Manual Instructions - Pumps And Pressures - GE AKTA avant User Manual

9 Reference information
9.8 Using Manual instructions

9.8.1 Manual instructions - Pumps and pressures

9.8.1 Manual instructions - Pumps and pressures
The following table describes the pump and related manual instructions available for
ÄKTA avant.
Instruction name
System flow
Sample flow
Gradient
Pump wash
476
Description
Defines the system flow rate.
Flow rate can be set either as volumetric or as linear
flow. A column type must be selected before using linear
flow.
Defines the system flow rate. Flow rate can be set either
as volumetric or as linear flow. A column type must be
selected before using linear flow.
Sets a gradient (linear or stepwise) using the system
pumps A and B.
Note:
Set gradient length value to 0 to perform a step gradient.
Is used to change buffers in the inlet tubing, pump and
mixer.
Note:
•
Pressing End during Pump wash will terminate both
the wash and the run immediately.
Pressing Continue during Pump wash will terminate
the wash and the run will continue from the point at
which the Pump wash instruction was executed.
•
An instruction issued when a Pump wash is in
progress will not be executed until the wash is com-
pletely finished and all valves have turned back to
the previous positions.
•
Pump wash cannot be executed when the system
is in state HOLD.
ÄKTA avant User Manual 29035184 AE