Agilent Technologies 2200 TapeStation User Manual
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Agilent Technologies 2200 TapeStation User Manual

Agilent Technologies 2200 TapeStation User Manual

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Summary of Contents for Agilent Technologies 2200 TapeStation

  • Page 1 Agilent 2200 TapeStation User Manual Agilent Technologies...
  • Page 2 1987) or any equivalent agency regulation or contract clause. Use, duplication or dis- closure of Software is subject to Agilent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will Agilent 2200 TapeStation - User Manual...

  • Page 3: Table Of Contents

    Contents Contents 1 Introduction to the 2200 TapeStation System Overview of the System 2 Specifications Technical Specifications ScreenTape Specifications 3 Installing the System Unpacking the System Contents of the ScreenTape System Installing the System 4 Using the 2200 TapeStation System...
  • Page 4 Contents 6 Appendix Limited Use Label License Sound Emission The Waste Electrical and Electronic Equipment Directive Technical Service Agilent 2200 TapeStation - User Manual...

  • Page 5: Introduction To The 2200 Tapestation System

    Agilent 2200 TapeStation - User Manual Introduction to the 2200 TapeStation System Overview of the System ScreenTape Architecture Agilent 2200 TapeStation Components This chapter gives an introduction to the system Agilent Technologies...

  • Page 6: Overview Of The System

    2200 TapeStation) and an analysis software. The system is very straightforward to use, simply place the sample tubes and ScreenTape in to the 2200 TapeStation and let it load, separate, image, analyse and present the results. Place ScreenTape and some tips in the 2200 TapeStation.
  • Page 7 Introduction to the 2200 TapeStation System Overview of the System Information pertaining to sample and ScreenTape requirements can be found in: • ScreenTape Architecture • Operating Procedure ScreenTape Architecture Barcode: The unique barcode tracks lane usage within individual ScreenTape and allows traceability of results.

  • Page 8: Agilent 2200 Tapestation Components

    The USB connector is inserted into the USB socket to link the laptop to the 2200 TapeStation. Power-cable socket: The power cable must be connected to the 2200 TapeStation and the relevant mains electricity outlet. Agilent 2200 TapeStation - User Manual...
  • Page 9 Introduction to the 2200 TapeStation System Overview of the System ScreenTape Tip holder Sample block Figure 2 2200 TapeStation (front view) On/off switch Power-cable socket USB socket Figure 3 2200 TapeStation (back view) Agilent 2200 TapeStation - User Manual...
  • Page 10 Introduction to the 2200 TapeStation System Overview of the System Agilent 2200 TapeStation - User Manual...

  • Page 11: Specifications

    Agilent 2200 TapeStation - User Manual Specifications Technical Specifications ScreenTape Specifications This chapter provides information on specifications. Agilent Technologies...

  • Page 12: Technical Specifications

    14 – 30 °C (57 – 86 °F) for RNA and High Sensitivity RNA ScreenTape 10 – 33 °C (50 – 91 °F) for P200 ScreenTape Instrument operating temperature may be higher than ambient lab temperature, especially after prolonged use. Agilent 2200 TapeStation - User Manual...
  • Page 13 Check that any air conditioning is functioning. • If the quoted current temperature is below the specified range please allow the instrument to equilibrate to the ambient temperature, and avoid using in a cooled area. Agilent 2200 TapeStation - User Manual...

  • Page 14: Screentape Specifications

    96 samples: ≈100 min Samples per consumable Sample volume required 1 µL Kit stability 4 months Kit size 112 samples signal-to-noise >3 (single peak) Sizing Accuracy for software ladder: ±20% Measured against 2100 Bioanalyzer Agilent 2200 TapeStation - User Manual...
  • Page 15 96 samples: ≈100 min Samples per consumable Sample volume required 2 µL Kit stability 4 months Kit size 112 samples signal-to-noise >3 (single peak) Sizing Accuracy for software ladder: ±20% Measured against 2100 Bioanalyzer Agilent 2200 TapeStation - User Manual...
  • Page 16 96 samples: < 150 min Samples per consumable Sample Volume Required 1 µL Shelf Life 4 months Box/Kit size 112 samples/box Determined using the Genomic DNA Ladder as sample Average result from various genomic DNA sample types Agilent 2200 TapeStation - User Manual...
  • Page 17 16 samples < 16 min 96 samples < 100 min Samples per consumable Sample volume required (µL) Kit Stability 4 months Kit Size 112 samples For total RNA samples Within a ScreenTape Signal/noise >3 in water and TE Agilent 2200 TapeStation - User Manual...
  • Page 18 16 samples < 30 min 96 samples < 180 min Samples per consumable Sample volume required (µL) Kit Stability 4 months Kit Size 112 samples For total RNA samples Within a ScreenTape Signal/noise >3 in water and TE Agilent 2200 TapeStation - User Manual...
  • Page 19 Sensitivity Physical Specification Sample volume needed 2 µL Analysis Time 16 samples: <15 min Samples/consumable Kit Size 112 Samples Kit Stability 4 months determined using P200 Lader as sample signal :noise ratio > 3 Agilent 2200 TapeStation - User Manual...
  • Page 20 Specifications ScreenTape Specifications Agilent 2200 TapeStation - User Manual...

  • Page 21: Installing The System

    Agilent 2200 TapeStation - User Manual Installing the System Unpacking the System Contents of the ScreenTape System Installing the System Software Installation Agilent 2200 TapeStation Set Up This chapter gives information about how to install the system. Agilent Technologies...

  • Page 22: Unpacking The System

    Prerequisites conducted by an Agilent engineer. Instrument and software familiarization should be completed before use. Do not attempt to use the 2200 TapeStation instrument until you have read the accompanying Site and Safety Manual. Condensation within the instrument C A U T I O N Condensation will damage the system electronics.
  • Page 23 Delivery Checklist Ensure all parts and materials have been delivered with your system. The delivery checklist is shown below. Please report any missing or damaged parts to your local Agilent Technologies sales and service office. Agilent 2200 TapeStation - User Manual...

  • Page 24: Contents Of The Screentape System

    TapeStation • 2200 TapeStation System (G2964AA) or • 2200 TapeStation Nucleic Acid System (G2965AA) TapeStation Software 1x CD The software is required to drive the 2200 TapeStation and visualise the Setup Disc ScreenTape analysis Laptop 1x Laptop Instrument Control Laptop...
  • Page 25 Kit Components (Genomic DNA ScreenTape Assay) Part Number Name Color Amount 5067-5365 Genomic DNA ScreenTape 7 ScreenTape 5067-5366 Genomic DNA Reagents 2 vials • Genomic DNA Ladder 75 µL • Genomic DNA Sample Buffer 1350 µL Agilent 2200 TapeStation - User Manual...
  • Page 26 350 µL • P200 Reducing Sample Buffer 550 µL • P200 pH Buffer 1000 µL • P200 Non-Reducing Sample Buffer clear 550 µL • P200 Markers (pre-stained) 270 µL • P200 Ladder 40 µL Agilent 2200 TapeStation - User Manual...

  • Page 27: Installing The System

    Installing the System Installing the System Software Installation The software for your Agilent 2200 TapeStation system is preinstalled on the system laptop. For updates, or if you have to change the laptop, you may download the latest version of the N O T E software from the update server http://www.agilent.com/genomics/tapestation.

  • Page 28: Agilent 2200 Tapestation Set Up

    If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. For general safety information, please refer to the 2200 TapeStation System - Site and N O T E Safety Manual.
  • Page 29 As there is more than one driver that can be detected and installed, you may need to follow N O T E these steps more than once. You may need to follow these steps if you change the USB port on the laptop for the TapeStation connector cable. Agilent 2200 TapeStation - User Manual...
  • Page 30 Installing the System Installing the System Agilent 2200 TapeStation - User Manual...

  • Page 31: Using The 2200 Tapestation System

    Agilent 2200 TapeStation - User Manual Using the 2200 TapeStation System Intended Use of the 2200 TapeStation System Performance Limitations of Use Additional Components Required by the User Operating Procedure Add Experiment Notes Describe Samples Start the TapeStation Run Final Check...
  • Page 32 Using the 2200 TapeStation System Installing the System Protein Sample Preparation Sample Preparation P200 ScreenTape Assay This chapter explains the intended use of the 2200 TapeStation System. Agilent 2200 TapeStation - User Manual...

  • Page 33: Intended Use Of The 2200 Tapestation System

    Using the 2200 TapeStation System Intended Use of the 2200 TapeStation System Intended Use of the 2200 TapeStation System The 2200 TapeStation system (Agilent 2200 TapeStation Software) carries out electrophoretic separation of Nucleic Acids and proteins. The system detects: • Fluorescently stained double stranded DNA including genomic DNA •...

  • Page 34: Performance Limitations Of Use

    Performance Limitations of Use Performance Limitations of Use The 2200 TapeStation System (Agilent 2200 TapeStation Software) can analyse a maximum of 16 samples at any one time, more samples can be run using a 96- well plate and multiple ScreenTape.

  • Page 35: Additional Components Required By The User

    Using the 2200 TapeStation System Additional Components Required by the User Additional Components Required by the User Additional Consumables Required for the 2200 TapeStation Instrument • Loading tips (5067- 5152 or 5067- 5153) • Optical Tube 8x Strip (401428) and Optical Cap 8x Strip (401425) or 96- well Sample Plates (5067- 5150) and 96- well Plate Foil Seal (5067- 5154).

  • Page 36: Operating Procedure

    Using the 2200 TapeStation System Operating Procedure Operating Procedure 1 Double click the 2200 TapeStation controller icon on the desktop and follow the instructions on the screen. Always ensure you are using the most up-to-date Controller software. Please check for the N O T E latest version.
  • Page 37 If any used tips are left in the tip-buckets, a pop up window will ask for the discarded tips to N O T E be removed. The 2200 TapeStation will not run until all the tip buckets are empty. A Loading tip transfer tool (G2964-60000) is available.
  • Page 38 48 or the appropriate Quick Guide. 8 Place samples into the sample block inside the TapeStation. Damage to the 2200 TapeStation and impact on performance C A U T I O N ➔ Ensure the lids have been removed from the sample tubes.
  • Page 39 2 – 8 °C for a maximum of 2 weeks. DNA Reagents Store from 2 – 8 °C. RNA Sample Buffer Store from 2 – 8 °C. RNA Ladder Store below –20 °C. P200 Reagents Store from -30 to -20 °C. Agilent 2200 TapeStation - User Manual...
  • Page 40 • Clear All Selections - this will clear ladder well and all sample wells selected • Clear Last Selection - this will only clear the last samples to be highlighted Pressing Escape on the keyboard will also cancel the current selections. N O T E Agilent 2200 TapeStation - User Manual...

  • Page 41: Add Experiment Notes

    Using the 2200 TapeStation System Operating Procedure Add Experiment Notes 1 If required, notes can be manually entered into the software before the instrument is started. Agilent 2200 TapeStation - User Manual...

  • Page 42: Describe Samples

    Sample data can be copied and pasted from an Excel table. The entered Sample descriptions data can be copied to clipboard by using the Copy to N O T E Clipboard link in the top right hand corner of the Sample Description table. Agilent 2200 TapeStation - User Manual...
  • Page 43 In the 96-well plate sample selection screen the panel labeled LDR is not available for N O T E selection. All information entered in the control software will appear in the analysed results. N O T E Agilent 2200 TapeStation - User Manual...

  • Page 44: Start The Tapestation Run

    3 Close the lid. Lifting the lid of the TapeStation after this time will abort the experimental run. N O T E Agilent 2200 TapeStation - User Manual...

  • Page 45: Running System

    Aborting the experiment will irretrievably discard any progress made and samples loaded. N O T E Complete TapeStation Run When finished, a pop up will ask for removal of the tip cartridge and tape. 1 Remove tip cartridge and tape. 2 Click OK. Agilent 2200 TapeStation - User Manual...

  • Page 46: Empty Tip Buckets

    For further information please refer to the software help. N O T E This can be accessed by selecting the question mark (?) button in the top right hand corner of the TapeStation Analysis Software. Agilent 2200 TapeStation - User Manual...

  • Page 47: Shutdown And Restarting Procedure

    3 Power down the laptop. Restarting Procedure Ensure that the ScreenTape System is restarted in the following order: 1 Power up the laptop. 2 Turn on the TapeStation. 3 Start the TapeStation Controller Software. Agilent 2200 TapeStation - User Manual...

  • Page 48: How To Prepare Your Samples

    Damage to the 2200 TapeStation instrument C A U T I O N ➔ Use only the recommended consumables and reagents with the 2200 TapeStation system. • When pipetting sample buffer, ensure that excess buffer droplets are removed from the N O T E tip before transfer to the sample tubes.
  • Page 49 For successful loading, the sample solution must be placed at the bottom of the tube or N O T E well without any air-bubbles. The 2200 TapeStation will load a sample from a minimum of 3 µL onto ScreenTape. Ladder Options...

  • Page 50: Good Measurement Practices For Analysing Dna On The Agilent 2200 Tapestation

    Using the 2200 TapeStation System Good Measurement Practices for Analysing DNA on the Agilent 2200 TapeStation Good Measurement Practices for Analysing DNA on the Agilent 2200 TapeStation During assay preparation, the instructions regarding reagent preparation and instrument maintenance must be strictly followed. Important technical details are described in the TapeStation User Manual and other supporting documentation.

  • Page 51: Dna Sample Preparation

    Improper mixing can lead to quantification errors. As with samples in 8-way strips, briefly centrifuge after vortexing to collect the contents at the base of the tubes before placing into the TapeStation. Agilent 2200 TapeStation - User Manual...
  • Page 52 For successful loading, the sample solution must be placed at the bottom of the tube or N O T E well without any air-bubbles. The 2200 TapeStation will load a sample from a minimum of 3 µL onto ScreenTape. For best sizing precision and accuracy, run the appropriate ladder with the samples.

  • Page 53: Sample Preparation D1000 Screentape Assay

    Prepare TapeStation D1000 Parts required Description 5067-5582 D1000 ScreenTape 1 Launch the Agilent 2200 TapeStation software. 2 Load D1000 ScreenTape and loading tips into the 2200 TapeStation. Sample Preparation D1000 ScreenTape Assay Parts required Description 5067-5583 D1000 Reagents 1 Allow reagents to equilibrate at room temperature for 30 min 2 Vortex mix before use 3 If running ladder, add 3 µL D1000 Sample Buffer ( ) to 1 µL D1000...

  • Page 54: Sample Preparation High Sensitivity D1000 Screentape Assay

    Prepare TapeStation Parts required Description 5067-5584 High Sensitivity D1000 ScreenTape 1 Launch the Agilent 2200 TapeStation software. 2 Load High Sensitivity D1000 ScreenTape and loading tips into the 2200 TapeStation. Sample Preparation High Sensitivity D1000 ScreenTape Assay Parts required Description...

  • Page 55: Sample Preparation Genomic Dna Screentape Assay

    Sample Preparation Genomic DNA ScreenTape Assay Prepare TapeStation Parts required Description 5067-5365 Genomic DNA ScreenTape 1 Launch the Agilent 2200 TapeStation software. 2 Load Genomic DNA ScreenTape and loading tips into the 2200 TapeStation. Sample Preparation Genomic DNA ScreenTape Assay Parts required Description...
  • Page 56 Using the 2200 TapeStation System DNA Sample Preparation 4 Load sample plate, tips, ScreenTape and Samples into the TapeStation as detailed in operating procedure section. Agilent 2200 TapeStation - User Manual...

  • Page 57: Rna Sample Preparation

    • To ensure optimal performance of the RNA ScreenTape platform samples should be analysed, using the 2200 TapeStation, within 3 h of the denaturation step when left on the 2200 TapeStation system. Beyond 3 h, denatured samples should be stored on ice, or in a suitable freezable sample block.
  • Page 58 IKA for use with these assays. Please quote part number 4674100 when ordering. • Agilent Technologies will not sell these parts separately. • If an IKA MS3 vortexer is not available, please ensure thorough manual vortex mixing — 10 seconds on maximum speed.

  • Page 59: Sample Preparation Rna Screentape Assay

    Parts required Description 5067-5576 RNA ScreenTape 1 Launch the Agilent 2200 TapeStation software. 2 Load RNA ScreenTape and loading tips into the 2200 TapeStation. 3 Select RNA Protocol (Eukaryotic RNA or Prokaryotic RNA) Sample Preparation RNA ScreenTape Assay Parts required Description...
  • Page 60 RNA Sample Preparation 7 Sample denaturation a Heat samples to 72 °C for 3 min b Place samples on ice for 2 min c Centrifuge to collect samples in the base of the tubes Agilent 2200 TapeStation - User Manual...

  • Page 61: Sample Preparation High Sensitivity Rna Screentape Assay

    Parts required Description 5067-5579 High Sensitivity RNA ScreenTape 1 Launch the Agilent 2200 TapeStation software. 2 Load High Sensitivity RNA ScreenTape and loading tips into the 2200 TapeStation. 3 Select RNA Protocol (Eukaryotic RNA or Prokaryotic RNA) Sample Preparation High Sensitivity RNA ScreenTape Assay...
  • Page 62 7 Spin down to position the sample at the bottom of the tube. 8 Sample denaturation a Heat samples to 72 °C for 3 min b Place samples on ice for 2 min c Centrifuge to collect samples in the base of the tubes Agilent 2200 TapeStation - User Manual...

  • Page 63: Protein Sample Preparation

    For successful loading, the sample solution must be placed at the bottom of the tube or N O T E well without any air-bubbles. The 2200 TapeStation will load a sample from a minimum of 3 µL onto ScreenTape. For best sizing precision and accuracy, run the appropriate ladder with the samples.
  • Page 64 Prepare TapeStation Parts required Description 5067-5371 P200 ScreenTape 1 Launch the Agilent 2200 TapeStation software. 2 Load P200 ScreenTape and loading tips into the 2200 TapeStation. Sample Preparation P200 ScreenTape Assay Parts required Description 5067-5372 P200 Reagents 1 Prepare the P200 stain solution.
  • Page 65 Mix then heat at 75 °C for 5 min. d Remove condensation from the lids (or foil cover) of the tubes by centrifugation. 4 Add 2 µL of P200 Marker ( ) to each sample and to the P200 ladder. Agilent 2200 TapeStation - User Manual...
  • Page 66 TapeStation. Failure to do so may result in unsatisfactory analysis results. 6 Load sample plate, tips, ScreenTape and Samples into the TapeStation as detailed in operating procedure section. Agilent 2200 TapeStation - User Manual...

  • Page 67: Maintenance

    Agilent 2200 TapeStation - User Manual Maintenance General Information Changing the Needle Preventative Maintenance Interval This chapter describes the maintenance of the TapeStation system. Agilent Technologies...

  • Page 68: General Information

    Needle Change procedure every 8320 samples. This simple procedure is explained in the ‘Changing the needle’ section, and can be performed by the end user with the aid of a disposable cartridge. Agilent 2200 TapeStation - User Manual...

  • Page 69: Changing The Needle

    ST010 TapeStation for Protein / Combined TapeStation G2960A 2200 TapeStation System G2961A 2200 TapeStation Nucleic Acid System G2964AA 2200 TapeStation System Twin G2960-60063 G2965AA 2200 TapeStation Nucleic Acid System G2966AA 2200 TapeStation ScreenPlex System Agilent 2200 TapeStation - User Manual...
  • Page 70 Needle cartridge (for use in single pump systems) For use with product numbers ST007, ST008 and ST009 G2960-60063 Needle cartridge (for use in dual pump systems) For use with product numbers ST017, ST019, ST010, G2960A, G2961A, G2964AA, G2965AA and G2966AA Agilent 2200 TapeStation - User Manual...
  • Page 71 Customers with high throughput may require additional needle changes between PM services, and should use the table below to order the correct parts for their instrument. New needles cartridges can be ordered at any time from Agilent Technologies by N O T E contacting your local sales agent.

  • Page 72: Preventative Maintenance Interval

    The Preventative Maintenance procedure includes a Needle Change and following PM service both messages will disappear. Figure 9 Controller software indicating a Preventative Maintenance is due. Schedule Preventative Maintenance, do not perform a Needle Change Agilent 2200 TapeStation - User Manual...

  • Page 73: Appendix

    Agilent 2200 TapeStation - User Manual Appendix Limited Use Label License Sound Emission The Waste Electrical and Electronic Equipment Directive Technical Service This chapter provides addition information. Agilent Technologies...

  • Page 74: Limited Use Label License

    Molecular Probes, Inc. (a wholly owned subsidiary of Life Technologies Corporation) and Agilent Technologies UK Limited, and the manufacture, use, sale or import of this product is subject to one or more of U.S. Patents and corresponding international equivalents, owned by Molecular Probes, Inc.

  • Page 75: Sound Emission

    This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) Agilent 2200 TapeStation - User Manual...

  • Page 76: The Waste Electrical And Electronic Equipment Directive

    Monitoring and Control Instrumentation product. Do not dispose of in domestic household waste N O T E To return unwanted products, contact your local Agilent office, or see www.agilent.com more information. Agilent 2200 TapeStation - User Manual...

  • Page 77: Technical Service

    Appendix Technical Service Technical Service For more information, please contact Agilent Technologies UK Limited www.agilent.com/genomics/contact Agilent 2200 TapeStation - User Manual...
  • Page 78 TapeStation shutdown electronic waste procedure software start up sound emission good measurement practice specifications ScreenTape limitations TapeStation load abort screentape complete tips set up start unpacking needle change technical service notes Agilent 2200 TapeStation - User Manual...
  • Page 79 Index Agilent 2200 TapeStation - User Manual...
  • Page 80 The manual describes the following: • Introduction to the system • Site requirements and specifications • Installation • Using the system • Maintenance • Product notices © Agilent Technologies 2011-2013, 2014 Printed in Germany 10/2014 *G2964-90003* *G2964-90003* G2964-90003 Agilent Technologies...

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