P2-Dbf Fiber Diascopic Illumination Base - Nikon SMZ25 Instruction Manual

P2-DBF Fiber Diascopic Illumination Base

8.2
This illumination base contains an optical system for episcopic
illumination. Since the base supports OCC illumination,
high-contrast observation of transparent samples is enabled. The
C-FLED2 LED Light Source for Fiber Illuminator is used as the
light source. The fiber should be connected to the rear side of the
base. For details about using the light source, refer to the
instruction manual for the light source.
The stage plate of the base is large (180 mm dia.) and made of
transparent glass to enable more efficient work and easier
observation of samples in containers such as petri dishes. The
base has three indents for holding the base: front of the base's
top board and on the right and left rear.
The stage plate can be replaced with a stage having an X/Y shift
knob or a sliding stage. It is also possible to attach other devices
such as a fiber illuminator or diascopic polarizing device to
expand the base's functions.
 OCC (oblique coherent contrast) illumination
The fiber diascopic illumination base supports OCC illumination. OCC illumination is an oblique illumination method for
observing the phase objects, where a sample is illuminated with a coherent illuminating light obliquely by shielding a part
of the optical path using a shading plate. By this illumination method, a colorless, transparent sample can be observed
with relief and contrast.
To adjust the OCC contrast, turn the OCC adjustment knob. Adjust until the optimum observation state is obtained in
combination with the selected magnification and phase amount of the sample. In addition, since the OCC adjustment
knob has a scale, the position of the knob can be reproduced.
 Condenser lens
The base contains condenser lenses for low/high-magnification microscopy. Switch the condenser lens based on the
objective being used to ensure uniform diascopic illumination. A wide range of observation magnifications are supported.
To switch the condenser lens, slide the condenser lens switching lever to the right or left.
Slide the lens switching lever to the right: For low magnification; objective lens 0.5x to 1x
Slide the lens switching lever to the left:
When the condenser lens is switched, the built-in prism sheet is automatically switched and the shading adjusted.
P2-SHR Plan Apo 0.5X plus condenser lens for low magnification
P2-SHR Plan Apo 01X plus condenser lens for low magnification
P2-SHR Plan Apo 1.6X plus condenser lens for high magnification
P2-SHR Plan Apo 2X plus condenser lens for high magnification
* Shading may occur in the low zoom magnification range.
 Filter
The base has three different filters: NCB11, ND4, and ND16. The individual filters can be inserted into and removed from
the optical path separately. To insert a filter into the optical path, push in the filter IN/OUT lever.
45-mm dia. filter
A 45-mm dia. filter (no thicker than 2 mm) can be dropped onto the filter dropping ring (attached to the base optical path
immediately under the grass plate).
Chapter 4
Functions and Operations of the Devices
For high magnification; objective lens 1.6x to 2x
Compatible objectives and recommended zoom magnification
Objective
P2-DBF Fiber Diascopic Illumination Base
Zoom magnification
Bright-field diascopic
illumination
1.5x -
Entire area
Entire area
2.0x -
56
Fiber
Fiber
fixing screw
Filter IN/OUT lever
OCC adjustment knob
Condenser lens switching lever
OCC illumination
4.5x -
4.0x -
4.0x -
6.0x-