Perform A Run - GE AKTA avant User Manual

5 Operation
5.9 Create a method and perform a run

5.9.2 Perform a run

5.9.2 Perform a run
Checklist
Make sure that the system is correctly prepared. Check that:
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The system is prepared according to Section 5.1 Before you prepare the system, on
page 156.
A suitable column has been selected for the application. Consider target protein and
pressure range.
The buffer inlet tubing is immersed in correct buffer vessels. Consider solution iden-
tity and volume.
Note:
Inlet A1 and B1 must always be immersed in buffer or water. When the
System pumps are synchronized, the Inlet valves are positioned to A1 and
B1 for a short moment. If the Sample pump is to be used, inlet Buff must
be immersed in buffer or water.
The waste tubing is immersed into appropriate waste vessels. Consider vessel size
and vessel material.
No tubing is twisted and the flow path is free from leakage.
Check the pump piston rinsing system. Check that liquid is coming out of the outlet
tubing and that each tube contains at least 25 ml. If not, add liquid. Evaporation can
decrease volumes. To fill and prime the system, see Section 7.3.1 Change pump
rinsing solution, on page 273.
CAUTION
Close doors. To minimize the risk of exposure to hazardous
chemicals and pressurized liquids, always close the foldable door
and the pump cover before starting a run.
WARNING
High pressure. The product operates under high pressure. Wear
protective glasses and other required Personal Protective Equip-
ment (PPE) at all times.
ÄKTA avant User Manual 29035184 AE