Fractionation Overview - GE AKTA avant User Manual

5 Operation
5.8 Fractionation

5.8.3 Fractionation overview

5.8.3 Fractionation overview
Fractionation types
The following table lists the types of fractionation that the fraction collectors can be
used for.
Type
Fixed volume frac-
tionation
Peak fractionation
Combined fixed
volume fractiona-
tion and peak frac-
tionation
To be able to analyze different parts of the peak, the fraction size during elution is usually
set to a value smaller than the expected peak volume.
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Description
During fixed volume fractionation the fraction collector contin-
uously switches tubes according to the set volume throughout
the entire fractionation. This type of fractionation is also known
as straight fractionation.
Peak fractionation can be used to further increase the purity
of the collected protein peaks and minimize the number of
tubes used. The monitor signal is used to determine when to
switch the tubes. See Section 9.7.8 System settings - Watch
parameters, on page 471 for information about different watch
options.
The two fractionation types listed above can be used in combi-
nation. Combination of fixed volume and peak fractionation
allows fractions collected by fixed volume fractionation and
fractions collected by peak fractionation to be directed to dif-
ferent collection tubes.
ÄKTA avant User Manual 29035184 AE