GE AKTA avant Operating Instructions Manual page 105

ÄKTA avant Operating Instructions 29101556 AB
WARNING
When using flammable liquids with the ÄKTA avant instrument,
follow these precautions to avoid any risk of fire or explosion.
•
Fraction collector. Do not fractionate flammable liquids in the
built-in fraction collector. When running RPC methods, collect
fractions through the outlet valve or the optional external
Fraction collector F9-R.
•
RPC runs with 100% acetonitrile and system pressure above
5 MPa (50 bar) in ÄKTA avant 25. Always replace the green
PEEK tubing between the used system pump and the pump
pressure monitor with orange PEEK tubing, i.d. 0.5 mm, before
running RPC with 100% acetonitrile. Set the system pressure
alarm to 10 MPa (100 bar).
•
RPC runs with 100% acetonitrile in ÄKTA avant 150. Always
replace the beige PEEK tubing between the used system pump
and the pump pressure monitor before running RPC with 100%
acetonitrile. Replace it with green PEEK tubing, i.d. 0.75 mm.
5 Prepare the system for a run
5.1 Before you prepare the system
105