Performing A Run - GE AKTAprime plus Operating Instructions Manual

1 Connect a sample loop between port 2 and 6 on the injection valve. Make sure that
2 Connect a luer female/1/16" male union to port 3.
3 Fill a syringe with five loop volumes of deionized water or binding buffer.
4 Fit the syringe in the Luer union (1) and carefully inject the buffer.
5 Remove the syringe and fill it with at least two loop volumes of the sample.
6 Carefully inject the sample into the sample loop. Do NOT remove the syringe after
4.4

Performing a run

Selecting template and starting
the run
1 Select Templates in the main menu and press OK.
2 Select Application Template and press OK.
3 Select the appropriate template, for example His Tag Purification HisTrap, and press
4 Set the sample volume and press OK.
5 Press OK to start the purification run.
ÄKTAprime plus Operating Instructions 28-9597-89 AB
the sample loop is large enough for your sample.
the injection because the loop might otherwise be emptied due to self-drainage or
air may be introduced in the flow path.
OK.
1
3
4 Operation
4.3 Preparations before start
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