Beckman Coulter ProteomeLab XL-A Instructions For Use Manual
  1. Manuals
  2. Brands
  3. Beckman Coulter Manuals
  4. Laboratory Equipment
  5. ProteomeLab XL-A
  6. Instructions for use manual
Beckman Coulter ProteomeLab XL-A Instructions For Use Manual

Beckman Coulter ProteomeLab XL-A Instructions For Use Manual

Protein characterization system
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
PN LXLAI-IM-10AB
February 2014
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
Instructions For Use
ProteomeLab XL-A/XL-I
Protein Characterization System

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the ProteomeLab XL-A and is the answer not in the manual?

Questions and answers

Related Manuals for Beckman Coulter ProteomeLab XL-A

Summary of Contents for Beckman Coulter ProteomeLab XL-A

  • Page 1 Instructions For Use ProteomeLab XL-A/XL-I Protein Characterization System PN LXLAI-IM-10AB February 2014 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
  • Page 2 Beckman Coulter, Inc. Beckman Coulter and the stylized logo are trademarks of Beckman Coulter, Inc. and are registered in the USPTO. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.
  • Page 3 Safety Notice Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative.
  • Page 4 Mechanical Safety For safe operation of the equipment, observe the following: • Use only the Beckman Coulter rotors and accessories designed for use in this instrument. • Do not exceed the maximum rated speed of the rotor or centerpiece(s) in use.
  • Page 5 • Dispose of all waste solutions according to appropriate environmental health and safety guidelines. It is your responsibility to decontaminate the instrument and accessories before requesting service by Beckman Coulter Field Service. PN LXLAI-IM-10AB...
  • Page 6 Safety Notice Location of Laser Caution Labels on the ProteomeLab XL-I Instrument Location of Laser Caution Labels on the ProteomeLab XL-I Instrument A laser caution label and a laser aperture caution label are affixed to the XL-I instrument as shown below. Laser Caution Label Name Rating Plate Back of Instrument...

  • Page 7: Table Of Contents

    Contents Safety Notice, iii Alerts for Warning, Caution, Important, and Note, iii Safety During Installation and/or Maintenance, iii Safety, iii Electrical Safety, iv Safety Against Risk of Fire, iv Mechanical Safety, iv Chemical and Biological Safety, v Location of Laser Caution Labels on the ProteomeLab XL-I Instrument, vi Introduction, xv Certification, xv...
  • Page 8 Contents Safety Features, 2-1 Door, 2-1 Barrier Ring, 2-2 Overspeed System, 2-2 Rotor Energy System, 2-2 Rotor Chamber, 2-2 Monochromator, 2-3 Optical Systems , 2-3 Absorbance, 2-5 Interference, 2-7 Vacuum System, 2-9 Temperature Sensing and Control, 2-10 Drive, 2-11 Rotor and Cells, 2-11 System Control, 2-11...
  • Page 9 Contents File Naming and Storage, 3-11 Scan SETTINGS Files, 3-12 Scan Data Files , 3-12 Files, 3-14 Data storage , 3-14 README FILE, 3-15 CHAPTER 4: System Operation, 4-1 Introduction, 4-1 Preparing the Rotor and Analytical Cells, 4-1 Starting the XL-A/XL-I User Interface Software, 4-2 Wavelength ScanS (Absorbance Optical System only), 4-4...
  • Page 10 Software, A-3 Adjusting the Screen Resolution, A-5 Uninstalling the XL-A/XL-I User Interface Software, A-6 APPENDIX B: Interference Theory, B-1 Description of Interference Theory, B-1 References, B-4 Glossary Beckman Coulter, Inc. ProteomeLab XL-A and XL-I Protein Characterization Systems Warranty Related Documents...
  • Page 11 Illustrations Illustrations The Rotor Chamber, 2-3 The XL-A Monochromator, 2-4 The XL-I Monochromator, 2-4 The Scanning UV/VIS Absorbance Optical System, 2-6 Top View of Reference and Sample Sectors in a Two-Channel Cell, 2-7 Monochromator Filter Lever Positions, 2-7 The XL-I Interference Optical System, 2-8 The Fringe Display, 2-9...
  • Page 12 Illustrations ProteomeLab XL-A or XL-I Electrical Requirements, A-2 ProteomeLab XL-A or XL-I Instrument and Data System Space Requirements, A-3 Wave Interference, B-1 Thomas Young’s Original Drawing of a Two-Source Interference Pattern, B-2 Interference Pattern Created by Two Slits, B-2 Light from O passes Through Slits M and N and Produces...
  • Page 13 Tables Tables PC System Controller Configuration, 1-4 Diagnostic Messages Appearing on the PC Screen, 5-5 Diagnostic Troubleshooting Chart, 5-9 Required Wire Connections, A-2 xiii...
  • Page 14 Tables...

  • Page 15: Safety Notice

    ISO 9001:2008 and ISO 13485:2003. They have been designed and tested to be compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable regulatory agencies. Declarations of conformity and certificates of compliance are available at www.beckmancoulter.com.

  • Page 16: Conventions

    Further, the use of any equipment other than that intended for use by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual is the sole responsibility of the user.

  • Page 17: Related Information On The Internet

    For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.
  • Page 18 Introduction Printer Notice xviii PN LXLAI-IM-10AB...

  • Page 19: Specifications

    CHAPTER 1 Specifications Specifications Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. Physical Data Description Specification Weight • Instrument only — 465 kg (1025 lb) • IBM computer — approx 9.5 kg (21 lb) •...

  • Page 20: Control Features

    500 rpm, no brake, maximum deceleration from set speed to 0 rpm Key switch used to select normal or locked operation; zonal operation not allowed Instrument classification T: uses Beckman Coulter analytical rotors only; preparative and zonal operation not allowed PN LXLAI-IM-10AB...

  • Page 21: Analytical Specifications

    5 seconds Interferometer precision approx  0.003 fringe PC System Controller Table 1.1 shows the configuration of the IBM PC system controller that Beckman Coulter has specified for use with the ProteomeLab XL-A or XL-I system. PN LXLAI-IM-10AB...
  • Page 22 Origin Technical Graphics and Data Analysis in Version 6.0 Windows NOTE Beckman Coulter is not responsible for system integration of computers that do not match the specifications listed in Table 1.1. Every effort has been made to eliminate operational problems from the ProteomeLab XL-A/XL-I user interface software.

  • Page 23: Hardware Description And Preparation

    Safety Features ProteomeLab XL-A and XL-I instruments have been designed and tested to operate safely indoors at altitudes up to 2000 m (6 562 ft). Safety features include the following.

  • Page 24: Barrier Ring

    Hardware Description and Preparation Rotor Chamber Barrier Ring A 41-mm (1.63-in.) steel alloy armor ring acts as the primary barrier, surrounded by a 12-mm (0.5-in.) vacuum chamber to provide full protection for the operator. Overspeed System The overspeed system is a safety feature designed to ensure that the rotor does not exceed its maximum speed.

  • Page 25: Monochromator

    Hardware Description and Preparation Monochromator Figure 2.1 The Rotor Chamber Hall Effect Sensor Safety Plate Drive Spindle Absorbance Slit Assembly Door Handle Photoelectric Device Radiometer Condenser Lens Monochromator Mounting Receptacle Monochromator In XL-A systems, the monochromator (see Figure 2.2) contains all components of the scanning UV/VIS absorbance optical system.

  • Page 26: The Xl-A

    Hardware Description and Preparation Optical Systems Figure 2.2 The XL-A Monochromator Photomultiplier Tube UV Light Source Figure 2.3 The XL-I Monochromator Laser Housing UV Light Path Housing PN LXLAI-IM-10AB...

  • Page 27: Absorbance

    Hardware Description and Preparation Optical Systems Absorbance The absorbance optical system (see Figure 2.4) is completely contained within the instrument chamber. The high-intensity xenon flash lamp provides light in the spectra from 190 to 800 nm. The light passes through the monochromator for wavelength selection. A toroidally curved holographic diffraction grating varies the wavelength and collimates the light.

  • Page 28: Cell

    Hardware Description and Preparation Optical Systems Figure 2.4 The Scanning UV/VIS Absorbance Optical System Toroidal Diffraction Grating Incident Light Detector Reflector Rotor Cell Sample/Reference Cell Assembly Movable Imaging System Slit (13 m) Aperture Xenon Flash Lamp Photomultiplier Tube PN LXLAI-IM-10AB...

  • Page 29: Interference

    Hardware Description and Preparation Optical Systems Figure 2.5 Top View of Reference and Sample Sectors in a Two-Channel Cell Sample Sector (contains sample Reference Sector and buffer) (contains buffer only) Keyway Figure 2.6 Monochromator Filter Lever Positions Monochromator Stem Parallel to stem Perpendicular (for scans below to stem (for...
  • Page 30 Hardware Description and Preparation Optical Systems Figure 2.7 The XL-I Interference Optical System Laser Diode Light Source Slits Condenser Lens Rotor Mirror Mirror Camera Camera Sensor Camera Lens Cylinder Lens Mirror Reflective Prism • Camera lens, which, together with the condenser lens, focuses the cell on the CCD sensor. •...

  • Page 31: Vacuum System

    Hardware Description and Preparation Vacuum System • Frame Grabber imaging hardware (installed in PC system controller) and real-time fringe display software (not shown), which work together to acquire the camera image of the cell and display it on the computer screen. •...

  • Page 32: Temperature Sensing And Control

    Hardware Description and Preparation Temperature Sensing and Control Figure 2.9 Plot of Interference Data Resulting from Fast Fourier Transformation Radius (cm) At the end of a run, the chamber vacuum must be vented (by pressing ) before the door can VACUUM be opened.

  • Page 33: Drive

    Rotor and Cells Two analytical rotors are available for use in ProteomeLab XL-A and XL-I systems: the An-60 Ti and the An-50 Ti. In the An-60 Ti, up to three cells and a counterbalance can be run at one time.

  • Page 34: Standby Mode

    Hardware Description and Preparation Instrument Controls A red POWER OFF button is located on the neck of the control head for your convenience (see Figure 2.10). Pressing the POWER OFF button trips the circuit breaker to the (off) position. To return power to the instrument, the circuit breaker must be returned to the up position.

  • Page 35: Hardkeys

    Hardware Description and Preparation Instrument Controls Figure 2.11 The Control Panel Video Parameter Activation Keypad Display Keys Keys PRINTER SPEED VACUUM TIME START TEMP Softkey STOP ROTOR ENTER Labels Parameter Keys Hardkeys NOTE Because analytical run settings are entered on the PC, the instrument control panel is not typically used (except for the key).

  • Page 36: Printer (If Equipped)

    Hardware Description and Preparation Pre-run Procedures Figure 2.12 Location of Adjustment Screw for Control of Video Display Brightness Adjustment Screw Printer (If Equipped) The printer, located within the control head, provides printouts of rotor and run data. A menu of printer options is accessed via the hardkey.

  • Page 37: Installing The Monochromator

    Hardware Description and Preparation Pre-run Procedures Assemble the rotor cells according to the instructions in the rotor manual. Precool the assembled rotor if required. Load the rotor into the chamber. Installing the Monochromator After the rotor is installed in the chamber, install the monochromator in the chamber as follows. Holding the monochromator at a 30-degree angle to the chamber bottom, align the index pins with the index pin holes and carefully insert the end with the red holding ring into the mounting receptacle (see...

  • Page 38: Activating Chamber Vacuum

    Hardware Description and Preparation Pre-run Procedures Activating Chamber Vacuum When a run begins, the rotor will not accelerate until the chamber pressure drops below 50 . If you activate the vacuum system prior to entering the scan settings, the chamber will be evacuated and the rotor can accelerate to set speed when you start the run.

  • Page 39: Chapter 3: Xl-A/Xl-I User Interface Software Description

    CHAPTER 4, System Operation. This section assumes that a Beckman Coulter Field Service representative has installed the software on your PC. Installation instructions are included in APPENDIX A, Installation Requirements in case you need to reinstall the software.

  • Page 40: Toolbar

    XL-A/XL-I User Interface Software Description Main Window The main window contains the following menus: • , for opening new and existing scan settings files, saving scan settings files, and exiting the File program; • for instrument setup and operation; • , for XL-A or XL-I scan settings file setup and operation;...

  • Page 41: File Menu

    XL-A/XL-I User Interface Software Description Main Window File Menu Choosing opens a new scan settings file window, shown below. New File The system automatically assigns a sequence number to the file name (NONAME01, NONAME02, etc.) to prevent files from being overwritten if they are not renamed before being saved Choosing opens an existing scan settings file.

  • Page 42: Xl Menu

    XL-A/XL-I User Interface Software Description Main Window XL MenU Choose to start the XL-A or XL-I using the settings Start XL entered in the XL Settings dialog box, shown below. Choosing brings the XL-A or XL-I to 0 rpm. Stop XL Choosing opens the XL Settings dialog box.

  • Page 43: Scan Menu

    XL-A/XL-I User Interface Software Description Main Window Choosing opens the following dialog box, which shows the current XL Monitor system status. This dialog box also opens automatically when a scan is started. The speed, time, and temperature settings are taken from the scan settings file, if a scan is running, or from the XL Settings dialog box if no scan is running.
  • Page 44 XL-A/XL-I User Interface Software Description Main Window command (used with absorbance scans only) allows you to view the data from the Request Data last scan performed, in absorbance, intensity, or incident format. During a scan, the system acquires scan data in all three formats and temporarily stores this data on the data acquisition board.
  • Page 45 XL-A/XL-I User Interface Software Description Main Window determining the real bottom of the cell, you can select a data subset which deletes all data points beyond 7.13 cm before importing the file for analysis. (See the XL-A/XL-I Data Analysis Software User’s Manual [LXL/A-TB-009] for instructions on selecting data subsets. A pdf file of this manual is located on the XL-A/XL-I Windows setup CD.) Cell bottom (approx.

  • Page 46: Interference Menu

    Shows the real-time laser status (checked is on, unchecked is off). Can also be checked or unchecked For Beckman Coulter manually to turn laser Field Service use on and of Click this button to initiate automat- ic laser delay adjustment (rather than using Laser delay slide bar) •...
  • Page 47 XL-A/XL-I User Interface Software Description Main Window knows the position of the other cell holes in relation to cell 3. One complete rotor rotation is 360 degrees. Cell hole 1 is opposite cell hole 3, at 180 degrees. Cell hole 2 is at 90 degrees, and cell hole 4 is at 270 degrees.

  • Page 48: Service Menu

    CAUTION Changing any of the service settings will affect system operation and could possibly result in system damage. Do not change settings accessed under the Service Menu except under the direction of a Beckman Coulter Field Service Engineer. Choosing displays the following submenus: Service >...

  • Page 49: Options Menu

    XL-A/XL-I User Interface Software Description File Naming and Storage Options Menu Choosing opens a dialog box where you can specify Options > Com Port which communication (com) port you want your PC to use for interfacing with the XL-A or XL-I. The default is com port 1. Keeping the default setting will help to avoid system problems.

  • Page 50: Scan Settings Files

    XL-A/XL-I User Interface Software Description File Naming and Storage Scan SETTINGS Files Scan settings files contain the user-specified run settings. New scan settings files are opened by selecting . After a scan settings file has been saved, reopen it by selecting File >...
  • Page 51 XL-A/XL-I User Interface Software Description File Naming and Storage Scan Data Each scan data file has a two-line header containing information about that file. The header appears at the top of the page when you open the file in a text or word processing program. An example absorbance data file, opened in Windows Notepad, is shown below.

  • Page 52: Log Files

    XL-A/XL-I User Interface Software Description File Naming and Storage Interference Data File Header Log Files Log files contain a record of all scans performed, scan settings used, and any diagnostics that occurred during a scan or method. The log files are saved to the same directory as the scan data. The system creates separate log files for interference and absorbance scans.

  • Page 53: Readme File

    XL-A/XL-I User Interface Software Description README FILE Organizing Your Files Use the Windows Explorer to view and organize the files on your computer. See the Windows instruction manual or online help for more details. README FILE The XL-A/XL-I Readme file includes a description of any last-minute information that could not be added to this manual before it went to press.
  • Page 54 XL-A/XL-I User Interface Software Description README FILE 3-16 PN LXLAI-IM-10AB...

  • Page 55: System Operation

    CHAPTER 4 System Operation Introduction This section describes ProteomeLab XL-A and XL-I operating procedures, including • preparing the analytical rotor and cells; • setting up and saving scan settings files; • recalling previously saved scan settings files; • displaying data;...

  • Page 56: Starting The Xl-A/Xl-I User Interface Software

    System Operation Starting the XL-A/XL-I User Interface Software b. Sedimentation equilibrium: using a six-channel centerpiece, place approximately 110 L total of sample plus solvent in each sample channel and 125 L of buffer in each buffer channel. Interference scans require less difference in volume; approximately 5 L difference is acceptable.
  • Page 57 System Operation Starting the XL-A/XL-I User Interface Software Choose File > New File Or, click the new file icon (shown to the left). A blank scan settings file opens (shown below). Scan settings files are assigned a sequence number (01, 02, Use these system settings Check this box to use etc.) to prevent overwriting if...

  • Page 58: Wavelength Scans (Absorbance Optical System Only)

    System Operation Wavelength ScanS (Absorbance Optical System only) Wavelength ScanS (Absorbance Optical System only) Description During a wavelength scan, different wavelengths of light (from 190 to 800 nm, user-selectable) are passed through specified radial positions across the cell. The resulting absorbance spectrum shows the absorbance value (...
  • Page 59 System Operation Wavelength ScanS (Absorbance Optical System only) Click the button. Detail The Wavelength Detail dialog box appears. Maximum radial position, Step is the only between user-entered mode available for minimum radius and wavelength scans 7.30 cm. Enter 0 to take Click the appropriate 1 scan at the radius button for the type of...
  • Page 60 System Operation Wavelength ScanS (Absorbance Optical System only) Click the box next to each option you wish to activate. • Do not use the option with wavelength scans. Filter scan intensities below 50 • Radial calibration should be done at low speeds (approximately 3000 rpm) for optimum accuracy.

  • Page 61: Setting Up Wavelength Methods

    System Operation Wavelength ScanS (Absorbance Optical System only) Choose OK to use the selected options; choose if you do not want to activate any of Cancel the options. The Scan Options dialog box closes. At this point you can either start the scan, or save the scan settings to a file. To start the scan, a.

  • Page 62: Sedimentation Velocity Scans

    System Operation Sedimentation Velocity Scans In the setup area for the cell to be used in the method, click the button. Wavelength button at the top left of the window becomes active. Method Click the button. Method A window appears in which multiple scans can be set up at different speeds and temperatures, with varying amounts of time between scans.

  • Page 63: Plot Of Suggested Rotor Speed Versus Molecular Weight For Two

    System Operation Sedimentation Velocity Scans Circled numbers correspond to numbered steps in the text If you want to use the same scan settings for each cell, enter settings for Cell 1 and then click box. All Settings Identical to Cell 1 Figure 4.2 can help you determine the run speed required for your experiment.
  • Page 64 System Operation Sedimentation Velocity Scans Click the button. Detail The Velocity Detail dialog box appears. Settings specific to the absorbance system are in the left half of the window. Optional fields for input of a second and third scan wavelength, from 190 to 800 nm Amount of radial movement per step, from 0.001 to 0.01 cm...

  • Page 65: Setting Up Sedimentation Velocity And Sedimentation Equilibrium Scans: Interference System

    System Operation Sedimentation Velocity Scans Setting Up Sedimentation Velocity and Sedimentation Equilibrium Scans: Interference System This section contains steps for setting up the interference optical system for equilibrium and velocity scans. The steps for setting up the laser are shown below, along with recommended settings for cell of 1 of a 4-hole rotor.
  • Page 66 Adjusting the Laser Settings settings to a scan settings file called “masterfile” (or the name of your choice. Your Beckman Coulter Field Service engineer may have set up such a file during installation.) Follow the steps below to create a master file.
  • Page 67 Interference > Laser Setup Adjust the laser settings as described above and click the button. Auto Adjust Laser Delay Alternately, input settings that you (or a Beckman Coulter Field Service engineer) have experimentally determined to be optimum. Click the button.
  • Page 68 System Operation Sedimentation Velocity Scans Fringe Display – Cell 4 Zoom Circled numbers correspond to numbered steps in the text Click the button. Inside Click once on the fringe display at the position representing the inner edge of the counterbalance, approximately 5.85 cm from the axis of rotation. This position is indicated by a black dot at the upper left of the figure above.
  • Page 69 System Operation Sedimentation Velocity Scans Click the button to accept this value. Set Radius Click to close the dialog box. Enter Scan Settings Open the master laser settings file by selecting , and then selecting the File > Open File appropriate master file name.
  • Page 70 System Operation Sedimentation Velocity Scans Start the Instrument Choose from the XL menu. Start XL Or click the green start icon in the toolbar. At 3000 rpm, fringes should appear in the fringe display when the laser is turned on. In the new scan settings file, in the setup area for the appropriate cell, click the box.
  • Page 71 System Operation Sedimentation Velocity Scans Check/Adjust the Pixels Per Fringe Setting Check the pixels per fringe setting. a. Click once on the fringe display anywhere to the right of the meniscus. b. Click the button. Automatic c. Repeat steps 7a and 7b at several radial locations to determine an average pixels per fringe for the whole image.
  • Page 72 System Operation Sedimentation Velocity Scans These settings determine the portion of the cell to be included in the scan. a. In the Radii setup area of the Velocity Detail window, click , then click the fringe Inside display once on the left edge of the area to be scanned, typically to the right of the meniscus.
  • Page 73 System Operation Sedimentation Velocity Scans To set up and use a new blank scan during a run, a. In the Velocity Detail window, click the check box; then click the Blank scan subtraction button. Blank Setup The Blank Setup window for the selected cell appears. b.
  • Page 74 System Operation Sedimentation Velocity Scans g. In the Velocity Detail window, click the box; then click the Blank scan subtraction Blank button. Setup h. In the Blank Setup window, check the box and click the button. Use blank scan file Browse The Select Blank File dialog box appears, where you can search for a previously saved blank file to use.
  • Page 75 System Operation Sedimentation Velocity Scans Select the blank file to use from the list of files. Navigate to the file if necessary. Click . The selected file appears in the Blank File Information field on the Blank Setup window. When you start the scan, the system opens the specified blank scan and automatically subtracts it.

  • Page 76: Setting Up Sedimentation Velocity Methods

    System Operation Sedimentation Velocity Scans Setting Up Sedimentation Velocity Methods Sedimentation velocity methods can contain up to 999 scans. You can specify a time to wait before beginning the scans, a time between scans, and the total number of scans. The speed, time, and temperature settings used for velocity methods appear in the XL Settings area at the top of the scan settings window.

  • Page 77: Sedimentation Equilibrium Scans

    System Operation Sedimentation Equilibrium Scans To run the method, click the button on the scan settings window. Start Method Scan NOTE To ensure consistent time delays between scans, do not use your computer for other tasks while a method is running. This is especially important with velocity scans, in which the scans must be taken at precisely timed intervals.
  • Page 78 System Operation Sedimentation Equilibrium Scans If you want to use the same scan settings for each cell, enter settings for Cell 1 and then click box. All Settings Identical to Cell 1 Enter the Rmin (minimum radius), Rmax (maximum radius) and W1 (wavelength) values in the appropriate boxes.
  • Page 79 System Operation Sedimentation Equilibrium Scans Saves the scan data in Cancels the automatic delay intensity format rather calibration before the first than absorbance, scan and after each speed which is the default change of 1000 rpm or more. Uses the previous calibration Discards noisy, low- value.
  • Page 80 System Operation Sedimentation Equilibrium Scans When scan is complete, a plot of the scan appears automatically. If leakage has occurred, indicated by movement of the meniscus, reload the cell(s) and repeat the scan. Check and adjust settings as needed. Speed and Time Settings for Equilibrium Scans Figure 4.3 shows suggested speed ranges for use when the molecular weight and approximate sedimentation coefficient are known.

  • Page 81: Setting Up Sedimentation Equilibrium Methods

    System Operation Sedimentation Equilibrium Scans Setting Up Sedimentation Equilibrium Methods You can include up to 9900 scans in equilibrium and wavelength methods. This section discusses equilibrium methods; however, follow the same steps when setting up wavelength methods. NOTE Although the maximum number of scans in a method is 9900, this number is actually limited by the size of your hard disk.

  • Page 82: Using Previously Saved Scan Settings

    System Operation Using Previously Saved Scan Settings To run the method, click the button. Start Method Scan The method will end when it reaches a step in which the speed setting is 0 rpm and the delay condition is 0:00. Using Previously Saved Scan Settings To open and run a previously saved scan settings file, follow the steps below.

  • Page 83: Ending A Run

    System Operation Ending a Run Ending a Run Select , or press on the instrument control panel, to end a run in the HOLD mode. File > Stop XL STOP Timed runs will end automatically when the set time has elapsed. After the run has stopped, press on the instrument control panel to vent the chamber.
  • Page 84 System Operation Ending a Run 4-30 PN LXLAI-IM-10AB...

  • Page 85: Troubleshooting And Maintenance

    Troubleshooting and maintenance not covered in this manual should be handled by Beckman Coulter Field Service. Refer to the analytical rotor manual (LXL/A-TB-003) and Chemical Resistances (publication IN-175) for instructions on the care of rotors and their accessories.
  • Page 86 Troubleshooting and Maintenance Retrieving Your Sample In Case of Power Failure WARNING NEVER attempt to slow or stop the rotor by hand. WARNING The following procedure should be performed only when absolutely necessary and only by qualified service personnel. If equipped with a printer, tear off any paper extending from the printer before trying to slide the control head cover off the instrument.
  • Page 87 Troubleshooting and Maintenance Retrieving Your Sample In Case of Power Failure To open the top cover, insert a #2 (0.25-inch diameter) Phillips-head screwdriver into the hole located in the front, center of the top cover (see Figure 5.2). Turn the screwdriver counterclockwise (to the left) until the screw bottoms out. Then, to release the latch, push the screwdriver inward.
  • Page 88 Troubleshooting and Maintenance Retrieving Your Sample In Case of Power Failure Figure 5.3 Interior View of Instrument (Panel Removed) Spring-loaded Interlock Pin Door Lock System Vacuum Solenoid Screw Fan housing About 10 seconds after the hissing noise stops, turn the setscrew to the left (counterclockwise) until it stops.

  • Page 89: Error And Diagnostic Messages

    If the message persists, or there are no suggested actions, call Beckman Coulter Field Service for assistance. Table 5.1 Diagnostic Messages Appearing on the PC Screen Error Message...
  • Page 90 Scan Options dialog box, and then performing one scan. (See Section 4 for scan setup instructions.) If problem persists, call Beckman Coulter Field Service. Radial calibration failure Radial calibration failed Ensure that the correct counterbalance is in...
  • Page 91 The instrument received a corrupt Check the cable connecting the PC and the message from the PC instrument for loose connections or damage. Call Beckman Coulter Field Service if either of Invalid communications The PC created a corrupt message these messages recurs.

  • Page 92: Control Panel Diagnostics

    Beckman Coulter Field Service for assistance. Table 5.2 lists the diagnostic category, possible cause of the condition, and appropriate user action. If there is no user action or you cannot correct the problem, call Beckman Coulter Field Service. PN LXLAI-IM-10AB...

  • Page 93: In Case Microsoft Windows Locks Up

    DRIVE Tachometer failure Call Beckman Coulter Field Service. Do not attempt to start another run until the instrument is serviced. VACUUM Vacuum system failure; or moisture Wipe the chamber with a cloth to remove moisture;...

  • Page 94: Maintenance

    Do not attempt to clean the UV light detector slit If spillage has affected the detector, call Beckman Coulter Field Service. Store the monochromator in the centrifuge chamber with the door closed, or in the wooden storage box provided.

  • Page 95: Cleaning The Monochromator Window

    NOTE Kit (368488) is for use only with XL-A and XL-I instruments manufactured after March 1995. The cleaning procedure below can be performed only after a Beckman Coulter field service representative has installed lamp assembly (368449). 5-11...

  • Page 96: Installing The Monochromator

    Troubleshooting and Maintenance Cleaning Figure 5.5 Monochromator Window Assembly and Window Installation and Removal Tool Window Tool (367809) Window Assembly (367810) Cleaning Procedure The window assembly sits just beneath the monochromator in the instrument chamber. To clean the window, Open the chamber door. Carefully remove the monochromator by unscrewing the red holding ring and lifting the monochromator out of the chamber.

  • Page 97: Decontamination

    Ethanol (70%) may be used on both these surfaces. See Chemical Resistances for more information regarding chemical resistance of instrument and accessory material. While Beckman Coulter has tested these methods and found that they do not damage the instrument, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

  • Page 98: Running Disk Cleanup

    Troubleshooting and Maintenance Hard Drive Maintenance Running Disk Cleanup Select Start > Programs > Accessories > System Tools > DiskCleanup Specify that you want the Disk Cleanup to check your hard drive and click the button. Start As the scan progresses, Disk Cleanup will display files for deletion and prompt you to delete the files or choose not to.

  • Page 99: Variations Between Results Obtained With Absorbance Optics And Bench-Top Spectrophotometers

    Troubleshooting and Maintenance Variations Between Results Obtained with Absorbance Optics and Bench-top Spectrophotometers Specify that you want to defragment your hard drive and click Disk Defragmenter will check the percentage of drive fragmentation and tell you whether or not the drive needs to be defragmented. You can then either start defragmentation by clicking the button, or click to exit the...

  • Page 100: Wavelength Scans Of A Windowless Cell In A Calibrated Xl-A Or

    Troubleshooting and Maintenance Wavelength Accuracy Check Figure 5.7 Wavelength Scans of a Windowless Cell in a Calibrated XL-A or XL-I 22.6 3000 0002597 1.864E07 6.56 1 22.0 3000 0179616 3.568E16 6.56 1 Lamp Intensity Check Lamp Intensity Check A00001.WA3 A00003.WA3 18059.57 4236.09 137.89...
  • Page 101 Troubleshooting and Maintenance Wavelength Accuracy Check Click the button. Detail Change the default wavelength step size to 1 and the data folder name to Wavecal. Click Click the button. Click the box. Options Acquire intensity data instead of OD data Click the button.

  • Page 102: Printer Maintenance (If Equipped)

    Troubleshooting and Maintenance Printer Maintenance (If Equipped) Compare the scan with the scan shown at the left in Figure 5.1. There should be a peak at 230 nm and another at 527 nm. If the intensity of the 230-nm peak is below 6000, the lamp probably needs cleaning. earlier in this section.

  • Page 103: Changing The Printer Ribbon

    Troubleshooting and Maintenance Printer Maintenance (If Equipped) WARNING Be careful NOT to touch the print head immediately after printing as it can get very hot. Lift the grooved rod from the slots to remove the old paper roll. Discard the old paper roll but keep the rod. Trim the edge of the new paper at an angle, with the pointed edge on the right.
  • Page 104 Troubleshooting and Maintenance Printer Maintenance (If Equipped) WARNING Be careful NOT to touch the print head immediately after printing as it can get very hot. Remove the paper guide, clear plastic cutter, and two screws as a unit as shown in Figure 5.9.

  • Page 105: Inserting The New

    Troubleshooting and Maintenance Printer Maintenance (If Equipped) Figure 5.10 Inserting the New Ribbon Lever Ribbon Guide Driving Pins Empty Spool Holding the ribbon taut, slide the ribbon between the print head and the platen, wrap it around the black ribbon guide at the right end of the platen, then drop the spool onto the spool shaft. (You will need to release the lever to engage the spool.) You should hear a click as the spool falls into position.

  • Page 106: Storage And Transport

    Returning an Instrument Before returning an instrument or accessory for any reason, prior permission (a form) must be obtained from Beckman Coulter. Contact your local Beckman Coulter office to obtain the form and instructions for packaging and shipping. To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from pathogens and/or radioactivity.

  • Page 107: Supply List

    Supply List Supply List Call Beckman Coulter Sales (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter office or visit us on the web at www.beckmancoulter.com) for information on ordering parts and supplies. For your convenience, a partial list is given below.
  • Page 108 Troubleshooting and Maintenance Supply List Description Part Number Window Cleaning Kit 368488 contains window assembly (367810), window tool (367709), and lamp assembly (368449) Hand cleaner 927741 Silicone vacuum grease (1 oz) 335148 Solution 555 (1 qt) 339555 a. Used on instruments equipped with an onboard printer. 5-24 PN LXLAI-IM-10AB...

  • Page 109: Appendix A: Installation Requirements

    Requirements for Installation CAUTION Do not attempt to install or turn on power to the ProteomeLab XL-A or XL-I. Its purchase price includes installation by Beckman Coulter personnel. Installation by anyone other than an authorized Beckman Coulter representative invalidates any warranty covering the instrument.

  • Page 110: Space Requirements

    Mains (power) plug from the outlet receptacle. Space Requirements The space requirements for the ProteomeLab XL-A or XL-I instrument and data system are shown Figure A.2. A 5.1-cm (2-in.) clearance is required on both sides of the instrument, as the feet extend 5.1 cm (2 in.) beyond the instrument.

  • Page 111: Bio-Safety Level 3 Installation

    Installing the ProteomeLab XL-A/XL-I User Interface Software A Beckman Coulter Field Service representative will install the hardware and software required for system operation. The following procedures are provided in case you need to reload the user interface software for any reason.
  • Page 112 Installation Requirements Installing the ProteomeLab XL-A/XL-I User Interface Software Insert the XL-A/XL-I Windows Setup CD. by double-clicking the file from within Windows Explorer, or by Setup.exe Setup.exe choosing from the Add/Remove Programs window on the Control Panel. Setup.exe Follow the instructions in the Install Wizard.

  • Page 113: Adjusting The Screen Resolution

    Installation Requirements Installing the ProteomeLab XL-A/XL-I User Interface Software c. Select the button, and the installation will start copying the program files. Next d. Select to complete the setup and reboot the computer. Finish Adjusting the Screen Resolution The XL-A/XL-I user interface software was designed to run under Windows XP, with the screen resolution set to 1024 ...

  • Page 114: Uninstalling The Xl-A/Xl-I User Interface Software

    Installation Requirements Installing the ProteomeLab XL-A/XL-I User Interface Software Select the Display icon from the Control Panel. Under the Settings tab, set the Screen area to 1024 by 768 pixels. Click the button and set the Display font size to large fonts.

  • Page 115: Appendix B: Interference Theory

    APPENDIX B Interference Theory Description of Interference Theory Interference is a characteristic of all wave motion, whether they are water, sound, or light waves. It is easily seen in a pool of water, when two objects are dropped into the water near each other. Small waves or ripples spread out in a circle around the spot where each object was dropped.

  • Page 116: Thomas Young's Original Drawing Of A Two-Source Interference

    Interference Theory Description of Interference Theory Figure B.2 Thomas Young’s Original Drawing of a Two-Source Interference Pattern NOTE Letters C, D, E, and F mark regions of destructive interference in Figure B.2. Figure B.3 Interference Pattern Created by Two Slits Monochromatic Light Double Slits...

  • Page 117: Light From O Passes Through Slits M And N And Produces

    Interference Theory Description of Interference Theory Figure B.4 Light from O passes Through Slits M and N and Produces an Interference Pattern on Screen S Light Dark Light Dark Light Figure B.5 Vertical Fringe Displacement in the ProteomeLab XL-I Fringe Display Fringe displacement is a result of the different path lengths, from light source to detector, encountered by the two beams of light.

  • Page 118: References

    Interference Theory References References Conceptual Physics, Seventh Edition. Paul G. Hewitt, Harper Collins College Publishers, 1993. Choosing Which Optical System of the XL-A Analytical Ultracentrifuge to Use (publication A-1821). Thomas M. Laue, 1995. PN LXLAI-IM-10AB...

  • Page 119: Glossary

    (such as the files into convenient groups on a disk. A directory ProteomeLab XL-A or XL-I instrument). The XL-A/ is like a file drawer—it can hold a group of files, XL-I user interface software enables selection of or it can contain both files and subdirectories Com ports 1, 2, 3, and 4.
  • Page 120 Photomultiplier — An electron multiplier in which the first stage consists of photoelectric emission from a cathode. In the ProteomeLab XL-A or XL-I, the photomultiplier amplifies the light signal that it receives from the detector. RAM (Random Access Memory — The computer’s memory, in which both a program...
  • Page 121 Index on ultracentrifuge control panel, 5-8, absorbance data acquiring after a scan, absorbance optical system, 2-5, ending a run, 4-29 scan types, 2-5, equilibrium scans specifications, description, 2-5, 4-23 variation with spectrophotometer setup, absorbance, 4-23 results, 5-15 setup, interference, 4-11 wavelength accuracy check, 5-15 error messages, 5-5,...
  • Page 122 Index interference theory, changing paper, 5-18, 5-19 fringe pattern, changing ribbon, 5-19, 5-20 refractive index, radial calibration laser, absorbance system, 4-25 control settings, 3-8, 4-12, 4-13 interference system, 3-9, 4-6, 4-10 setup, 4-12 radial positions, set (interference system), 4-17 lens radial scans camera, equilibrium,...
  • Page 123 Index disks, 5-13, menu, installation, monitor, Windows, settings, space requirements, XL-I start method scan, 3-5, 4-22, 4-23 accel/decel profiles, 1-2, start single scan, 3-5, 4-7, 4-19, 4-21 control panel, 2-12, 2-13 start XL, 4-15 maintenance, 5-10 sterilization, 5-13 retrieving sample from, stop scan, 3-5, 4-28 space requirements,...
  • Page 124 Index Index-4...

  • Page 125: Beckman Coulter, Inc

    Any product claimed to be defective must, if requested by Beckman Coulter be returned to the factory, transportation charges prepaid, and will be returned to Buyer with the transportation charges collect unless the product is found to be defective, in which case Beckman Coulter will pay all transportation charges.
  • Page 126 2. The drive unit has not been subjected to unequal loading, improper rotor installation, corrosion from material spilled onto the hub or accumulated in the chamber of the instrument. 3. The drive unit has not been disassembled, modified, or repaired, except by Beckman Coulter personnel.
  • Page 127 English / Deutsch / Español / Français / Italiano / Portugués / Русский / 中文 / 日本語 / 한국어 Symbol Simbole Symbol символ Simbolo 符号 Title / Titel / Titulo / Titre / Titolo / Titulo / Название / 标题 / タイトル / 제목 Symbole 記号...
  • Page 128 • The Use of Cesium electronic pdf by request. electronic pdf by request. Chloride Curves • Gradient Materials • References • Glossary Available in electronic pdf or CD-ROM by request. www.beckmancoulter.com © 2014 Beckman Coulter, Inc. All Rights Reserved...

This manual is also suitable for:

Proteomelab xl-l

Table of Contents