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Agilent Technologies 2200 TapeStation User Manual

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  • Page 1 Agilent 2200 TapeStation User Manual Agilent Technologies...
  • Page 2 Notices Warranty © Agilent Technologies, Inc. 2011, 2012 receive no greater than Restricted Rights as defined in FAR 52.227-19(c)(1-2) (June No part of this manual may be reproduced The material contained in this docu- 1987). U.S. Government users will receive in any form or by any means (including elec- ment is provided “as is,”...

  • Page 3: Table Of Contents

    Contents Contents 1 Introduction to the 2200 TapeStation System Overview of the System 2 Specifications Technical Specifications 3 Installing the System Unpacking the System Contents of the 2200 ScreenPlex TapeStation Installing the System 4 Using the 2200 TapeStation System Intended Use of the 2200 TapeStation System...

  • Page 4: Tapestation

    Contents 7 Appendix Product Notices Sound Emission The Waste Electrical and Electronic Equipment Directive Technical Service 2200 TapeStation...

  • Page 5: Introduction To The 2200 Tapestation System

    2200 TapeStation Introduction to the 2200 TapeStation System Overview of the System ScreenTape Architecture Agilent 2200 TapeStation Components This chapter gives an introduction to the system Agilent Technologies...

  • Page 6: Overview Of The System

    (ScreenTape), an instrument (the 2200 TapeStation) and an analysis software. The system is very straightforward to use, simply place the sample tubes and ScreenTape in to the 2200 TapeStation and let it load, separate, image, analyse and present the results.
  • Page 7 Introduction to the 2200 TapeStation System Overview of the System Information pertaining to the 2200 TapeStation can be found in: • 2200 TapeStation Technical Specification • 2200 TapeStation Components • Installing the 2200 TapeStation Information pertaining to sample and ScreenTape requirements can be found •...

  • Page 8: Agilent 2200 Tapestation Components

    USB Socket: The USB connector is inserted into the USB socket to link the laptop to the 2200 TapeStation. Power-cable socket: The power cable must be connected to the 2200 TapeStation and the relevant mains electricity outlet. 2200 TapeStation...
  • Page 9 Introduction to the 2200 TapeStation System Overview of the System ScreenTape Tip holder Sample block Figure 2 2200 TapeStation (front view) On/off switch Power-cable socket USB socket Figure 3 2200 TapeStation (back view) 2200 TapeStation...
  • Page 10 Introduction to the 2200 TapeStation System Overview of the System 2200 TapeStation...
  • Page 11 2200 TapeStation Specifications Technical Specifications This chapter provides information on the specifications of the 2200 TapeStation. Agilent Technologies...

  • Page 12: Specifications Technical Specifications

    Specifications Technical Specifications Technical Specifications 2200 TapeStation Input voltage: 12 V DC Power consumption: 40 W (VA) Current: Interface: USB cable (PC comms.) Instrument Housing: UL94/VO rated flame retardant cast polyurethane Dimensions: 400 x 310 x 310 mm Weight: 12.5 kg...
  • Page 13 Check that any air conditioning is functioning. • If the quoted current temperature is below the specified range please allow the instrument to equilibrate to the ambient temperature, and avoid using in a cooled area. 2200 TapeStation...
  • Page 14 Specifications Technical Specifications 2200 TapeStation...

  • Page 15: Installing The System

    2200 TapeStation Installing the System Unpacking the System Contents of the 2200 ScreenPlex TapeStation The TapeStation ScreenTape Products Installing the System Software Installation Agilent 2200 TapeStation Set Up This chapter gives information about how to install the system. Agilent Technologies...

  • Page 16: Unpacking The System

    Unpacking the System Unpacking the System Unpacking the Agilent 2200 TapeStation Do not attempt to unpack the 2200 TapeStation instrument until you have read the accom- panying Site and Safety Manual. Condensation within the instrument C A U T I O N Condensation will damage the system electronics.
  • Page 17 Ensure all parts and materials have been delivered with your system. The delivery checklist is shown below. Please report any missing or damaged parts to your local Agilent Technologies sales and service office. Please note that ScreenTape and Reagent kits are not provided with instrument delivery N O T E and must be purchased separately.

  • Page 18: Contents Of The 2200 Screenplex Tapestation

    Installing the System Contents of the 2200 ScreenPlex TapeStation Contents of the 2200 ScreenPlex TapeStation The TapeStation Table 1 Agilent 2200 TapeStation ScreenPlex System (G2966AA) Product Volume Properties Agilent 2200 The instrument for loading, electrophoresing, imaging and analysing double TapeStation...

  • Page 19: Screentape Products

    Installing the System Contents of the 2200 ScreenPlex TapeStation ScreenTape Products 5067-5373 ScreenTape DS12 7 ScreenTape 5067-5374 ScreenTape DS12 Reagents 1 vial • DS12 Ladder 150 µL • DS12 Loading Buffer 800 µL 2200 TapeStation...

  • Page 20: Installing The System

    Installing the System Installing the System Software Installation The software for your Agilent 2200 TapeStation system is preinstalled on the system laptop. For updates, or if you have to change the laptop, you may download the latest version of the N O T E software from the Test Kit Manufacturer's website.

  • Page 21: Agilent 2200 Tapestation Set Up

    If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. For general safety information, please refer to the 2200 TapeStation System - Site and N O T E Safety Manual.
  • Page 22 As there is more than one driver that can be detected and installed, you may need to follow N O T E these steps more than once. You may need to follow these steps if you change the USB port on the laptop for the TapeStation connector cable. 2200 TapeStation...

  • Page 23: Using The 2200 Tapestation System

    Aborting the TapeStation Run Complete TapeStation Run Empty Tip Buckets How to Use the ScreenPlex Analysis Software Shutdown and Restarting Procedure How to prepare your samples DNA Sample Preparation This chapter explains the intended use of the 2200 TapeStation System. Agilent Technologies...

  • Page 24: Intended Use Of The 2200 Tapestation System

    1 – 30 ng per fragment with a maximum concentration for one test sample of 75 ng. The sizing reproducibility for the same double stranded DNA species is within ±10 %. The 2200 TapeStation ScreenPlex System is intended for professional use only. Results obtained using this system must be interpreted in conjunction with other relevant data.

  • Page 25: Performance Limitations Of Use

    Using the 2200 TapeStation System Performance Limitations of Use Performance Limitations of Use The 2200 TapeStation Screenplex System can analyse a maximum of 15 samples at any one time. The user is responsible for establishing performance characteristics necessary for upstream and downstream applications. Appropriate controls must be...

  • Page 26: Components Required By The User

    Available tests • Thresholds required for peak detection • Fragment size ranges required for positive and negative readout) • 2200 TapeStation System (Agilent 2200 ScreenPlex Software) Components • ScreenTape DS12, Ladder and Loading Buffer (stains and separates amplified DNA) •...

  • Page 27: Operating Procedure

    Using the 2200 TapeStation System Operating Procedure Operating Procedure 1 Double click the 2200 TapeStation controller icon on the desktop and follow the instructions on the screen. Always ensure you are using the most up-to-date Controller software. Please check for the N O T E latest version.
  • Page 28 If any used tips are left in the tip-buckets, a pop up window will ask for the discarded tips to N O T E be removed. The 2200 TapeStation will not run until all the tip buckets are empty. A Loading tip transfer tool (G2964-60000) is available.
  • Page 29 39 or the appropriate Quick Guide. 8 Place samples into the sample block inside the TapeStation. Damage to the 2200 TapeStation and impact on performance C A U T I O N ➔ Ensure the lids have been removed from the sample tubes.
  • Page 30 Using the 2200 TapeStation System Operating Procedure The ladder must be selected first to ensure it's loaded into the first available lane. N O T E ScreenTape can be used up to 2 weeks after first use if it has been stored upright between N O T E 2 –...

  • Page 31: Add Experiment Notes

    Using the 2200 TapeStation System Operating Procedure Add Experiment Notes 1 If required, notes can be manually entered into the software before the instrument is started. This information will appear in the analysed results. N O T E 2200 TapeStation...

  • Page 32: Describe Samples

    Using the 2200 TapeStation System Operating Procedure Describe Samples 1 Sample descriptions can be manually entered into the software before the instrument is started and whilst the TapeStation is operating, before analysis software is launched. If samples are barcoded, place the cursor in the desired track description and scan the sample barcode with a hand-held barcode scanner.

  • Page 33: Start The Tapestation Run

    Using the 2200 TapeStation System Operating Procedure Start the TapeStation Run 1 Click the start button. This will produce a Save As window. As a default the file name starts with the date, in reverse order, and a run counter. When run continuously, the save function auto increments the counter part of the file name.

  • Page 34: Running System

    Using the 2200 TapeStation System Operating Procedure Running System Exposure to potentially dangerous mechanical parts WA R N I N G ➔ Do not open the lid whilst the light is flashing. Aborting the TapeStation Run 1 If, for any reason, you wish to abort an experiment, click the abort button on the pop-up controller.

  • Page 35: Empty Tip Buckets

    Using the 2200 TapeStation System Operating Procedure Empty Tip Buckets 1 Empty tip buckets. Used loading tips must be removed from the tip buckets before the next experimental run. N O T E The TapeStation will not start if tips are detected in the buckets.

  • Page 36: How To Use The Screenplex Analysis Software

    Using the 2200 TapeStation System Operating Procedure How to Use the ScreenPlex Analysis Software Once the imaging is complete, the analysis software will automatically launch the ScreenPlex Result File (extension .srf). The data provided includes the test types selected (displayed in tabs), the date and time at which the samples were run, the tape serial number and the system information.
  • Page 37 Using the 2200 TapeStation System Operating Procedure annotated with a warning sign on the image. If the internal control is undetected, a warning sign will illustrate the expected position on the gel image. • The review pane is accessed through Review in the top right hand corner of the ScreenPlex Result Viewer.

  • Page 38: Shutdown And Restarting Procedure

    Using the 2200 TapeStation System Operating Procedure • Clicking Reset All will remove all manual changes and return the entire tape to the automatically analysed state. • A text file, raw gel image (bitmap), Word and Excel report can all be exported and saved for each run.

  • Page 39: How To Prepare Your Samples

    Damage to the 2200 TapeStation instrument C A U T I O N ➔ Use only the recommended consumables and reagents with the 2200 TapeStation system. DNA ScreenTape, Ladders and Loading Buffers should be stored vertically between 2 – 8 °C N O T E in their original unopened packaging.
  • Page 40 For successful loading, the sample solution must be placed at the bottom of the tube or N O T E well without any air-bubbles. The 2200 TapeStation will load a sample from a minimum of 3 µL onto ScreenTape. 2200 TapeStation...

  • Page 41: Dna Sample Preparation

    The Ladder must be used with every instrument run and selected to load into the first N O T E available ScreenTape lane. Only Agilent Technologies provided ladder and loading buffers must be used with the N O T E Agilent 2200 TapeStation ScreenPlex System.
  • Page 42 Using the 2200 TapeStation System DNA Sample Preparation 2200 TapeStation...

  • Page 43: Maintenance

    2200 TapeStation Maintenance General Information Changing the Needle This chapter describes the maintenance of the TapeStation system. Agilent Technologies...

  • Page 44: General Information

    In addition to the above, the engineer will check that the instrument is functional by running a full ScreenTape. For customers with exceptionally high usage the needle replacement procedure as detailed in the section below can also be performed between annual PM services. 2200 TapeStation...

  • Page 45: Changing The Needle

    TapeStation for Nucleic acids Twin G2960-60063 ST010 TapeStation for Protein / Combined TapeStation G2960A 2200 TapeStation System G2961A 2200 TapeStation Nucleic Acid System G2964AA 2200 TapeStation System Twin G2960-60063 G2965AA 2200 TapeStation Nucleic Acid System G2966AA 2200 TapeStation ScreenPlex System...
  • Page 46 • After 4480 pierces (8960 lanes in a Dual loading system), the needle has completed its lifetime and must be changed before the TapeStation will start. Figure 5 Controller software indicating a Needle change is recommended 2200 TapeStation...
  • Page 47 Needle cartridge (for use in dual pump systems) For use with product numbers ST017, ST019, ST010, G2960A, G2961A, G2964AA, G2965AA and G2966AA New needles cartridges can be ordered at any time from Agilent Technologies by N O T E contacting your local sales agent.
  • Page 48 Maintenance Changing the Needle 2200 TapeStation...

  • Page 49: Troubleshooting

    2200 TapeStation Troubleshooting Agilent 2200 Controller Software Agilent 2200 ScreenPlex Software Error Messages ScreenTape Agilent 2200 TapeStation ScreenPlex Software Troubleshooting 2200 TapeStation ScreenPlex Images Troubleshooting the Test Kit Manufacturers TestKitSuite.xml File This chapter gives an overview on troubleshooting features. Agilent Technologies...

  • Page 50: Agilent 2200 Controller Software

    A ScreenTape has The barcode has not Power cycle the 2200 TapeStation and restart the been inserted but been scanned by the laptop. If the problem persists, contact the TestKit has not registered.
  • Page 51 The experimental The controller has Manually type in the desired experiment number – number has started been shut down and the 2200 TapeStation will then restart at 01 again. restarted. automatically incrementing. 2200 TapeStation...

  • Page 52: Agilent 2200 Screenplex Software Error Messages

    Agilent 2200 ScreenPlex Software Error Messages Message Probable cause Solution Please power cycle An error has Switch the 2200 TapeStation off and then back on the instrument occurred during the again. experimental run. Failed to connect to The drivers have not Power cycle the 2200 TapeStation.

  • Page 53: Screentape

    This may occur over Holding the tape with the ScreenTape label facing appeared in the time. you, flick the bottom of the tape. This will move buffer chamber of the any bubbles to the top of the chamber. tape. 2200 TapeStation...

  • Page 54: Agilent 2200 Tapestation

    2200 TapeStation before opening the lid. If the LED is constant, the lid can be opened as the 2200 TapeStation is idle. If the LED is flashing, do not open the lid as the 2200 TapeStation is running. Tips are stuck on The wrong tips may...

  • Page 55: Screenplex Software

    5 seconds and spun down the 2200 TapeStation optimally. before placing in the 2200 TapeStation. has provided a different result to the previous run. A completely blank There may be a...
  • Page 56 Ensure you check the top of the gel for bubbles sample fragment a bubble at the top of prior to running in the 2200 TapeStation. Any bands in the gel the gel. bubbles present at the top of the gel can easily be image appear slanted moved out of the way.

  • Page 57: Troubleshooting 2200 Tapestation Screenplex Images

    Only the top and Either the sample is Always vortex samples for 5 seconds before bottom markers are not present or is too placing in the 2200 TapeStation. ScreenTape can present in the faint to visualise. detect <1 ng/µL sample.
  • Page 58 The lane has not Always spin down samples before placing in the and/or ladder bands loaded optimally. 2200 TapeStation to remove any bubbles. appear very faint. Very rarely, the 2200 TapeStation does not load effectively - run the sample again. 2200 TapeStation...
  • Page 59 Troubleshooting Troubleshooting 2200 TapeStation ScreenPlex Images Table 8 Overview troubleshooting 2200 TapeStation images Image Issue Probable cause Solution A mark has appeared Dirt or specks of dust Deselect the annotated ghost band or run the lane in the lane which has can adhere to the again.

  • Page 60: Troubleshooting The Test Kit Manufacturers Testkitsuite.xml File

    Troubleshooting the Test Kit Manufacturers TestKitSuite.xml File Troubleshooting the Test Kit Manufacturers TestKitSuite.xml File Agilent Technologies accepts no responsibility for the contents of the TestKitSuite.xml file. N O T E Please contact the Test Kit Manufacturer for any troubleshooting advice that cannot be located below.
  • Page 61 The detection of any unexpected fragments that are sized out with these predetermined windows are marked as unidentified and presented with a warning sign in the gel and result table. 2200 TapeStation...
  • Page 62 Manufacturer. This is usually used for known non-specific have a pathogen amplification and/or primer dimers. marker sign next to them and are not reported in the result table. 2200 TapeStation...
  • Page 63 However, the internal control has not been Manufacturer. corresponding marked as unidentified. Therefore, it may have warning sign has been sized within an ignore region marked in the appeared next to a TestKitSuite and has not been reported fragment in the image. 2200 TapeStation...
  • Page 64 Troubleshooting Troubleshooting the Test Kit Manufacturers TestKitSuite.xml File 2200 TapeStation...

  • Page 65: Appendix

    2200 TapeStation Appendix Product Notices Sound Emission The Waste Electrical and Electronic Equipment Directive Technical Service This chapter provides addition information. Agilent Technologies...

  • Page 66: Product Notices

    The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer in conjunction with Agilent Technologies UK Limited’s automated gel electrophoresis system, where such research does not include testing, analysis or screening services for any third party in return for compensation on a per test basis.

  • Page 67: Sound Emission

    Sound Emission Directive of 18 January 1991. This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) 2200 TapeStation...

  • Page 68: The Waste Electrical And Electronic Equipment Directive

    With reference to the equipment types in the WEEE Directive Annex I, this product is classed as a Monitoring and Control Instrumentation product. Do not dispose off in domestic household waste N O T E To return unwanted products, contact your local Agilent office, or see www.agilent.com more information. 2200 TapeStation...

  • Page 69: Technical Service

    Appendix Technical Service Technical Service For more information, please contact Agilent Technologies UK Limited www.agilent.com/genomics/contact 2200 TapeStation...
  • Page 70 TapeStation WEEE directive electronic waste shutdown procedure software error messages intended use start up sound emission specifications limitations load screentape TapeStation tips abort complete set up needle start change 2200 TapeStation...
  • Page 72 In This Book The manual describes the following: • Introduction to the system • Specifications • Installation • Using the module • Maintenance • Addition information © Agilent Technologies 2011, 2012 Printed in Germany 08/2012 *G2966-90001* *G2966-90001* G2966-90001 Agilent Technologies...

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