Applying The Sample - GE AKTA avant Operating Instructions Manual

6.2

Applying the sample

Introduction
A number of different sample application techniques are available. The sample can be
applied either directly onto the column using the sample pump, or via a loop. A loop can
be filled either manually or by using the sample pump.
This section describes sample application using a syringe to manually fill a sample loop.
Sample application stages
The two stages of the sample application are described in the following table. For detailed
instructions and information regarding the different sample applications techniques,
see ÄKTA avant User Manual.
Stage
Load
Inject
How to fill a sample loop
Follow the instructions to fill the sample loop with sample.
Step
1
2
ÄKTA avant Operating Instructions 29101556 AB
Description
The sample loop is filled with sample.
The sample is injected onto the column.
Action
Connect a suitable sample loop to the Injection Valve ports LoopF (fill) and
LoopE (empty).
Fill a syringe with sample.
6 Run a method

6.2 Applying the sample

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