Using Diascopic Illumination - Nikon SMZ25 Instruction Manual

12

Using Diascopic Illumination

The microscope system enables diascopic illumination when
used with the devices listed below. All of the devices use LEDs as
the light source and provide uniform illumination.
The image to be observed results from diascopic illumination
which passes through the sample and enters the objective.
Diascopic illumination is used for observing transparent
substances such as macromolecular compounds, fibers, and
glass and tissue sections and biological specimens in petri
dishes.
In addition, optional devices can be attached to enable dark-field
diascopic illumination and diascopic simplified/polarized
illumination. This allows for the observation of samples that are
difficult to be observed under ordinary bright-field diascopic
illumination such as transparent materials and crystals and
tissues and minute organisms in culture solutions.
See Chapter 4 "8 Diascopic Illuminator" and select the
appropriate device based on the objective of microscopy.
 P2-DBL LED Diascopic Illumination Base
 P2-DBF Fiber Diascopic Illumination Base
 P-DF LED Dark Field Unit
 P2-POL Simple Polarizing Attachment
Empty cube
When an epi-fluorescence attachment is mounted, check that the empty cube of the attachment is in the optical path.
OCC illumination
The P2-DBL LED Diascopic Illumination Base and P2-DBF Fiber Diascopic Illumination Base support OCC illumination
(oblique illumination). OCC illumination, which offers high contrast, is appropriate for microscopy of phase objects.
Chapter 3
Specific Operations
31
Diascopic illumination with the P2-DBL LED
Diascopic Illumination Base