Beckman Coulter EPICS XL-MCL Troubleshooting Manual
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Beckman Coulter EPICS XL-MCL Troubleshooting Manual

Beckman Coulter EPICS XL-MCL Troubleshooting Manual

Flow cytometer
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COULTER® EPICS® XL™ Flow Cytometer
COULTER EPICS XL-MCL™ Flow Cytometer
SYSTEM II™ Software
PN 4237296CA (September 2010)
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821
CYTOMETER
READY
FS
SS
LASER
ON
AUX
FL1
SAMPLE FLOW
FL2
LOW
MED HIGH
FL3
FL4
®
TM
Special Procedures
and Troubleshooting

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  • Page 1 COULTER® EPICS® XL™ Flow Cytometer COULTER EPICS XL-MCL™ Flow Cytometer SYSTEM II™ Software Special Procedures and Troubleshooting CYTOMETER READY LASER SAMPLE FLOW MED HIGH ® PN 4237296CA (September 2010) Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821...
  • Page 2 This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals. r You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your system’s computer with software authorized by Beckman Coulter.

  • Page 3: Revision Status

    This document applies to the latest software listed and higher versions. When a subsequent software version changes the information in this document, a new issue will be released to the Beckman Coulter website. For labeling updates, go to www.beckmancoulter.com and download the most recent manual or system help for your instrument.
  • Page 4 REVISION STATUS PN 4237296CA...

  • Page 5: Table Of Contents

    CONTENTS WARNINGS AND PRECAUTIONS, ii REVISION STATUS, iii INTRODUCTION, ix USING YOUR XL AND XL-MCL FLOW CYTOMETER MANUALS, ix ABOUT THIS MANUAL, ix CONVENTIONS, x ICONS, x CLEANING PROCEDURES, 1-1 WHAT THIS CHAPTER EXPLAINS, 1-1 CLEANING THE AIR FILTERS, 1-1 Prepare to Clean the Air Filters, 1-2 Rinse and Return the Air...
  • Page 6 CONTENTS REPLACING REAGENTS, 2-1 About the Reagent Containers, 2-1 FILLING THE SHEATH CONTAINER, 2-2 VENTING AIR BUBBLES, 2-5 FILLING THE CLEANING AGENT CONTAINER, 2-7 EMPTYING THE WASTE CONTAINER, 2-10 REPLACING THE SHEATH FILTER, 2-13 REPLACING THE MANUAL SAMPLE HEAD, 2-15 ADJUSTING THE SYSTEM PRESSURE, 2-18 2.10 POSITIONING THE NEUTRAL DENSITY...
  • Page 7 CONTENTS Making Or Deleting An Entry, B-4 CHANGING THE PASSWORD (SUPERVISOR LEVEL), B-5 INDEX, INDEX-1 TRADEMARKS PN 4237296CA...
  • Page 8 CONTENTS PN 4237296CA viii...

  • Page 9: Introduction

    INTRODUCTION This introductory section contains the following topics: Using your COULTER EPICS XL and XL-MCL Flow Cytometer manuals About this Manual Conventions Icons. USING YOUR XL AND XL-MCL FLOW CYTOMETER MANUALS Use the Reference manual for in-depth information on the principles of flow cytometry, information about what your instrument does, the methods it uses, its specifications, and information on installation, safety, and system options.

  • Page 10: Conventions

    INTRODUCTION CONVENTIONS Bar-Code Options QC Maintenance Screen Index Use the Index to easily locate specific information in this manual. CONVENTIONS This manual uses the following conventions: Throughout this manual your EPICS XL or XL-MCL flow cytometer is referred to as the system.
  • Page 11 INTRODUCTION ICONS PN 4237296CA...
  • Page 12 INTRODUCTION ICONS PN 4237296CA...

  • Page 13: Cleaning Procedures

    CLEANING PROCEDURES WHAT THIS CHAPTER EXPLAINS This chapter explains how to clean the: Air filters (weekly) after the shutdown procedure Sheath fluid container (monthly) and cleaning agent container (every 60 days) VACuum TRAP (VAC TRAP) on the Power Supply (as needed). For daily cleaning procedures, see the Shutdown chapter in the Operator's Guide.

  • Page 14: Prepare To Clean The Air Filters

    CLEANING PROCEDURES CLEANING THE AIR FILTERS Prepare to Clean the Air Filters Turn off the system at the computer. Pull off each filter cover. Even though the covers look like they are screwed in, they are not. The filter covers are made of flexible plastic;...

  • Page 15: Rinse And Return The Air Filters

    CLEANING PROCEDURES CLEANING THE AIR FILTERS Rinse and Return the Air Filters Rinse each filter in water b, and then wring it out c. 7027029C Set each filter aside and let it dry out for about 30 minutes. Use paper towels to check that each filter is completely dry.

  • Page 16: Idle Mode,

    CLEANING PROCEDURES IDLE MODE Turn on the system at the computer. Record that the air filters were cleaned on the QC Maintenance screen. See Appendix B for instructions. Do you want to use the instrument? Do the startup procedure You are in the Operator’...

  • Page 17: Mode

    CLEANING PROCEDURES REMOVING THE REAGENT CONTAINERS REMOVING THE REAGENT CONTAINERS Remove and clean the sheath fluid container once a month. Remove and clean the cleaning agent container every 60 days. Clean the reagent containers when the instrument indicates that the sheath fluid or cleaning agent is low, before you refill them.
  • Page 18 CLEANING PROCEDURES REMOVING THE REAGENT CONTAINERS Disconnect the tubing on the top of the container by pushing in on the metal clips on the connectors. 7027095C Pull open the reagent drawer all the way so you can disconnect the sensor at the back of the drawer by sliding its sleeve out.

  • Page 19: Cleaning The Sheath Container

    CLEANING PROCEDURES CLEANING THE SHEATH CONTAINER CLEANING THE SHEATH CONTAINER IMPORTANT Misleading results could occur if you contaminate the sheath fluid container. Be careful not to contaminate the sheath fluid container. Do not let your fingers, paper towels, or other objects touch the inside of the container or the inside of its cap.
  • Page 20 CLEANING PROCEDURES CLEANING THE SHEATH CONTAINER Empty the container as completely as possible. 7027110C Replace the sheath container as instructed in Heading 1.7, Replacing the Reagent Containers. Record that the sheath fluid container was cleaned on the QC Maintenance screen. See Appendix B for instructions.
  • Page 21 CLEANING PROCEDURES CLEANING THE CLEANING AGENT CONTAINER CLEANING THE CLEANING AGENT CONTAINER Remove and clean the cleaning agent container every 60 days. Follow the steps in Heading, 1.4, Removing The Reagent Containers, and then: Fill the cleaning agent container with 50 to 100 mL of fresh IsoFlow 7027111C sheath fluid.
  • Page 22 CLEANING PROCEDURES CLEANING THE CLEANING AGENT CONTAINER Fill the container with 50 to 100 mL of fresh COULTER CLENZ® cleaning agent. (Do not use any other type of cleaning agent.) 7027114C Screw the cap back on and then swirl the cleaning agent in the container, rinsing all surfaces.

  • Page 23: Replacing The Reagent Containers

    CLEANING PROCEDURES REPLACING THE REAGENT CONTAINERS Record that the cleaning agent container was cleaned on the QC Maintenance screen. See Appendix B for instructions. REPLACING THE REAGENT CONTAINERS Put the container back in the drawer. Check that the connector at the back of the container goes through the hole in the back of the drawer, and then reconnect the...

  • Page 24: Cleaning The Vacuum Trap

    CLEANING PROCEDURES CLEANING THE VACUUM TRAP CLEANING THE VACUUM TRAP Clean the vacuum trap as needed. Check for fluid in the VACuum TRAP (VAC TRAP) on the front of the Power Supply as part of your daily startup procedure. If the VAC TRAP is more than one-quarter full of fluid, empty and rinse FILTER it with tap water.

  • Page 25: Filter,

    CLEANING PROCEDURES CLEANING THE VACUUM TRAP Turn off the system at the back of the Power Supply. SYSTEM WASTE ON / OFF PRESSURE LEVEL / PRESS VACUUM WASTE LASER SYSTEM POWER POWER WASTE VENT 7027021C 1-13 PN 4237296CA...

  • Page 26: Find And Pull Out Vac Trap

    CLEANING PROCEDURES CLEANING THE VACUUM TRAP Find and Pull Out VAC TRAP The VAC TRAP is the trap on the left. Lift the VAC TRAP assembly out of its bracket so that you can grasp the top of the assembly. 7027065C WARNING To prevent injury, avoid skin contact with the VAC TRAP and its associated tubing.

  • Page 27: Rinse And Return Vac Trap To Its Bracket

    CLEANING PROCEDURES CLEANING THE VACUUM TRAP Rinse and Return VAC TRAP to its Bracket Rinse the VAC TRAP with water, and then shake out the excess water. 7027067C Insert the white center post, pointed end up, into the VAC TRAP assembly.
  • Page 28 CLEANING PROCEDURES CLEANING THE VACUUM TRAP Carefully align the threads on the VAC TRAP jar with the threads on the VAC TRAP assembly and screw the VAC TRAP back into place. 7027066C Return the VAC TRAP assembly to its bracket. 7027069C Wipe up any spills.
  • Page 29 CLEANING PROCEDURES CLEANING THE VACUUM TRAP Turn on the system at the Power Supply. SYSTEM WASTE PRESSURE ON / OFF LEVEL / PRESS VACUUM WASTE LASER SYSTEM POWER POWER WASTE VENT 7027028C Check that no error messages are displayed. If there are, see Heading 3.3, Error Messages.

  • Page 30: Cleaning The Sampling System

    CLEANING PROCEDURES CLEANING THE SAMPLING SYSTEM CLEANING THE SAMPLING SYSTEM When to Clean the Sampling System Routine daily cleaning helps to minimize instrument downtime. Follow both Manual and Auto mode procedures for the XL-MCL flow cytometer. For the XL flow cytometer, follow only the Manual mode procedures. The two levels of cleaning for the system are: Routine cleaning followed by sample head cleaning.

  • Page 31: Cytometers),

    CLEANING PROCEDURES CLEANING THE SAMPLING SYSTEM Manual Mode Procedure (XL and XL-MCL Flow Cytometers) Prepare a cleaning solution of 1 part high-quality, fragrance-free bleach (5% solution of sodium hypochlorite - available chlorine) and 9 parts distilled water or IsoFlow sheath fluid. Put 2 mL of the bleach solution in a test tube.
  • Page 32 CLEANING PROCEDURES CLEANING THE SAMPLING SYSTEM When the sample stage lowers, remove the tube. 7021203A Run three tubes of distilled water or About 2 mL of distilled water IsoFlow sheath fluid. or sheath fluid in each 7296004B Record that the routine cleaning was done on the QC Maintenance screen.

  • Page 33: Only),

    CLEANING PROCEDURES CLEANING THE SAMPLING SYSTEM Auto Mode Procedure (XL-MCL Flow Cytometer Only) Put a test tube containing 2 mL of freshly prepared bleach solution in carousel position 1. Then put three freshly prepared tubes, each containing about 2 mL of distilled water or IsoFlow sheath fluid, in positions 2, 3, and 4 of the carousel.

  • Page 34: Procedure

    CLEANING PROCEDURES CLEANING THE SAMPLING SYSTEM Testing for Residual Stain If you use vital stains such as propidium iodide, ethidium bromide, acridine orange, thiazole orange, Coriphosphine-O, Fura 3, or fluorescein diacetate, you may want to test for residual stain after performing the routine cleaning procedure and before proceeding to your next application.

  • Page 35: Auto Mode Procedure

    CLEANING PROCEDURES CLEANING THE SAMPLING SYSTEM Auto Mode Procedure (XL-MCL Flow Cytometer Only) Prepare a cleaning solution of 1 part of high-quality, fragrance-free bleach (5% solution of sodium hypochlorite - available chlorine) and 9 parts of distilled water or IsoFlow sheath fluid. While wearing latex gloves, apply the 10% bleach solution to a gauze pad.

  • Page 36: Vacuum Line Cleaning Procedure

    CLEANING PROCEDURES CLEANING THE SAMPLING SYSTEM Vacuum Line Cleaning Procedure Auto Mode Procedure (XL-MCL Flow Cytometer Only) Fill two cleaning adaptors with 5 mL of distilled water. 7021271B Put one adaptor in position 1 of a Adaptor with distilled water carousel.

  • Page 37: Cytometers),

    CLEANING PROCEDURES CLEANING THE SAMPLING SYSTEM When Press Run to Initialize appears on the status line, remove the carousel. Record that the vacuum line cleaning and cleanse cycle were done on the QC Maintenance screen. See Appendix B for instructions. Manual Mode Procedure (XL and XL-MCL Flow Cytometers) If the system is in the Run mode (the RUN button is glowing green),...
  • Page 38 CLEANING PROCEDURES CLEANING THE SAMPLING SYSTEM Remove the adaptor and press the CLEANSE button. When the cleanse cycle ends, the CLEANSE button indicator turns off and the RUN button indicator flashes. The Cytometer status message reads Press Run to Initialize. 7021-056 Record that the vacuum line cleaning and cleanse cycle were...

  • Page 39: Procedures

    REPLACE/ADJUST PROCEDURES WHAT THIS CHAPTER EXPLAINS This chapter has these replacement and adjustment procedures: Filling the sheath container Venting air bubbles Filling the cleaning agent container Emptying the waste container•Replacing the sheath filter Replacing the sample head Adjusting the system pressure Positioning the neutral density filter.

  • Page 40: Filling The Sheath Container

    REPLACE/ADJUST PROCEDURES FILLING THE SHEATH CONTAINER FILLING THE SHEATH CONTAINER Is the instrument in the Idle mode? Go to Press the RUN button. step 2. The indicator in the button flashes green. Wait about 10 seconds for the Cytometer to depressurize.
  • Page 41 REPLACE/ADJUST PROCEDURES FILLING THE SHEATH CONTAINER CAUTION To prevent damage to the instrument, do not overfill the sheath container. Avoid spills. Do not tilt the container or remove it from the drawer to fill it. Carefully pour fluid into the container, filling it just to the bottom of its neck.
  • Page 42 REPLACE/ADJUST PROCEDURES FILLING THE SHEATH CONTAINER Are bubbles in the vent tubing? Go to Close the reagent Heading 2.4, drawer, and press Venting Air the RUN button. Bubbles The indicator in the button glows (not glashes) green. Do the quality control procedure in the Startup chapter of the Operator’...

  • Page 43: Venting Air Bubbles

    REPLACE/ADJUST PROCEDURES VENTING AIR BUBBLES VENTING AIR BUBBLES The vent button lets you release bubbles that are in the sheath filter and in the tubing for the sheath fluid. While the reagent drawer is open, the vent button in the drawer is deactivated. This means that you cannot use the vent button unless the drawer is closed and the Cytometer is in the Run or Prime...
  • Page 44 REPLACE/ADJUST PROCEDURES VENTING AIR BUBBLES Remove the Cytometer center front panel. Put the system in the Run or Prime mode. 7027031C Press and hold the vent button until the bubbles in the vent tubing are O.K. gone.‘ Reposition the center front panel. It snaps into place.

  • Page 45: Filling The Cleaning Agent Container

    REPLACE/ADJUST PROCEDURES FILLING THE CLEANING AGENT CONTAINER FILLING THE CLEANING AGENT CONTAINER Is the instrument in the Idle mode? Go to Press the RUN button. step 2. The indicator in the button flashes green. Wait about 10 seconds for the Cytometer to depressurize.
  • Page 46 REPLACE/ADJUST PROCEDURES FILLING THE CLEANING AGENT CONTAINER CAUTION To prevent damage to the instrument do not overfill the cleaning agent container. Avoid spills. Do not tilt the container or remove it from the drawer to fill it. Carefully pour cleaning agent into the container, filling it just to the bottom of its neck.
  • Page 47 REPLACE/ADJUST PROCEDURES FILLING THE CLEANING AGENT CONTAINER Check that: VENT The vent port is above the PORT connector and pointing toward you. CONNECTOR The tubing is not kinked or twisted. 7027109C Close the reagent drawer. Do you want to analyze samples? Press the RUN You are done.

  • Page 48: Emptying The Waste Container

    REPLACE/ADJUST PROCEDURES EMPTYING THE WASTE CONTAINER EMPTYING THE WASTE CONTAINER Your instrument has a 4-L waste container that sits in a bracket on the right side of the Power Supply. Empty it and follow these steps to put chlorine bleach in it. ®...
  • Page 49 REPLACE/ADJUST PROCEDURES EMPTYING THE WASTE CONTAINER WARNING To prevent injury, avoid skin contact with the waste container and its associated tubing. The waste container and its associated tubing might contain residual biological material and must be handled with care. Clean up spills immediately. Dispose of the contents of the waste container in accordance with your local regulations and acceptable laboratory procedures Unscrew the cap and lay it on a leakproof disposable container,...
  • Page 50 REPLACE/ADJUST PROCEDURES EMPTYING THE WASTE CONTAINER Screw the cap back on. 7027053C Wipe the bracket with a paper towel moistened with a 10% bleach solution, and then return the container to the bracket. Do you want to analyze samples? Press the RUN You are done.

  • Page 51: Replacing The Sheath Filter

    REPLACE/ADJUST PROCEDURES REPLACING THE SHEATH FILTER REPLACING THE SHEATH FILTER The 0.2-µm sheath filter is in the Cytometer reagent drawer. Replace the sheath filter, Every 6 months. Whenever the sample flow rate is too high (repeated data rate warnings or system pressure warnings).
  • Page 52 REPLACE/ADJUST PROCEDURES REPLACING THE SHEATH FILTER CAUTION Damage to the instrument can occur if you do not install the sheath filter correctly. It allows fluid to flow in one direction only. Make sure you install the new sheath filter correctly. Pick up the old sheath filter, and notice how the three tubes are SHEATH...

  • Page 53: Replacing The Manual Sample Head

    REPLACE/ADJUST PROCEDURES REPLACING THE MANUAL SAMPLE HEAD Are bubbles in the vent tubing? Go to Close the reagent Heading 2.4, drawer, and press Venting Air the RUN button. Bubbles The indicator in the button glows (not glashes) green. Do the quality control procedure in the Startup chapter of the Operator’...
  • Page 54 REPLACE/ADJUST PROCEDURES REPLACING THE MANUAL SAMPLE HEAD At the sample stage, remove the two Allen screws securing the test tube guide around the sample head. Remove the test tube guide and set it aside. 7027118C Pull down on the sample head while turning it to the left and slide it off the sample probe.
  • Page 55 REPLACE/ADJUST PROCEDURES REPLACING THE MANUAL SAMPLE HEAD Slide the new sample head onto the sample probe and up into the head seat with the tubing facing the back. SAMPLE PROBE SAMPLE HEAD 7027120C Install the test tube guide using the two Allen screws from step 2.

  • Page 56: Adjusting The System Pressure

    REPLACE/ADJUST PROCEDURES ADJUSTING THE SYSTEM PRESSURE ADJUSTING THE SYSTEM PRESSURE The startup procedure in the Operator's Guide tells you to check the System PRESSure gauge reading on the Power Supply daily. If the reading is not 30 ±2 psi, follow these steps to adjust it. FILTER WATER TRAP...
  • Page 57 REPLACE/ADJUST PROCEDURES ADJUSTING THE SYSTEM PRESSURE Push in on the collar to lock it into place. 7297134A Record that the system pressure was adjusted on the QC Mainenance screen. See Appendix B for instructions. 2-19 PN 4237296CA...

  • Page 58: 2.10 Positioning The Neutral Density Filter

    REPLACE/ADJUST PROCEDURES POSITIONING THE NEUTRAL DENSITY FILTER 2.10 POSITIONING THE NEUTRAL DENSITY FILTER If your laboratory primarily analyzes large particles (for example, plant cells), use the neutral density (ND1) filter. You do not need to install it. The filter is already in the Cytometer.
  • Page 59 REPLACE/ADJUST PROCEDURES POSITIONING THE NEUTRAL DENSITY FILTER Reposition the Cytometer center front panel. It snaps into place. If you decide to stop using the ND1 filter, pull out on the knob to unlock it, and then guide the knob to the right end of its track. 2-21 PN 4237296CA...

  • Page 60: 2.11 Resetting The Circuit Breakers

    REPLACE/ADJUST PROCEDURES RESETTING THE CIRCUIT BREAKERS 2.11 RESETTING THE CIRCUIT BREAKERS Check the indicators on the front of the Power Supply. If any of them are off, follow these steps to reset the appropriate circuit breakers: On the back of the Power Supply, find the circuit breaker that corresponds to the dark indicator on the front:...

  • Page 61: Troubleshooting

    TROUBLESHOOTING LEVEL SENSE INDICATORS Cleanse Low When the cleanse low indicator is red, fill the cleanse container. You cannot start a cleanse cycle until this is done. Sheath Low During sample analysis, you have 5 minutes to finish analyzing the current sample after the indicator starts flashing red.

  • Page 62: Error Message Log

    TROUBLESHOOTING ERROR MESSAGE LOG ERROR MESSAGE LOG The last 200 error messages from the Cytometer/instrument are stored in the error message log. Press Þ + z to access the error message log when you are in Acquisition or Listmode. ä å...

  • Page 63: Error Messages

    TROUBLESHOOTING ERROR MESSAGES ERROR MESSAGES Error messages appear: In the center of the Workstation screen, and In the lower right corner of the Acquisition Run screen (instrument errors only). In the error log, which appears after you press Þ + z. Refer to heading 3.2, Error Message Log, for details.
  • Page 64 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action An internal error has occurred Internal error occurred while 1. Exit SYSTEM II Software. initializing the Single or Dual Overlay 2. Restart SYSTEM II Software. screen. 3. From the Multigraph application, select the desired overlay screen.
  • Page 65 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Cannot get Cytometer ready Laser defective or not ignited. See Heading 2.11, Resetting the Circuit Breakers. No vacuum. Incorrect pressure. Cannot get sample information from Software error. Clean the carousel or use a new one. Bad bar-code label on carousel or sample tube.
  • Page 66 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Communication error Communication error between client 1. Exit program. and server. 2. Restart program. 3. Ensure server is running correctly. Confirmation failed When changing a supervisor password Make sure the new password is typed in the Maintenance screen, the user in the same way both times.
  • Page 67 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Cytometer read cyto state timeout Cytometer communication error. At the Workstation turn the system off, and then turn it back on. Data Memory Problem Memory failure. Reset computer. If error persists, call your Coulter Representative.
  • Page 68 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Equation(s) exceed defined limitations Software failure. At the Workstation turn the system off, and then turn it back on. ERROR: 2 parameter histograms not You selected a two-parameter Select a one-parameter histogram. allowed histogram as control or test.
  • Page 69 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Error in loading protocol Software cannot access protocol file. From File menu, select Rebuild. Error in rename listmode file File write error. Select Alt Save and repeat save. Hard disk error Error in sending all the compensation Workstation/Cytometer 1.
  • Page 70 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Error in writing file You specified the wrong path. Specify the correct directories on the Utilities Configuration screen. Full disk. Use archive on the Utilities Configuration screen and free up disk space.
  • Page 71 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action ERROR: Mouse Driver Not Present No mouse is detected. Tighten mouse connector. At the Workstation turn the system off, and then turn it back on. No mouse driver is installed. Call your Coulter Representative.
  • Page 72 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Error validating your entry An internal error occurred when 1. Exit SYSTEM II Software. creating a minimum counter. 2. Call your Coulter Representative. ERROR writing to file [file name]! Software cannot save information to Specify the correct directories on the Possible full disk...
  • Page 73 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action [Filename] does not exist in this Error opening the current panel file. 1. Exit SYSTEM II Software. directory! 2. Restart the program. 3. If error persists, reboot the Workstation. File name is <QC file name>...
  • Page 74 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Initializing Empty Template Program did not find a template for If this is a new template, just press Û to acknowledge the message. this panel. If trying to load an existing template, try rebuilding the directory.
  • Page 75 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Invalid Loadlist Panel size changed in The Panel size you want to continue Reload the Panel. [panel name] has been modified (protocols have been added or deleted). Invalid Loadlist Protocol changed in The Panel you want to continue has Reload the Panel.
  • Page 76 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action LAST ENTRY ERASED IN [file name] Last entry in QC file has been erased. None. Last entry in <QC file name> was After running an _A, _C or _Q type Erase the corresponding entry in the erased.
  • Page 77 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action MCL Door Open Error MCL door is open while the MCL is in Close MCL door. use. MCL Door Open Warning MCL EPROM Error MCL hardware error. Call your Coulter Representative. MCL Error MCL hardware error.
  • Page 78 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Missing Protocol(s). Panel is not Protocols are missing from Panel Ensure all protocols are present in the loaded. being loaded. panel/protocol directory. Must be greater than previous level [n] You entered a smaller scaling value Enter a larger scaling value.
  • Page 79 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action No parameter selected You tried to acquire data before Select parameters first. selecting any parameters. No reports queue has been selected. Queue has not been selected. Change directory or select a report. No template possible for Loadlist A patient report template cannot be Define a patient report template for the...
  • Page 80 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Not enough space for COPY There are insufficient data entry lines Ensure that the selected panel(s)’s on the Worklist to copy the selected protocols do not exceed the number of panel.
  • Page 81 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action [Panel name]: Invalid panel selected Occurs when a panel is selected and a Ensure that all protocols within the protocol is missing from that panel. panel are present in the selected [deleted protocol]: Protocol is deleted.
  • Page 82 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action PRINTER NOT READY! Printer is not on-line or is out of paper. Put Printer on-line or add paper as needed. Check Printer for error message. At the Workstation turn the system off, and then turn it back on.
  • Page 83 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Read Cyto State timeout The Workstation timed out while Repeat operation. If error persists waiting for the Cytometer to send state power OFF the Cytometer then restart information. the SYSTEM II Software program. Rectilinear regions not allowed in The system cannot assign a rectilinear Change region type and re-create...
  • Page 84 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Restarting MCL because: MCL/Panel The MCL could not continue because The only choice is to reload the panel panel name mismatch at entry the panel name corresponding to the or run the panel from the beginning [carousel entry num] protocol at the given entry in the...
  • Page 85 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Seek error [record #] or [offset #] Damaged QC file. Delete QC file and allow program to recreate it. The record number located at the Rerun the protocol. Run the panel if given offset could not be obtained.
  • Page 86 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action SHEATH LEVEL LOW! Add fluid. When Low sheath fluid or sheath sensor Fill sheath container. cyto is READY, press Run failed. If the sheath container is full but the error still occurs, call your Coulter Representative.
  • Page 87 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Specimen ID change NOT allowed Editing of Spec ID not allowed if call Do not modify the specimen ID. came from patient report pop-up. State Machine Failure Cytometer software failure. Record an entry in the instrument log.
  • Page 88 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action System Vacuum Okay The vacuum supply was outside the None. instrument’s operating range but is okay now. TARGET HISTOGRAM INCOMPATIBLE When attempting to copy a region, the Ensure that the number of parameters destination histogram is not in the destination histogram match the compatible with the source histogram...
  • Page 89 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action To cont. MCL wait for msg After sheath low or waste full, this Follow instructions on message message appears. -Insert Sample Tube-, press AUTO To use ‘Save’ a protocol must be An attempt was made to save a QC Select a protocol by activating Select loaded...
  • Page 90 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Tube ID does not match barcode. MCL While running a Worklist using the Inspect the MCL and make sure that will be stopped. MCL the tube ID assigned to the each tube barcode matches its current protocol did not match the corresponding tube ID in the Worklist.
  • Page 91 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action UNABLE TO COPY - MAXIMUM When copying an amorphous region, Try deleting some amorphous regions NUMBER OF AMORPHOUS REGIONS the system is unable to complete the before assigning new ones. task since the protocol already has the maximum number of eight amorphous regions allowed.
  • Page 92 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Unable to load hematology info from Loading hematology information from Check Hematology ID in database is database database failed. same as Specimen ID. UNABLE TO LOAD LISTMODE FILE Wrong types of data file, or corrupted Select another Listmode file.
  • Page 93 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Unable to open template file File load error. Specify the correct directory on the Utilities Configuration screen. From File menu, select Rebuild. Unable to print with no histograms This message is displayed when there Select histogram to print, if none are no histograms for printing.
  • Page 94 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Unable to save protocol file Current You specified wrong path. Specify the correct directories on the operation aborted Utilities Configuration screen. Disk full. Use archive on the Utilities Configuration screen and free up disk space.
  • Page 95 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action VDI display device driver not loaded No VDI graphics are loaded. Put the software program diskette in properly. drive A. At the A:\ prompt, type install. Warning: Less than 4 megabytes of Less than 4 MB is free on the hard Use archive on the Utilities disk space available...
  • Page 96 TROUBLESHOOTING ERROR MESSAGES Table 3.1 Error Messages (Continued) Message Cause Action Wrong Date format or out of range When entering the date, either an Enter correct format and make sure illegal date was entered or the format the day does not exceed max month was not as DDMMMYY (for example, day.
  • Page 97 TROUBLESHOOTING ERROR MESSAGES 3-37 PN 4237296CA...
  • Page 98 TROUBLESHOOTING ERROR MESSAGES 3-38 PN 4237296CA...
  • Page 99 TROUBLESHOOTING ERROR MESSAGES 3-39 PN 4237296CA...
  • Page 100 TROUBLESHOOTING ERROR MESSAGES 3-40 PN 4237296CA...

  • Page 101: Bar-Code Options

    BAR-CODE OPTIONS BAR-CODE READER An optional hand held bar-code reader is available for your system. Refer to the bar-code reader manufacturer’ s manual for specific instructions about using it. The illustration to the right shows how to connect the bar-code reader to your system.

  • Page 102: Bar-Code Printer Switch Settings

    BAR-CODE OPTIONS BAR-CODE PRINTER Bar-Code Printer Switch Settings The illustration to the right shows how to set the bar-code printer switch settings for use with your system. 7296008A PN 4237296CA...

  • Page 103: Qc Maintenance Screen

    QC MAINTENANCE SCREEN WORKING WITH THE QC MAINTENANCE SCREEN This screen maintains a dated maintenance log for your records, including the operator ID symbol for each of the listed actions. Use the QC Maintenance screen to record: Daily start up actions Daily shutdown actions Cleaning procedures Replacement procedures...

  • Page 104: Printing The Screen

    QC MAINTENANCE SCREEN EDITING THE MAINTENANCE SCREEN (OPERATOR LEVEL) Printing the Screen If you want to print the screen for your laboratory records: File tt Print EDITING THE MAINTENANCE SCREEN (OPERATOR LEVEL) Assigning An Operator ID To A Colored Box You need to assign your operator ID to one of the colored boxes at the right of the screen before you can edit the QC Maintenance screen.

  • Page 105: Making An Entry

    QC MAINTENANCE SCREEN EDITING THE MAINTENANCE SCREEN (OPERATOR LEVEL) Making an Entry Click on the colored box with your operator ID in it. 7296014B Click on the square for each action completed today. Your color and symbol appear in each selected square. Note: Only a supervisor can make entries on prior days.

  • Page 106: Editing The Maintenance Screen (Supervisor Level

    QC MAINTENANCE SCREEN EDITING THE MAINTENANCE SCREEN (SUPERVISOR LEVEL) EDITING THE MAINTENANCE SCREEN (SUPERVISOR LEVEL) Only a supervisor can: Make an entry on a day prior to today. Erase entries made on a day prior to today. Making Or Deleting An Entry Click on the black SUPVR box.

  • Page 107: Level),

    QC MAINTENANCE SCREEN CHANGING THE PASSWORD (SUPERVISOR LEVEL) CHANGING THE PASSWORD (SUPERVISOR LEVEL) Click on the SUPVR box with the right mouse button. 7296013B Type in your OLD (current) password at the system prompt and press Û. When doing this for the first time 7296016B after system installation there is no OLD password.

  • Page 108: Level),

    QC MAINTENANCE SCREEN CHANGING THE PASSWORD (SUPERVISOR LEVEL) PN 4237296CA...
  • Page 109 INDEX Symbols %Lymph + %Mono +%Gran + %Blast > 100.0, Calibrator Region already exists, Cannot assign CALIBRATOR to a Quadstat, Cannot find this region, Cannot get Cytometer ready, A region must first be selected, Cannot get sample information from MCL, adaptor, cleaning Cannot open protocol queue file, how to use, 1-24,...
  • Page 110 INDEX Connections to database have been disabled, due to error., 3-26 container, reagent Duplicate entry not possible, replacing, 1-11 container, waste replacing, 2-10 emptying waste container, 2-10 containers, replacing Equation(s) exceed defined limitations, reagent, 1-11 err Reading | Writing, conventions ERROR used in the manual, 2 parameter histograms not allowed,...
  • Page 111 INDEX state, Unable to access file, 3-30 Error in writing file, 3-10 UNABLE TO PROCESS, 3-33 Error in writing file, Current operation File name is, 3-13 aborted, 3-10 File problem, 3-13 Error initializing protocol, 3-10 File Write Error -, 3-13 Error installing clock interrupt vector, 3-10 , 3-12,...
  • Page 112 INDEX (A to Z) and (0 - 9), 3-15 MCL CPU error, 3-16 Invalid path selected, 3-15 MCL Door Open Error, 3-17 Invalid region id, 3-15 MCL Door Open Warning, 3-17 Invalid region index. Call for service., 3-15 MCL EPROM Error, 3-17 Invalid user id or password, 3-15...
  • Page 113 INDEX Invalid value for column, 3-15 bar-code printer, how to connect, Run time SQL error --, 3-24 bar-code printer, switch settings, to a, bar-code scanner/reader, how to connect, Name must not consist entirely of spaces. Out of MEMORY printing cytosettings, 3-20 DEFAULT is reserved, 3-18...
  • Page 114 INDEX adjustment. See adjustment procedures, record on Maintenance screen, cleaning. See cleaning procedures, sample head, 2-15 replacement. See replacement procedures, sheath filter, 2-13 Failure to resume Protocol sheath fluid, 3-12 Replay Not Active, 3-23 Unable to access file, 3-30 Replaying Data For Listmode Files Saved, 3-23 Protocol not valid, 3-22...
  • Page 115 INDEX cleaning, fillling, TARGET HISTOGRAM INCOMPATIBLE, 3-28 Sheath Drawer Error, 3-25 TARGET HISTOGRAM MUST BE Sheath Drawer Okay, 3-25 DIFFERENT, 3-28 Sheath Drawer Warning, 3-25 Temperature Error, 3-28 sheath filter Temperature Okay, 3-28 replacing, 2-13 Temperature Sensor Failure, 3-28 sheath fluid Temperature Warning, 3-28 container, cleaning,...
  • Page 116 INDEX Unable to assign histogram memory, 3-30 state, 3-34 Unable to autogate, 3-30 Unable to start database engine, 3-34 Unable to change histogram display, 3-30 Unable to start specified database, 3-34 Unable to create the histogram queue, 3-31 Unable to write PCX file, 3-34 Unable to create the regions display, 3-31...
  • Page 117 INDEX Worklist file not found, 3-35 Worklist type panel may not be edited, 3-35 Wrong Date format or out of range, 3-36 Wrong file, 3-36 Wrong password, 3-36 Wrong Time format or out of range, 3-36 X and y channel count exceeded 65535, 3-36 X channel count exceeded 65535, 3-36...
  • Page 118 INDEX INDEX-10 PN 4237296CA...
  • Page 119 TRADEMARKS The Beckman Coulter logo, COULTER, COULTER CLENZ, CYTO-TROL, EPICS, Immuno-Trol, IsoFlow, SYSTEM II, XL, and XL-MCL are trademarks of Beckman Coulter, Inc. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.
  • Page 120 PN 4237299 Master Index Combined index for the Operator's Guide, Special Procedures and PN 4237295 Troubleshooting, Reference, Getting Started, and Data Management manuals. Come visit us at www.beckmancoulter.com Printed on Recycled Paper Copyright © Beckman Coulter, Inc. 1996-2010 All Rights Reserved...

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