Set Pressure Alarms - GE AKTA avant Operating Instructions Manual

5.5

Set pressure alarms

Introduction
The columns can be protected by two different types of pressure alarms:
•
•
The column valves V9-C and V9H-C have built-in pressure sensors that automatically
measure the pre-column and delta-column pressure.
See the instructions in the next topic to set the pressure alarm for the column to be used
in the run and, if applicable, to set the parameters for the tubing dimensions.
Note:
Pre-column pressure alarms
It is important that the pre-column pressure alarm is set during all runs where a column
is used. The pressure alarm can be set in: the method to be run, the System Settings
dialog box, or during a manual run.
Pre-column pressure alarm limits are automatically set in the method when a column
from the column list is selected in the method. Refer to UNICORN Method Manual for
more information on pressure alarms.

Set pressure alarms

Pressure alarm limits may be set manually in System Control. The example below de-
scribes how to set the high pressure limit for the column. Other alarms are set in a cor-
responding way.
ÄKTA avant Operating Instructions 29101556 AB
The pre-column pressure alarm protects the column hardware.
The delta-column pressure alarm protects the column media.
Remember to lower the system pressure alarm and sample pressure alarm if
the optional UV Monitor U9-L and/or the optional second Conductivity Monitor
C9 is used on the high pressure side in the system (before the column[s]).
NOTICE
UV and conductivity flow cells on the high pressure side. When
placing UV and/or conductivity flow cells on the high pressure side
of the column, the UV flow cell has a maximum pressure limit of 2
MPa (20 bar) and the conductivity flow cell has a maximum pressure
limit of 5 MPa (50 bar).
5 Prepare the system for a run
5.5 Set pressure alarms
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