P2-Eflm Motorized Epi Fluorescence Attachment - Nikon SMZ25 Instruction Manual

Bright-field episcopic observation
This is useful for observing the entire image of the opaque specimen during fluorescence observation. Although the
P2-EFLBF Filter Cube contains the UV blocking filter, adjust the brightness of the illuminator so that illumination is not too
bright. When observing light reflected from the specimen with a mirror-like surface, the brightness on the right path differs
from that on the left optical path. Also, duplicated images may be produced by internal half mirror.
OCC light shielding plate
When using an epi-fluorescence attachment with a diascopic illumination base, Nikon recommends closing the OCC light
shielding plate on the dia-illumination completely. This can decrease background noise caused by intrinsic fluorescence in
the diascopic illuminator.
For details about using the light source, refer to the instruction manual for the light source.

P2-EFLM Motorized Epi Fluorescence Attachment

9.2
The motorized epi-fluorescence illuminator is attached between
the zooming body and tube. A HG precentered fiber illuminator is
used as the light source. The light coming from the light source is
led into the device by the HG fiber and then passed through the
filter cube attached to the internal turret for epi-fl illumination.
The turret can be attached with up to four filter cubes; the
addresses of the attachment positions are FL1 to FL4. For the
attachable filter cube types, those for fluorescence, bright-field,
and empty are available. On the remote control, press the filter
cube switching buttons (FL1 to FL4) to specify the turret address
of the filter cube to be placed into the optical path. The LED of the
pressed button lights so that the turret address on the optical path
can be identified. The attachment has a turret address check
window on the right front so that the turret address on the optical
path can be identified.
For systems where a control box has been connected, the
information about the turret address on the optical path is sent to
the control box via a motorized focus unit or relay box, and then
transmitted to the DS-L3 DS Camera Control Unit and personal
computer.
CAUTION
 Do not use the epi-fluorescence attachment while the filter cube replacement cover is open. Harmful light including ultraviolet
light may leak and affect fluorescence observation.
 Immediately after diascopic observation using an epi-fluorescence attachment, the front face of the empty cube may become
hot, resulting in burns. Do not touch the front face of the empty cube. Replace the empty cube several minutes after finishing
the diascopic observation.
Be sure to attach four cubes to the turret.
Attach an empty cube to the position to which no filter cube is attached.
Chapter 4
Functions and Operations of the Devices
P2-EFLM Motorized Epi Fluorescence Attachment
59
Filter cube indication
label
(label attachment
position)
Filter cube
replacement hole
cover
Turret address check
window