related message will show up in the TapeStation Controller software interface. Agilent 4150 TapeStation System Manual...

  • Page 91: Sample Preparation

    N O T E foil seal (96 -well Plate Foil Seal, Pack of 100 foils (5067-5154)) to prevent evaporation. The TapeStation system is capable of piercing through the foil, providing only Agilent approved foils are used. Agilent 4150 TapeStation System Manual...

  • Page 92: Ladder Considerations

    “TapeStation Controller Software User Interface” on page 53. Typically, one lane of ladder is run for every ScreenTape device used. Higher volumes of N O T E ladder are required for runs with multiple ScreenTape devices. Agilent 4150 TapeStation System Manual...

  • Page 93: Use The Correct Protocol

    • For correct volumes and quantitative range, please refer to the appropriate Quick Guide (“Sample Preparation” on page 64). Figure 20 Effect of correct (A1) and incorrect consumables/protocol (B1 – D1) on the D1000 Ladder peak intensity and Marker assignment in the D1000 ScreenTape assay. Agilent 4150 TapeStation System Manual...

  • Page 94: Mixing Recommendations

    Following the recommended procedure is the best way to attain accurate quantification results. The effects of insufficient mixing were investigated using the D1000 ScreenTape assay, and results are presented in the figures and table below. Agilent 4150 TapeStation System Manual...
  • Page 95 IKA vortexer and adaptor at 2000 rpm for 1 minute followed by brief centrifugation. Blue shows results for pipette mixing only. Red shows the effect of no mixing. Images were taken from the TapeStation Analysis software. Agilent 4150 TapeStation System Manual...
  • Page 96 TapeStation Analysis software and the Agilent D1000 ScreenTape assay when using the correct protocol (vortex mix followed by brief centrifugation) and two incorrect mixing protocols (pipette mixing only and no mixing) as illustrated in the figures above. Agilent 4150 TapeStation System Manual...

  • Page 97: Centrifugation Recommendations

    N O T E strips, both must be centrifuged. A centrifuge with a 8-tube strip rotor and a centrifuge with a rotor for 96-well sample plates can be used as well as devices with interchangeable rotors. Agilent 4150 TapeStation System Manual...
  • Page 98 B1) in comparison to a smeared Genomic DNA ladder and sample lane (C1 and D1) as well as a blank lane in the Gel View (Left) and Electropherogram View (Right) of the TapeStation Analysis software. Agilent 4150 TapeStation System Manual...

  • Page 99: Rna Assays

    “Troubleshooting RNA Applications” on page 200. Figure 25 Example of extra peaks being detected at the 28S position due to insufficient denaturation in the Gel View (Left) and Electropherogram View (Right) of the TapeStation Analysis software. Agilent 4150 TapeStation System Manual...

  • Page 100: General Recommendations When Working With Rna

    N O T E Figure 26 Example of a degraded RNA sample in the High Sensitivity RNA assay resulting in missing peak identification in the Gel View (Left) and Electropherogram View (Right) of the TapeStation Analysis software. Agilent 4150 TapeStation System Manual...

  • Page 101: Peak Integration

    In assays with no upper marker (RNA, High Sensitivity RNA, Genomic DNA, and Cell-free N O T E DNA assay) quantification is calculated using the lower marker. Figure 27 Correct upper marker integration for DNA ScreenTape assays. Figure shows D1000 ScreenTape assay. Agilent 4150 TapeStation System Manual...
  • Page 102 The displayed examples are chosen to show the effect of incorrect peak assignment to full N O T E extent. Samples with broad peaks as shown here should be analyzed in the Region view. See the Agilent Information Center for more information. Agilent 4150 TapeStation System Manual...
  • Page 103 Figure 29 Example of an incorrect (Left) assigned Upper Marker and how to identify the correct Upper Marker (Right) in the D1000 ScreenTape assay in the TapeStation Analysis software. Agilent 4150 TapeStation System Manual...

  • Page 104: Peak Maxima Versus Average Molecular Weight Sizing And Molarity

    The same holds true for quantification results, especially molarity as this is determined from both size and quantity. Errors in sizing and quantification result in erroneous molarity calculations. N O T E Agilent 4150 TapeStation System Manual...
  • Page 105 Peak Maxima Versus Average Molecular Weight Sizing and Molarity Figure 30 The sizing data obtained in Electropherogram and Region views of the TapeStation Analysis software. Figure 31 The concentration data obtained in Electropherogram and Region views of the TapeStation Analysis software. Agilent 4150 TapeStation System Manual...

  • Page 106: Sizing Range

    If applicable use an assay with a broader sizing range. Figure 33 Example of a sample within the sizing range of the High Sensitivity D5000 assay (left) and the same sample merging with the upper marker (right) in the High Sensitivity D1000 assay. Agilent 4150 TapeStation System Manual...

  • Page 107: Quantitative Range

    64). Figure 34 Example of missing marker peak due to too concentrated sample in the High Sensitivity D1000 assay in the Gel view (Left) and Electropherogram view (Right) of the TapeStation Analysis software. Agilent 4150 TapeStation System Manual...
  • Page 108 Quantitative range Figure 35 Example of missing sample peak due to too low concentrated sample in the High Sensitivity D1000 assay in the Gel view (Left) and Electropherogram view (Right) of the TapeStation Analysis software. Agilent 4150 TapeStation System Manual...
  • Page 109 Example of a sample within (A1) and outside (B1) the quantitative range of the High Sensitivity RNA assay in the Gel view (Left) and Comparison view (Right) of the TapeStation Analysis software. The Lower Marker peak (green arrow) is missing in the overconcentrated sample. Agilent 4150 TapeStation System Manual...

  • Page 110: Maximum Buffer Concentration In Sample

    (that is, the sample matrix) must not exceed these maximum salt concentrations. Otherwise the performance is significantly affected. The Maximum buffer concentration in sample can be found in the respective Quick Guide (Table 3 on page 64). Agilent 4150 TapeStation System Manual...
  • Page 111 Maximum Buffer Concentration in Sample Figure 37 Effects of increasing salt concentrations in the sample matrix on the RNA and High Sensitivity RNA Screentape Assay in the unaligned Gel view of the TapeStation Analysis software. Agilent 4150 TapeStation System Manual...

  • Page 112: Rine Functional Range

    64). Figure 38 Effects of overconcentrated samples on run distance of the Lower Marker and RIN values in the High Sensitivity RNA ScreenTape assay in the Gel view of the TapeStation Analysis software. Agilent 4150 TapeStation System Manual...

  • Page 113: Instrument Diagnosis

    Agilent 4150 TapeStation System Manual Instrument Diagnosis Instrument Diagnosis Tests Test Suite Groups Software Installation Verification System Verification Hardware Troubleshooting TapeStation Test Tape Executing System Verification Tests Reporting Reviewing Test Results This chapter contains information on instrument diagnosis, available tests, and reporting.

  • Page 114: Instrument Diagnosis

    System Diagnostics is designed for users and trained Agilent service personnel. System Diagnostics speeds up remote troubleshooting by providing detailed support information for any instrument error that may arise. Figure 39 TapeStation Controller software: System Diagnostics context Agilent 4150 TapeStation System Manual...

  • Page 115: Tests

    Resets the instrument to normal operational mode and requests removal of material. In case no valid TapeStation system is connected, only those tests belonging to the N O T E Software Installation Verification can be run. Agilent 4150 TapeStation System Manual...

  • Page 116: Software Installation Verification

    • TapeStation Test Tape (5067-5601) • Full rack of Loading Tips (5067-5598) • Pipet tip waste bin It is required to ensure the ScreenTape nest covers and the gantry cover are in place during execution of the System Verification. Agilent 4150 TapeStation System Manual...

  • Page 117: Hardware Troubleshooting

    Please collect liquid at the bottom of the vial by centrifugation before starting the liquid handling check. Spilled liquid or trapped air bubbles will affect the results. Please refer to “Centrifugation Recommendations” on page 97. Agilent 4150 TapeStation System Manual...

  • Page 118: Tapestation Test Tape

    117) as directed by your support representative. Dialogues during test suite execution (see “Executing System Verification Tests” on page 120) will request you to insert this TapeStation Test Tape to the ScreenTape nest. Agilent 4150 TapeStation System Manual...
  • Page 119 Upon insertion of the TapeStation Test Tape to a TapeStation system that is connected to the TapeStation Controller software, the start of the System Diagnostics context is triggered. A dialog will guide you through this process. Agilent 4150 TapeStation System Manual...

  • Page 120: Executing System Verification Tests

    Some individual tests are depended on others. When a test is deselected, any dependent N O T E tests are also deselected. 7 Click Start and follow the on-screen instructions closely. Agilent 4150 TapeStation System Manual...
  • Page 121 “Agilent Information Center” on page 60) and search for details on root causes in the section on System Diagnostics. Consult the section “Collecting Troubleshooting Information” on page 221 before contacting the Agilent technical support experts. Agilent 4150 TapeStation System Manual...

  • Page 122: Reporting

    From the TapeStation Analysis software log files in ZIP format can be exported to be sent N O T E to Agilent technical support experts. See “Collecting Troubleshooting Information” page 221 for details. These log files will automatically contain the latest test results. Agilent 4150 TapeStation System Manual...

  • Page 123: Maintenance

    Electrode Cartridge Exchange Process Detail Exchange the Electrode Cartridge Cleaning the Instrument Preparing the Instrument for Transportation Boxing for Transportation Assembling Nest Covers Insertion of the Lower Nest Cover This chapter describes the maintenance of the 4150 TapeStation system. Agilent Technologies...

  • Page 124: Maintenance Information

    An unused Needle Change Cartridge (5067-5783) is prerequisite for the needle exchange and is recognized by the software. The Needle Change Cartridge is designed for one time use. An extracted piercing needle cannot be remounted. Agilent 4150 TapeStation System Manual...

  • Page 125: Maintenance Counter

    50000 samples is reached or the Electrode Cartridge change is due. Only in the 4150 TapeStation system the Electrode Cartridge can be changed either by a N O T E trained service engineer during a preventive maintenance or by the user. Scheduled a preventive maintenance service by a trained service engineer for the 4200 TapeStation system.

  • Page 126: Preventive Maintenance

    • firmware and software update if applicable, • general cleaning of instrument, • execution of functional hardware test. For the 4150 TapeStation system most preventive maintenance tasks can be done by the user using information provided in the user guide. For instructions, see “Exchange the Electrode Cartridge”...

  • Page 127: Exchange The Piercing Needle

    1 Prepare instrument by selecting Tools > Maintenance > Change needle from the TapeStation Controller software. The exchange process opens the ScreenTape nest to allow insertion of the Needle Change Cartridge. 2 Close Lid. Click Proceed. Agilent 4150 TapeStation System Manual...
  • Page 128 4 Click Proceed to continue. 5 After exchange: Remove Needle Change Cartridge from ScreenTape nest and discard. The used cartridge has an open slot on the left side and the old piercing needle in the slot on the right. Agilent 4150 TapeStation System Manual...
  • Page 129 7 Wait approximately two minutes until the automated pierce needle calibration is complete. It is crucial not to turn off the instrument or terminate the software during the needle exchange; this could impact performance of all future analyses. Agilent 4150 TapeStation System Manual...

  • Page 130: Electrode Cartridge

    In the 4200 TapeStation system, the Electrode Cartridge must be exchanged only by a trained service engineer during a scheduled preventive maintenance service. In the 4150 TapeStation system, the Electrode Cartridge can be changed either by a trained service engineer during a preventive maintenance or by the user.
  • Page 131 Cartridge exchange procedure. Remove the Electrode Cartridge only when prompted by a software dialog. Only in the 4150 TapeStation system the Electrode Cartridge can be changed either by a N O T E trained service engineer during a preventive maintenance or by the user. Schedule a preventive maintenance service in case of the 4200 TapeStation system.

  • Page 132: Additional Functionality Of The Electrode Cartridge

    • Electrode cartridge Serial number • Type of construction • Date of first use • Sample counter This information is accessed and displayed when diagnostic counters or results of the Electrode Cartridge History test are displayed. Agilent 4150 TapeStation System Manual...

  • Page 133: Electrode Cartridge Exchange Process Detail

    • The inserted Electrode Cartridge was successfully registered • The date of first use is the current day • No samples were analyzed with it • The electrical functionality test with the TapeStation Test Tape passed Agilent 4150 TapeStation System Manual...
  • Page 134 The instrument will remain inoperable until a correct and valid cartridge is installed. Please collect and provide troubleshooting information (“Collecting Troubleshooting Information” on page 221) and schedule a service visit if you cannot correct the error yourself. Agilent 4150 TapeStation System Manual...

  • Page 135: Exchange The Electrode Cartridge

    1 During a preventive maintenance service the Electrode Cartridge is exchanged. For details, see “Electrode Cartridge” on page 130. 2 Prepare the instrument by navigating to Settings‚ Maintenance, and Change Electrode Cartridge. Follow further onscreen instructions. 3 Open the lid. Agilent 4150 TapeStation System Manual...
  • Page 136 Electrode Cartridge 4 Insert a TapeStation Test Tape. 5 Close the lid. 6 Click Proceed. 7 The Electrode Cartridge is deactivated. The instrument remains inoperable until the following steps are performed successfully. Follow further onscreen instructions. Agilent 4150 TapeStation System Manual...
  • Page 137 Maintenance Electrode Cartridge 8 Open the lid. 9 Remove the TapeStation Test Tape. 10 Remove the upper nest cover. Agilent 4150 TapeStation System Manual...
  • Page 138 Instrument malfunction and loss of material. ➔ Do not touch the lower nest cover. 11 Do not alter the installed lower nest cover. Keep the lower nest cover in place. 12 Click Proceed. Follow further onscreen instructions. Agilent 4150 TapeStation System Manual...
  • Page 139 Electrode Cartridge 13 Release the cartridge lever with a push and bring it to the upright position. 14 Pull the Electrode Cartridge out in a straight upward movement. 15 Click Proceed. Follow further onscreen instructions. Agilent 4150 TapeStation System Manual...
  • Page 140 Collision of the electrode pins with any part of the ScreenTape nest can damage the Electrode Cartridge. ➔ Replace the Electrode Cartridge carefully. 16 Insert the new Electrode Cartridge carefully. Push downwards until it reaches a resistance. Agilent 4150 TapeStation System Manual...
  • Page 141 Maintenance Electrode Cartridge 17 Move the lever to the lock position. An internal locking bolt positions the Electrode Cartridge correctly. 18 Click Proceed. Follow further onscreen instructions. Agilent 4150 TapeStation System Manual...
  • Page 142 Maintenance Electrode Cartridge 19 Reinsert the upper nest cover: Guide the rear positioning extensions into the corresponding recesses under the gantry. Lower the cover until the magnets hold it in place. Agilent 4150 TapeStation System Manual...
  • Page 143 Maintenance Electrode Cartridge 20 Click Proceed. Follow further onscreen instructions. Agilent 4150 TapeStation System Manual...
  • Page 144 22 Close the lid. 23 Click Proceed to start a short verification. 24 Click Proceed to start System Verification tests. If the Electrode Cartridge Change was not successful, please refer to “Troubleshooting” on page 165. Agilent 4150 TapeStation System Manual...
  • Page 145 Please be aware of software notifications that are related to the Electrode Cartridge: • “Electrode Cartridge Change Required” on page 174 • “Used Electrode Cartridge Inserted” on page 175 • “Electrode Cartridge Change has Failed” on page 176 • “Electrode Cartridge not Detected” on page 177 Agilent 4150 TapeStation System Manual...

  • Page 146: Cleaning The Instrument

    Heat deformation of plastic components C AU T I O N Deformed plastic components will create blockage errors during instrument operation. ➔ Do not expose plastic components to heat. ➔ Do not use a dishwasher. Agilent 4150 TapeStation System Manual...

  • Page 147: Preparing The Instrument For Transportation

    A piercing needle is located behind the gantry cover. WA R N I N G ➔ Do not reach under the cover. ➔ Do not remove the cover. 1 Provide dedicated packaging material for later use. Agilent 4150 TapeStation System Manual...
  • Page 148 4 Select Prepare Transportation from the TapeStation Controller software menu. The software guides you through steps listed below for fixation all robotic parts to a safe position. 5 Open the lid by pressing the lid release button. Agilent 4150 TapeStation System Manual...
  • Page 149 Preparing the Instrument for Transportation 6 Carefully pull the gantry towards the front of the instrument. 7 Move the pump to the middle of the axis. In case of defective instruments, this has to be done manually. Agilent 4150 TapeStation System Manual...
  • Page 150 Instrument damage at pump cables. ➔ Handle the foam carefully without touching or damaging the cables. 8 Install the pump foam carefully over the pump. 9 Push the gantry carefully towards the back of the instrument. Agilent 4150 TapeStation System Manual...
  • Page 151 Insertion of the Vision lock tape is only possible if the camera is in its default position on N O T E the right side. 12 Click Proceed Agilent 4150 TapeStation System Manual...
  • Page 152 Maintenance Preparing the Instrument for Transportation 13 Insert the workspace foam. 14 Close the lid. 15 Close the TapeStation Controller software. 16 Power down the instrument. Agilent 4150 TapeStation System Manual...

  • Page 153: Boxing For Transportation

    Protect the instrument by transport protection material, see step 13 on page 44 ➔ Store the transport protection material in a safe place to use for later transport of the instrument. 1 Lift the instrument into one foam part. Agilent 4150 TapeStation System Manual...
  • Page 154 Maintenance Boxing for Transportation 2 Mount the second foam part. 3 Mount both cardboards. 4 Turn the instrument over to a side. Agilent 4150 TapeStation System Manual...
  • Page 155 Maintenance Boxing for Transportation 5 Slide the instrument into the box. Agilent 4150 TapeStation System Manual...

  • Page 156: Assembling Nest Covers

    Maintenance Assembling Nest Covers Assembling Nest Covers 1 Reinsert the upper nest cover: Guide the rear positioning extensions into the corresponding recesses under the gantry. Lower the cover until the magnets hold it in place. Agilent 4150 TapeStation System Manual...
  • Page 157 Maintenance Assembling Nest Covers Agilent 4150 TapeStation System Manual...

  • Page 158: Insertion Of The Lower Nest Cover

    It is unnecessary to remove the lower nest cover. Follow the instructions in case this was done by accident. Lower nest cover incorrectly mounted C AU T I O N Instrument malfunction and loss of material. ➔ Do not touch the lower nest cover. Agilent 4150 TapeStation System Manual...
  • Page 159 Maintenance Insertion of the Lower Nest Cover 1 Carefully touch the LED module at its top and move the assembly to the left side. Agilent 4150 TapeStation System Manual...
  • Page 160 Maintenance Insertion of the Lower Nest Cover 2 Mount the lower nest cover to the lever such that the half-round cutout meets its counter part on the lever. Agilent 4150 TapeStation System Manual...
  • Page 161 Maintenance Insertion of the Lower Nest Cover 3 Reinsert the upper nest cover: Guide the rear positioning extensions into the corresponding recesses under the gantry. Lower the cover until the magnets hold it in place. Agilent 4150 TapeStation System Manual...
  • Page 162 Maintenance Insertion of the Lower Nest Cover Agilent 4150 TapeStation System Manual...
  • Page 163 3 cm. 5 Move the gantry gently back again. The lower nest cover moves forward. 6 The lower nest cover mounting is correct if it moves smoothly in opposite direction to the gantry cover. Agilent 4150 TapeStation System Manual...
  • Page 164 Maintenance Insertion of the Lower Nest Cover Agilent 4150 TapeStation System Manual...

  • Page 165: Troubleshooting

    Agilent 4150 TapeStation System Manual Troubleshooting Software Messages TapeStation Test Tape Detected Camera Fault Detected Run Stopped During Initialization Process Block at Lower Nest Cover Insufficient Lanes Detected Premature End of Analysis Perform Needle Change Electrode Cartridge Change Required Used Electrode Cartridge Inserted...
  • Page 166 Error Scenario: Unexpected Termination of the TapeStation Controller Software Error scenario: ScreenTape barcode not detected Collecting Troubleshooting Information This chapter gives an overview about the troubleshooting features of the 4150 TapeStation system. It includes information about application troubleshooting as well. Agilent 4150 TapeStation System Manual...

  • Page 167: Software Messages

    Troubleshooting Software Messages Software Messages TapeStation Test Tape Detected 1 A TapeStation Test Tape (see “TapeStation Test Tape” on page 118) detected in the ScreenTape nest allows you to start the System Diagnostics. Agilent 4150 TapeStation System Manual...

  • Page 168: Camera Fault Detected

    1 The TapeStation Controller software could not connect to the instruments' camera. Check USB connection to the instrument, power-cycle the instrument and restart the software. In case of recurrence reboot the laptop. See “Instrument Communication” on page 212 for details. Agilent 4150 TapeStation System Manual...

  • Page 169: Run Stopped During Initialization Process

    Run Stopped During Initialization Process 1 The consumables required for a run were not detected. Provide sufficient loading tips in the tip rack. Remove tube strip caps, tip rack cover and empty the waste locations. Agilent 4150 TapeStation System Manual...

  • Page 170: Block At Lower Nest Cover

    Troubleshooting Software Messages Block at Lower Nest Cover 1 The lower nest cover did not move and requires proper assembly. See “Insertion of the Lower Nest Cover” on page 158 for instructions. Agilent 4150 TapeStation System Manual...

  • Page 171: Insufficient Lanes Detected

    Troubleshooting Software Messages Insufficient Lanes Detected 1 Insufficient lanes on a ScreenTape devices were present. Add a second ScreenTape device with sufficient unused lanes within 15 min to finalize the analysis. Agilent 4150 TapeStation System Manual...

  • Page 172: Premature End Of Analysis

    1 Insufficient number of correct ScreenTape devices in the rack leads to a premature end of the analysis. Consider that prepared samples which did not run due to an error might be aged and might not be analyzable. Agilent 4150 TapeStation System Manual...

  • Page 173: Perform Needle Change

    1 No further analysis is possible beyond 4480 samples until a needle exchange is done. A warning message which recommends this appears already after 3840 samples. See “Exchange the Piercing Needle” page 127 for further information. Agilent 4150 TapeStation System Manual...

  • Page 174: Electrode Cartridge Change Required

    Troubleshooting Software Messages Electrode Cartridge Change Required 1 An Electrode Cardridge Change is required after either 50000 samples or after a year of usage, whatever comes first. See further information “Electrode Cartridge” on page 130. Agilent 4150 TapeStation System Manual...

  • Page 175: Used Electrode Cartridge Inserted

    1 The Electrode Cartridge inserted was previously used. Check the values for first use date and number of samples analyzed. In case this is intentional, continue to verify functionality with the System Diagnostic tests. For further information see “Electrode Cartridge” on page 130. Agilent 4150 TapeStation System Manual...

  • Page 176: Electrode Cartridge Change Has Failed

    1 An electrical functionality test with the TapeStation Test Tape failed on one or more lanes. Verify this result with the System Diagnostic tests and install a functional electrode cartridge. See further information “Electrode Cartridge” on page 130. Agilent 4150 TapeStation System Manual...

  • Page 177: Electrode Cartridge Not Detected

    1 The instrument is inoperable because the Electrode Cartridge is removed, incorrectly installed or defective. Click Ok to start the installation process with a correct and valid cartridge. See further information “Electrode Cartridge” on page 130. Agilent 4150 TapeStation System Manual...

  • Page 178: Troubleshooting Dna Applications

    Peaks that are designated as well are too large to have migrated into the gel; please be N O T E aware that an accurate concentration for the entire sample cannot be generated. Figure 44 Visualizing a well peak in the Electropherogram view of the TapeStation Analysis software. Agilent 4150 TapeStation System Manual...
  • Page 179 Ladder has been handled as directed prior to analysis on the Genomic DNA ScreenTape assay. B) The Genomic DNA Ladder vial was handled incorrectly - degradation of the top fragment (48500 bp) is clearly shown. Agilent 4150 TapeStation System Manual...

  • Page 180: Incorrect Quantification Results

    See “Peak analyzing samples with broad peaks. Region Maxima Versus Average Molecular Weight view uses a different method to calculate Sizing and Molarity” on page 104 for more concentration and sizing. information. Agilent 4150 TapeStation System Manual...
  • Page 181 Always cover with the recommended foil seal time if a 96-well sample plate was used which (PN 5067-5154) and ensure tight sealing was not sealed with the recommended seal foil. throughout the 96-well sample plate. Agilent 4150 TapeStation System Manual...
  • Page 182 See the Agilent Information Center for more information. Ensure that all sample peaks are within the recommended sizing range for the application. Agilent 4150 TapeStation System Manual...

  • Page 183: Incorrect Sizing Results

    For best sizing precision and accuracy, the user should run the appropriate ladder with the N O T E samples. Agilent 4150 TapeStation System Manual...
  • Page 184 “Maximum Buffer Aligned button in the TapeStation Analysis Concentration in Sample” on page 110. software. Please refer to the salt tolerance guidelines for your assay in the appropriate Quick Guide (Table 3 on page 64). Agilent 4150 TapeStation System Manual...

  • Page 185: Missing Marker Peaks

    Aligned button. See distance. “Unexpected Migration Profile” on page 188, “Maximum Buffer Concentration in Sample” page 110, and “Quantitative range” page 107. Blank lane See the Agilent Information Center for more information. Agilent 4150 TapeStation System Manual...

  • Page 186: Incorrect Or Missing Din Value

    Compare to aligned/unaligned data of good and questionable samples; then assign correct markers by right-clicking. See the Agilent Information Center for more information. Ensure that all sample peaks are within the recommended sizing range for the application. Agilent 4150 TapeStation System Manual...
  • Page 187 Check the electropherogram trace for peaks in lane containing more than 14 peaks as this incorrectly assigned peaks and judge if these potentially represents the ladder. are genuine peaks. Rerun samples with fresh reagents if in doubt. Agilent 4150 TapeStation System Manual...

  • Page 188: Unexpected Migration Profile

    See the Agilent Information Center for more information. Ensure that all sample peaks are within the recommended sizing range for the application, “Peak Integration” on page 101. Agilent 4150 TapeStation System Manual...
  • Page 189 Such bubbles can form the data. Those after the expiration date or upon wrong bubbles cannot be storage. See the Agilent Information Center for flicked away. more information. Agilent 4150 TapeStation System Manual...
  • Page 190 107. Use the High Sensitivity assay if applicable. Insufficient Mixing Buffer and sample “Mixing Recommendations” on page 94 was not sufficiently and the Agilent Information Center for more vortexed. The dye information. could not incorporate completely into the sample. Agilent 4150 TapeStation System Manual...
  • Page 191 See “Sample Preparation” on page 91 steps might lead to for more information Reagent Buffer or sample being contaminated with ladder. Agilent 4150 TapeStation System Manual...
  • Page 192 107. Use the High Sensitivity assay if applicable. Figure 46 Example of intense upper and lower markers seen in the blue gel image. Lower markers are normalized in electropherogram view and appear identical in signal. Agilent 4150 TapeStation System Manual...
  • Page 193 Table 3 storage accelerate aging of page 64). the ScreenTape device and reduce the shelf life. Storing reagents incorrectly may induce degradation of the dye. Both can lead to a higher than normal background noise. Agilent 4150 TapeStation System Manual...
  • Page 194 Quick Guide; and then prepare a new run, see in the specification “Quantitative range” on page 107. Use the table. High Sensitivity assay if applicable. Agilent 4150 TapeStation System Manual...
  • Page 195 See the Agilent caused by bubbles at interface may disrupt Information Center and “ScreenTape gel-buffer interface current flow between Devices” on page 87 for more information. the electrodes. Agilent 4150 TapeStation System Manual...

  • Page 196: Cell-Free Dna Assay

    Quick Guide (Table 3 on page 64). Figure 47 Effect of increasing EDTA concentration on lower marker run distance in aligned (left) and unaligned (right) gel view in the TapeStation Analysis software. Agilent 4150 TapeStation System Manual...
  • Page 197 %cfscore is not impaired in such a case. Figure 48 Genomic DNA contamination in a cell-free DNA sample as single peak (left) and split peak (right) in the electropherogram view in the TapeStation Analysis software. Agilent 4150 TapeStation System Manual...

  • Page 198: Tapestation Analysis In The Agilent Sureselect And Haloplex Workflow

    This artifact can be avoided by increasing the time for which the samples are incubated on the magnetic plate to 10 minutes, thereby removing a higher percentage of the beads. Figure 49 Electropherogram image with additional signal from AMPure beads above the Upper Marker peak (arrow) Agilent 4150 TapeStation System Manual...
  • Page 199 For examples with expected results within an Agilent SureSelect or HaloPlex workflow please see the demo files available in the TapeStation Analysis software. Figure 50 Electropherogram image with additional peak in a library smear (arrow) Agilent 4150 TapeStation System Manual...

  • Page 200: Troubleshooting Rna Applications

    “Good Pipetting Practice” on page 85 and technique or pipette pipettes can lead to poor pipetting performance follow guidelines of the respective pipette calibration which in turn alter sample/buffer ratio in an vendor. unpredictable manner. Agilent 4150 TapeStation System Manual...
  • Page 201 Contaminating beads can alter the run behavior If using the Sure Select Protocol, ensure that all of the sample and can introduce artifacts. AMPure beads are removed by increasing the time that the sample is on the magnetic plate. Agilent 4150 TapeStation System Manual...

  • Page 202: Missing Marker Peaks

    Aligned button. See distance. “Unexpected Migration Profile” on page 205, “Maximum Buffer Concentration in Sample” page 110, and “Quantitative range” page 107. Blank lane See the Agilent Information Center for more information. Agilent 4150 TapeStation System Manual...

  • Page 203: Degraded Rna Ladder And/Or Samples

    RNAse contamination will lead to degradation Ensure Good Measurement Practices when contamination of the of the sample. working with RNA assays; then prepare a new plasticware run using new reagents (“Good Measurement Practices” on page 81). Agilent 4150 TapeStation System Manual...

  • Page 204: Incorrect Or Missing Rine Value

    The 18S peak may have partially reverted back Samples should be kept on ice after to its original, non-denatured conformation. preparation, and run within 2 hours of denaturation, see “Heat Denaturation” page 99 for more information. Agilent 4150 TapeStation System Manual...

  • Page 205: Incorrect Or Missing Peak Annotation

    18S or 28S peak Unexpected Migration Profile There are a number of issues which can affect migration and sample peak profile. If the issues below do not describe your analysis file, please contact your support representative. Agilent 4150 TapeStation System Manual...
  • Page 206 Such bubbles can form after the the data. Those bubbles expiration date or upon wrong storage. See the cannot be flicked away Agilent Information Center or “ScreenTape Devices” on page 87 for more information Agilent 4150 TapeStation System Manual...
  • Page 207 Compare to aligned/unaligned data of good and as marker if running questionable samples; then assign correct very close to the actual markers by right-clicking. marker See the Agilent Information Center for more information Agilent 4150 TapeStation System Manual...
  • Page 208 Use the High Sensitivity assay if applicable Insufficient Mixing Buffer and sample were “Mixing Recommendations” on page 94 not sufficiently and the Agilent Information Center for more vortexed. The dye could information not incorporate completely into the sample Agilent 4150 TapeStation System Manual...
  • Page 209 “Sample Preparation” on page 91 for more lead to Reagent Buffer information or sample being contaminated with ladder. Agilent 4150 TapeStation System Manual...
  • Page 210 Table 3 on page 64) storage accelerate aging of the ScreenTape device and reduce the shelf life. Storing reagents incorrectly may induce degradation of the dye. Both can lead to a higher than normal background noise Agilent 4150 TapeStation System Manual...
  • Page 211 See the Agilent bubbles at gel-buffer disrupt current flow Information Center and “ScreenTape interface between the electrodes Devices” on page 87 for more information Agilent 4150 TapeStation System Manual...

  • Page 212: Instrument Communication

    • Always download antivirus software onto the TapeStation laptops. See the latest ReadMe.pdf for known problems or limitations. • Keep your system up to date with Windows updates. Agilent 4150 TapeStation System Manual...

  • Page 213: Usb Connection

    Other bundled laptops will vary in details. The three relevant items associated with the connection of the TapeStation instrument are: 1 USB Serial Port (COM x) 2 uEye UI-148xLE Series 3 USB Serial Converter Agilent 4150 TapeStation System Manual...
  • Page 214 Executing the System diagnosis ( “Instrument Diagnosis” on page 114) to verify the N O T E suitability of your laptop and proper functions of your instrument assists you to identify and correct connection issues of the system. Agilent 4150 TapeStation System Manual...

  • Page 215: Universal Corrective Actions

    1 Carefully backup important data first. 2 Reimaging is done with the media provided in the shipment of the TapeStation system. 3 Set up the Windows operating system with regional settings English-US. Agilent 4150 TapeStation System Manual...

  • Page 216: Error Scenario: Instrument Not Connecting

    1 Follow the instructions provided by the software. 2 Execute the System Diagnostics ( “Instrument Diagnosis” on page 114) to verify the suitability of your laptop and proper functions of your instrument. 3 Review failed tests and correct detected issues, if feasible. Agilent 4150 TapeStation System Manual...
  • Page 217 Reinstall the TapeStation software package. installed or malfunctioning. Make sure all required Windows updates are installed. See the latest ReadMe.pdf for reference. Refer to “Collecting Troubleshooting Information” on page 221 and contact your local N O T E support. Agilent 4150 TapeStation System Manual...

  • Page 218: Error Scenario: Unexpected Termination Of The Tapestation Controller Software

    In case a run with multiple tapes is terminated prematurely only that data is available that N O T E was recorded until the last completely processed ScreenTape device. Agilent 4150 TapeStation System Manual...
  • Page 219 Unforeseen activities or clicks in TapeStation Reduce activities such as changing preferred Controller software. settings within the TapeStation Controller software to a minimum. Refer to “Collecting Troubleshooting Information” on page 221 and contact your local N O T E support. Agilent 4150 TapeStation System Manual...

  • Page 220: Error Scenario: Screentape Barcode Not Detected

    Data transfer issue of camera due to general Follow the “Universal Corrective Actions” communication issues. page 215 for communication troubleshooting. Refer to “Collecting Troubleshooting Information” on page 221 and contact your local N O T E support. Agilent 4150 TapeStation System Manual...

  • Page 221: Collecting Troubleshooting Information

    Agilent technical support experts. Be aware that this package of log files does not include data files, experimental information or sample information. To properly describe your issue completely, you need to collect and compile this information in addition. Agilent 4150 TapeStation System Manual...
  • Page 222 Troubleshooting Collecting Troubleshooting Information Agilent 4150 TapeStation System Manual...
  • Page 223 Agilent 4150 TapeStation System Manual Appendix Accessories Shipment List This chapter contains parts information. Agilent Technologies...

  • Page 224: Appendix Accessories Shipment List

    Software and User Information, USB stick and Booklet, Box 5067-5601 TapeStation Test Tape 5188-8047 USB cable A to B, 2 m G2992-40042 TapeStation Tube Strip Holder, tray for 16 tubes G2992-40046 TapeStation Tip Waste Bucket, 1 bucket Agilent 4150 TapeStation System Manual...
  • Page 225 Loading Tips (5067-5599) in order to run more ScreenTape assays. Other orderable equipment: Description 5067-5783 Needle Change Cartridge 5067-5599 Loading Tips 10 packs of 112 tips 5067-5786 32-Pin Electrode Cartridge G2999AA TapeStation laptop with Pre-installed TapeStation software, bundle Agilent 4150 TapeStation System Manual...
  • Page 226 DIN value incorrect missing ladder considerations quantification laptop incorrect incorrect sizing results troubleshooting Agilent 4150 TapeStation System Manual...
  • Page 227 WEEE directive preparation workflow ScreenTape architecture HaloPlex ScreenTape devices SureSelect partially used ScreenTape settings controller software software installation verification software analysis controller software settings controller software user interface controller symbols system verification tests system verification Agilent 4150 TapeStation System Manual...
  • Page 228 • Introduction to the system • Site requirements and specifications • Installation • Software • Operation of the system • Instrument diagnosis • Maintenance • Troubleshooting • Product notices © Agilent Technologies 2018 Printed in Germany 12/2018 *G2992-90000* *G2992-90000* G2992-90000 Agilent Technologies...

Table of Contents