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Summary of Contents for Beckman Coulter J-Lite JLA-12.500
- Page 1 Instructions For Use JLA-12.500 Fixed-Angle Rotor Assembly For Use in Beckman Coulter Avanti JXN Series Centrifuges Only C70011AA April 2021 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
- Page 2 PN C70011 (April 2021) © 2021 Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
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Page 3: Revision Status
Revision Status For labeling updates, go to www.beckman.com/techdocs and download the latest version of the manual or system help for your instrument. Initial Issue C70011AA, 04/21 C70011AA... - Page 4 Revision Status C70011AA...
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Page 5: Safety Notice
Beckman Coulter, Inc. urges its customers and employees to comply with all national health and safety standards such as the use of barrier protection. This may include, but is not limited to, protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this or any other laboratory centrifuge. - Page 6 Although rotor components and accessories made by other manufacturers may fit in the JLA-12.500 rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the JLA-12.500 rotor, excluding the tubes and...
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Page 7: Safety During Installation And/Or Maintenance
Beckman Coulter offers the Field Rotor Inspection Program (FRIP). This program involves a visit to your laboratory by a specially trained Beckman Coulter representative, who will inspect all your rotors for corrosion or damage. -
Page 8: Chemical And Biological Safety
Refer to applicable external device user’s manuals for operating procedures of that device. NOTE For Safety Data Sheets (SDS/MSDS) information, go to the Beckman Coulter website at https://www.beckman.com/techdocs. WARNING Risk of chemical injury from bleach. To avoid contact with the bleach, use barrier protection, including protective eyewear, gloves, and suitable laboratory attire. - Page 9 Safety Notice Chemical and Biological Safety procedures. • Operate the instrument in accordance with the instructions outlined in this manual and take all the necessary precautions when using pathological, toxic, or radioactive materials. • Splashing of liquids may occur; therefore, take appropriate safety precautions, such as using safety glasses and wearing protective clothing, when working with potentially hazardous liquids.
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Page 10: Mechanical Safety
Beckman Coulter offers the Field Rotor Inspection Program (FRIP). This program involves a visit to your laboratory by a specially trained Beckman Coulter representative, who will inspect all of your rotors for corrosion or damage. -
Page 11: Disposal
Safety Notice Disposal Disposal Clean and decontaminate the rotor per the Care and Maintenance section of this manual before disposal. Users are encouraged to check with local waste disposal authorities for specific disposal requirements. C70011AA... - Page 12 Safety Notice Disposal C70011AA...
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Page 13: Table Of Contents
Contents Revision Status, iii Safety Notice, v Alerts for Warning, Caution, Important, and Note, v Safety Information for the J-Lite JLA-12.500 Fixed-Angle Rotor Assemblies, vi Safety During Installation and/or Maintenance, vii Radiation Safety, vii Chemical and Biological Safety, viii Mechanical... - Page 14 Contents Replacement Rotor Parts, 20 Supplies, 20 Beckman Coulter, Inc. J Series Rotor Warranty...
- Page 15 Illustrations Illustrations Dual Locking Rotor Lid, 2 The JLA-12.500 Fixed Angle Rotor, 3 Arranging Loads in the Rotor, 5 Assembly, 5 Rotor Hub Teeth Location and Orientation, 6 Rotor Top View: Indicator Marks Show Rotor Ribs’ Orientation, 6 Tie-down Knob and Daisy Knob, 7 Check for Corrosion and Cracks, 15...
- Page 16 Rotor Assembly Use only the items listed here and observe fill volumes and maximum run speeds ., 10 Beckman Coulter Adapters for Commercially Available Tubes for Use in the JLA-12-500 Rotor, 12 Relative Centrifugal Fields for the JLA-12.500 Rotor Assembly, 13...
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Page 17: J-Lite Jla-12.500
J-Lite JLA-12.500 Fixed-Angle Rotor Assembly Specifications Maximum speed ..........12,000 RPM Critical speed range . -
Page 18: Description
The JLA-12.500 fixed angle rotor, rated for 12,000 rpm, is designed to centrifuge up to six 500 mL containers at a 30-degree angle to the axis of rotation. Used in Beckman Coulter Avanti JXN-26 and JXN-30 series centrifuges (not for use in older Avanti J-Series), the rotor develops centrifugal forces that can efficiently pellet cells from large volumes of culture media, cell particles from tissue homogenates, or subcellular organelles. - Page 19 The JLA-12.500 rotor with dual-locking lid has been tested to demonstrate containment of microbiological aerosols under normal operating conditions of the associated Beckman Coulter centrifuge, when used and maintained as instructed. Ensure the O-ring is properly installed and lubricated. The dual-locking capability provides added biosafety by allowing the rotor to be loaded into and removed from the centrifuge with the lid in place.
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Page 20: Preparation And Use
Although rotor components and accessories made by other manufacturers may fit in the JLA-12.500 rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the JLA-12.500 rotor, excluding the tubes referenced in... -
Page 21: Operation
J-Lite JLA-12.500 Fixed-Angle Rotor Assembly Preparation and Use Figure 3 Arranging Loads in the Rotor Two, three, four, or six loads can be centrifuged per run, if the bottles are placed in the cell holes in the rotor as shown. -
Page 22: Installing The Rotor
J-Lite JLA-12.500 Fixed-Angle Rotor Assembly Preparation and Use b. Run Speeds for information about speed limitations. Installing the Rotor Two metal ribs inside the rotor drive hole engage with pins on the centrifuge drive hub to prevent the rotor from slipping during acceleration and deceleration... - Page 23 J-Lite JLA-12.500 Fixed-Angle Rotor Assembly Preparation and Use Turn the rotor to properly align the markings to ensure the ribs in the rotor body are properly aligned with the drive hub teeth. Once the rotor is properly aligned carefully lower it straight down onto the hub. When the rotor is properly installed, the ribs fit on the drive hub teeth.
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Page 24: Run Procedure
J-Lite JLA-12.500 Fixed-Angle Rotor Assembly Preparation and Use Secure the rotor to the centrifuge drive hub by pressing the round tie-down knob (Figure down and turn it to the right (clockwise) until secure. Do not overtighten. WARNING Securely tighten the round tie-down knob to attach the rotor body to the centrifuge drive hub. -
Page 25: Removal And Sample Recovery
J-Lite JLA-12.500 Fixed-Angle Rotor Assembly Preparation and Use Removal and Sample Recovery WARNING Risk of personal injury. To avoid injury due to moving parts, observe the following: • Never attempt to exchange labware or reagents while the instrument is operating. -
Page 26: Tubes And Bottles
Resistances for Beckman Coulter Centrifugation Products (IN-175). Beckman Coulter does not recommend the use of one tube in preference to another, nor guarantee the acceptability of the sample tube to produce quality results. If you need information on a tube... - Page 27 These speeds have been achieved in testing at Beckman Coulter, but, because of variations in materials and chemicals the product comes in contact with, no guarantee of performance or fit is expressed or implied.
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Page 28: Run Times
J-Lite JLA-12.500 Fixed-Angle Rotor Assembly Run Times Table 2 Beckman Coulter Adapters for Commercially Available Tubes for Use in the JLA-12-500 Rotor Tube Required Accessory Number Max Fill of Tubes Dimensions Vol. Part and Volume Description (mL) Description Number adapter... -
Page 29: Run Speeds
J-Lite JLA-12.500 Fixed-Angle Rotor Assembly Run Speeds operating at the bottle’s maximum speed is 1277. Substituting k rated into equation (2) produces a k factor which can then be used in equation (1) to estimate run times. For more information on k factors, search Beckman.com... - Page 30 J-Lite JLA-12.500 Fixed-Angle Rotor Assembly Run Speeds Table 3 Relative Centrifugal Fields for the JLA-12.500 Rotor Assembly (Continued) Relative Centrifugal Field (x g) At r At r At r Rotor Speed (rpm) (166.7 mm) (105.1 mm) (43.5 mm) 4,000 2,990...
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Page 31: Care And Maintenance
J-Lite JLA-12.500 Fixed-Angle Rotor Assembly Care and Maintenance Care and Maintenance Maintenance Figure 8 Check for Corrosion and Cracks CAUTION Risk of equipment damage. Corrosion begins in scratches and may open fissures in the rotor with continued use. Do not use sharp tools on the rotor that could cause scratches in the rotor surface. -
Page 32: Cleaning
J-Lite JLA-12.500 Fixed-Angle Rotor Assembly Care and Maintenance Regularly lubricate the rotor drive’s hole and the metal threads of the rotor body, daisy knob, and tie-down knob with a thin, even coat of Spinkote lubricant. Failure to keep these threads lubricated can result in damaged threads or sticking knobs. -
Page 33: Decontamination
Decontamination If the rotor body becomes contaminated with radioactive material, it should be decontaminated using a solution that will not damage the anodized surfaces. Beckman Coulter has tested Radiacwash and found that it does not harm anodized aluminum. -
Page 34: Sterilization And Disinfection
Only hydrogen peroxide solution is recommended. Use of Vaporous Hydrogen Peroxide will cause permanent damage to anodized surfaces. While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use. -
Page 35: Storage
Returning a Rotor Before returning a rotor or accessory for any reason, prior permission must be obtained from Beckman Coulter, Inc. A return authorization form may be obtained from your local Beckman Coulter sales office. The form should contain the following information: •... -
Page 36: Replacement Rotor Parts
C55767 Lid Assembly C72536 Lid O-ring Kit -- 961697 (large) and 961240 (small) C70015 Supplies NOTE For SDS/MSDS information, go to the Beckman Coulter Life Sciences website at www.beckman.com/techdocs. Description Part Number Bottles, tubes, and adapters Table 1 Table Bottle caps (for 500 mL bottles 361690 and 361691) - Page 37 A defective rotor will be replaced by Beckman Coulter at its then current list price less a credit based upon the age of the rotor (years since date of purchase). The Buyer shall not receive credit until the claimed defective rotor is returned to Beckman Coulter's Indianapolis, Indiana facility or delivered to a Beckman Coulter Field Service representative.
- Page 38 Beckman Coulter, Inc. J Series Rotor Warranty Warranty-2 C70011AA...
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Page 40: Related Documents
Using Tubes, Bottles, and Accessories • Using Fixed-Angle Rotors • Using Swinging-Bucket Rotors • Using Vertical-Tube and Rack-Type Rotors • Care and Maintenance • Chemical Resistances for Beckman Coulter Centrifugation Products • Temperature Compensation Tables • Gradient Materials • Blood Component Separation • References •...
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