Manual Instructions - Monitors - GE AKTA avant User Manual

9.8.3

Manual instructions - Monitors

The table below describes the monitor related manual instructions available for ÄKTA
avant.
Instruction name
Auto zero UV
Auto zero UV 2nd
Wavelength
Noise reduction UV
Noise reduction UV 2nd
Relative scale cond
ÄKTA avant User Manual 29035184 AE
Description
Sets the UV signals from U9-M to 0 mAU.
Sets the UV signal from U9-L, 2nd to 0 mAU.
Sets the wavelengths for UV monitor U9-M. The corre-
sponding curves in the chromatogram are marked with
the set wavelengths.
Note:
•
Do not use more wavelengths than necessary for the
application.
•
At low wavelengths, the eluent can have absorbance
of its own.
Filters the noise in the UV signal from UV monitor U9-M.
A column-specific averaging time is set automatically
when a column is defined in a method run and Averag-
ing time is set as a variable.
Filters the noise in the UV monitor signal from U9-L, 2nd.
A column-specific averaging time is set automatically
when a column is defined in a method run and Averag-
ing time is set as a variable.
Facilitates monitoring using the conductivity monitor,
of a gradient, for which the user sets the conductivity
values for 0% and 100%. The Relative scale cond in-
struction can be set in ascending manner (0% for low
and 100% for high conductivity) or in descending man-
ner (0% for high and 100% for low conductivity).
Note:
The Relative scale cond instruction in descending man-
ner is especially useful for conductivity visualization in
RPC and HIC, where the conductivity curve is reversed
compared to the concentration curve (i.e., high conduc-
tivity at 0% B and low conductivity at 100% B).
9 Reference information
9.8 Using Manual instructions
9.8.3 Manual instructions - Monitors
481