Prime Sample Inlets And Purge Sample Pump - GE AKTA avant Operating Instructions Manual

Preparative chromatography system
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4.5.2

Prime sample inlets and purge Sample Pump

Overview
The procedure consists of the following stages:
Stage
1
2
3
4
5
Note:
Prime sample inlets
Follow the instructions below to fill all sample inlet tubing, to be used in the run, with
appropriate buffer or sample solution.
Step
1
2
3
ÄKTA avant Operating Instructions 29101556 AB
Description
Prime all sample inlet tubing to be used during the run.
Validate priming of inlet tubing.
Purge the sample pump if pressure signal indicates air bubbles.
Validate purge of the samle pump.
End the run.
To increase life length of the pump sealing rings, make sure that the pump
rinsing system is filled with fresh rinsing solution.
Action
Make sure that all sample inlet tubing that is to be used during the method
run is immersed in the correct samples or buffer.
Make sure that the waste tubing connected to injection valve port W2 is
immersed in a waste vessel.
Open the System Control module.
4.5 Prime inlets and purge pump heads
4.5.2 Prime sample inlets and purge Sample Pump
4 Installation
93

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