Prepare Sample For Loading - GE AKTA GO Operating Instructions Manual

6 Run a method

6.2 Prepare sample for loading

6.2

Prepare sample for loading

Introduction
This section describes how to prepare the sample for loading onto the column. This can
be done using either a sample loop, a Superloop, or the pump. When using the pump to
apply the sample, the sample inlet must first be primed.
Sample application technique
Sample loop
Superloop
Pump
Prepare sample for application
using a sample loop
Follow the steps below to prepare for sample application using a sample loop.
Step
1
2
3
4
5
82
Action
Connect a suitable sample loop to the injection valve ports LoopF (fill) and
LoopE (empty).
Fill a syringe with sample.
Connect the syringe to the injection valve port Syr.
In the Process Picture, make sure that the injection valve is in position Load.
Note:
Load is the default position for the valve.
Load sample into the sample loop. To avoid sample loss due to siphoning,
leave the syringe in the port until the sample has been injected onto the
column during the run.
Tip:
When repeatability is important overload the loop with 3 to 5 volumes of
sample to make sure that the loop is completely filled.
Note:
Make sure the waste bottle is located below the injection valve, to avoid waste
going back into the sample loop.
Suitable volume
25 μL to 10 mL
1 mL to 150 mL
From 5 mL
ÄKTA go Operating Instructions 29360951 AC