Applying The Sample - GE ÄKTA pure Operating Instructions Manual

6.2

Applying the sample

Introduction
A number of different sample application techniques are available. This section describes
sample application using a syringe to manually fill a s loop. The two stages of the sample
application are described in the table below. For detailed instructions and information
regarding the different sample applications techniques, see ÄKTA pure System Handbook.
Stage
Load
Inject
How to fill a sample loop
Follow the instructions below to fill the sample loop with sample.
Step
1
2
ÄKTA pure Operating Instructions 29-0229-97 AE
Description
The sample loop is filled with sample.
The sample is injected onto the column.
Action
Connect a suitable sample loop to the injection valve ports LoopF (fill) and
LoopE (empty).
Fill a syringe with sample.
6 Run a method

6.2 Applying the sample

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